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Accumulation of polyphosphates and expression of high molecular weight exopolyphosphatase in the yeast Saccharomyces cerevisiae.
Biochemistry (Mosc). 2005 Sep; 70(9):980-5.B

Abstract

The effect of cultivation time and concentration of inorganic phosphate (P(i)) in the culture medium on the accumulation of polyphosphates (polyP) and the activity of two cytosolic exopolyphosphatases of the yeast Saccharomyces cerevisiae was studied: an exopolyphosphatase of 40 kD encoded by PPX1 and a high molecular weight exopolyphosphatase encoded by another gene. Depletion of polyP in the cells on P(i) starvation is a signal factor for the accumulation of polyP after the subsequent addition of 5-20 mM P(i) and glucose to the cells or spheroplasts. A high activity of both exopolyphosphatases does not prevent the accumulation of polyP. The expression of the high molecular weight exopolyphosphatase is due to the acceleration of metabolism in cells that have reached the stage of growth deceleration on the addition of P(i) and glucose or complete culture medium. This process may occur independently from the accumulation of polyP. The activity of exopolyphosphatase PPX1 depends less on the mentioned factors, decreasing 10-fold only under conditions of phosphate surplus at the stationary growth stage.

Authors+Show Affiliations

Kulakovskaya TV
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia. alla@ibpm.pushchino.ru
Andreeva NA
No affiliation info available
Trilisenko LV
No affiliation info available
Suetin SV
No affiliation info available
Vagabov VM
No affiliation info available
Kulaev IS
No affiliation info available

MeSH

Acid Anhydride HydrolasesCulture MediaCytosolMolecular WeightPermeabilityPhosphatesPolyphosphatesSaccharomyces cerevisiaeSpheroplastsTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16266267

Citation

Kulakovskaya, T V., et al. "Accumulation of Polyphosphates and Expression of High Molecular Weight Exopolyphosphatase in the Yeast Saccharomyces Cerevisiae." Biochemistry. Biokhimiia, vol. 70, no. 9, 2005, pp. 980-5.
Kulakovskaya TV, Andreeva NA, Trilisenko LV, et al. Accumulation of polyphosphates and expression of high molecular weight exopolyphosphatase in the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2005;70(9):980-5.
Kulakovskaya, T. V., Andreeva, N. A., Trilisenko, L. V., Suetin, S. V., Vagabov, V. M., & Kulaev, I. S. (2005). Accumulation of polyphosphates and expression of high molecular weight exopolyphosphatase in the yeast Saccharomyces cerevisiae. Biochemistry. Biokhimiia, 70(9), 980-5.
Kulakovskaya TV, et al. Accumulation of Polyphosphates and Expression of High Molecular Weight Exopolyphosphatase in the Yeast Saccharomyces Cerevisiae. Biochemistry (Mosc). 2005;70(9):980-5. PubMed PMID: 16266267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accumulation of polyphosphates and expression of high molecular weight exopolyphosphatase in the yeast Saccharomyces cerevisiae. AU - Kulakovskaya,T V, AU - Andreeva,N A, AU - Trilisenko,L V, AU - Suetin,S V, AU - Vagabov,V M, AU - Kulaev,I S, PY - 2005/11/4/pubmed PY - 2006/1/31/medline PY - 2005/11/4/entrez SP - 980 EP - 5 JF - Biochemistry. Biokhimiia JO - Biochemistry (Mosc) VL - 70 IS - 9 N2 - The effect of cultivation time and concentration of inorganic phosphate (P(i)) in the culture medium on the accumulation of polyphosphates (polyP) and the activity of two cytosolic exopolyphosphatases of the yeast Saccharomyces cerevisiae was studied: an exopolyphosphatase of 40 kD encoded by PPX1 and a high molecular weight exopolyphosphatase encoded by another gene. Depletion of polyP in the cells on P(i) starvation is a signal factor for the accumulation of polyP after the subsequent addition of 5-20 mM P(i) and glucose to the cells or spheroplasts. A high activity of both exopolyphosphatases does not prevent the accumulation of polyP. The expression of the high molecular weight exopolyphosphatase is due to the acceleration of metabolism in cells that have reached the stage of growth deceleration on the addition of P(i) and glucose or complete culture medium. This process may occur independently from the accumulation of polyP. The activity of exopolyphosphatase PPX1 depends less on the mentioned factors, decreasing 10-fold only under conditions of phosphate surplus at the stationary growth stage. SN - 0006-2979 UR - https://www.unboundmedicine.com/medline/citation/16266267/Accumulation_of_polyphosphates_and_expression_of_high_molecular_weight_exopolyphosphatase_in_the_yeast_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -