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Using nile red-adsorbed gold nanoparticles to locate glutathione within erythrocytes.
Langmuir. 2005 Nov 08; 21(23):10676-83.L

Abstract

An aqueous solution of Nile Red (NR)-absorbed 32-nm gold nanoparticles (AuNPs) have been used to sense glutathione (GSH). When the NR product is displaced by GSH on the AuNP surface, the fluorescence of the solution increases and the AuNPs aggregate. To determine the concentration and distribution of GSH within erythrocyte cells, a homemade fluorescence and scattering microscope was constructed. This system allows monitoring, within individual cells, of the uptake and transportation of the NRAuNPs and the displacement of the NR product from the NRAuNP surface by GSH. The fluorescence and scattering images clearly indicate the location of GSH inside the cells; these findings are supported by images recorded using 2,3-naphthalenedicarboxaldehyde, which is a highly selective fluorogenic reagent for GSH. Microscopic fluorescence measurements of the NRAuNPs revealed that the GSH concentration inside erythrocyte cells is 1.30 +/- 0.31 mM. To confirm this result, lysed erythrocyte cells were analyzed by applying capillary electrophoresis in conjunction with laser-induced fluorescence using NRAuNPs; accordingly, the average GSH concentration in a single erythrocyte cell was determined to be 1.32 +/- 0.06 mM.

Authors+Show Affiliations

Tseng WL
Department of Chemistry, National Taiwan University, Taipei.
Lee KH
No affiliation info available
Chang HT
No affiliation info available

MeSH

Electrophoresis, CapillaryErythrocytesGlutathioneGoldMetal NanoparticlesMicroscopy, FluorescenceOxazines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16262336

Citation

Tseng, Wei-Lung, et al. "Using Nile Red-adsorbed Gold Nanoparticles to Locate Glutathione Within Erythrocytes." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 21, no. 23, 2005, pp. 10676-83.
Tseng WL, Lee KH, Chang HT. Using nile red-adsorbed gold nanoparticles to locate glutathione within erythrocytes. Langmuir. 2005;21(23):10676-83.
Tseng, W. L., Lee, K. H., & Chang, H. T. (2005). Using nile red-adsorbed gold nanoparticles to locate glutathione within erythrocytes. Langmuir : the ACS Journal of Surfaces and Colloids, 21(23), 10676-83.
Tseng WL, Lee KH, Chang HT. Using Nile Red-adsorbed Gold Nanoparticles to Locate Glutathione Within Erythrocytes. Langmuir. 2005 Nov 8;21(23):10676-83. PubMed PMID: 16262336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Using nile red-adsorbed gold nanoparticles to locate glutathione within erythrocytes. AU - Tseng,Wei-Lung, AU - Lee,Kun-Hong, AU - Chang,Huan-Tsung, PY - 2005/11/3/pubmed PY - 2007/5/5/medline PY - 2005/11/3/entrez SP - 10676 EP - 83 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 21 IS - 23 N2 - An aqueous solution of Nile Red (NR)-absorbed 32-nm gold nanoparticles (AuNPs) have been used to sense glutathione (GSH). When the NR product is displaced by GSH on the AuNP surface, the fluorescence of the solution increases and the AuNPs aggregate. To determine the concentration and distribution of GSH within erythrocyte cells, a homemade fluorescence and scattering microscope was constructed. This system allows monitoring, within individual cells, of the uptake and transportation of the NRAuNPs and the displacement of the NR product from the NRAuNP surface by GSH. The fluorescence and scattering images clearly indicate the location of GSH inside the cells; these findings are supported by images recorded using 2,3-naphthalenedicarboxaldehyde, which is a highly selective fluorogenic reagent for GSH. Microscopic fluorescence measurements of the NRAuNPs revealed that the GSH concentration inside erythrocyte cells is 1.30 +/- 0.31 mM. To confirm this result, lysed erythrocyte cells were analyzed by applying capillary electrophoresis in conjunction with laser-induced fluorescence using NRAuNPs; accordingly, the average GSH concentration in a single erythrocyte cell was determined to be 1.32 +/- 0.06 mM. SN - 0743-7463 UR - https://www.unboundmedicine.com/medline/citation/16262336/Using_nile_red_adsorbed_gold_nanoparticles_to_locate_glutathione_within_erythrocytes_ DB - PRIME DP - Unbound Medicine ER -