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Preservation of pressure-induced cutaneous vasodilation by limiting oxidative stress in short-term diabetic mice.
Cardiovasc Res. 2006 Jan; 69(1):245-52.CR

Abstract

OBJECTIVE

Pressure-induced vasodilation (PIV) allows skin blood flow to increase in response to locally applied pressure and may be protective against pressure ulcers. We previously showed that PIV was absent in 1-week diabetic mice exhibiting no neuropathy. Our aim was to determine whether the diabetes-induced PIV alteration could be prevented.

METHODS AND RESULTS

Diabetic mice received no treatment or a daily treatment with either sorbinil, alagebrium or alpha-lipoic acid (LPA) for 1 week. Laser Doppler flowmetry was used to evaluate PIV as well as endothelium-dependent vasodilation following iontophoretic delivery of acetylcholine (ACh). The effect of each treatment on oxidative stress was examined by plasma 8-isoprostane assay. LPA was the sole treatment to prevent both PIV and ACh vasodilation alterations, with a significant reduction of oxidative stress in diabetic mice. Both PIV and ACh-vasodilation were abolished in LPA-treated diabetic mice following injection of Nomega-nitro-L-arginine (p<0.05). In contrast, alagebrium and sorbinil prevented neither diabetes-induced PIV abolition nor endothelial alteration.

CONCLUSIONS

LPA treatment significantly reduced the oxidative stress and was able to preserve endothelial nitric oxide availability in the cutaneous microcirculation and then to preserve the PIV response in diabetic mice. LPA treatment could play a key role in limiting the risk of pressure-induced cutaneous ulcer during diabetes.

Authors+Show Affiliations

Laboratory of physiology, UMR CNRS 6188, Medical school, 49045 Angers cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16266692

Citation

Demiot, Claire, et al. "Preservation of Pressure-induced Cutaneous Vasodilation By Limiting Oxidative Stress in Short-term Diabetic Mice." Cardiovascular Research, vol. 69, no. 1, 2006, pp. 245-52.
Demiot C, Fromy B, Saumet JL, et al. Preservation of pressure-induced cutaneous vasodilation by limiting oxidative stress in short-term diabetic mice. Cardiovasc Res. 2006;69(1):245-52.
Demiot, C., Fromy, B., Saumet, J. L., & Sigaudo-Roussel, D. (2006). Preservation of pressure-induced cutaneous vasodilation by limiting oxidative stress in short-term diabetic mice. Cardiovascular Research, 69(1), 245-52.
Demiot C, et al. Preservation of Pressure-induced Cutaneous Vasodilation By Limiting Oxidative Stress in Short-term Diabetic Mice. Cardiovasc Res. 2006;69(1):245-52. PubMed PMID: 16266692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preservation of pressure-induced cutaneous vasodilation by limiting oxidative stress in short-term diabetic mice. AU - Demiot,Claire, AU - Fromy,Bérengère, AU - Saumet,Jean Louis, AU - Sigaudo-Roussel,Dominique, Y1 - 2005/11/02/ PY - 2005/07/11/received PY - 2005/08/29/revised PY - 2005/09/12/accepted PY - 2005/11/4/pubmed PY - 2006/5/4/medline PY - 2005/11/4/entrez SP - 245 EP - 52 JF - Cardiovascular research JO - Cardiovasc. Res. VL - 69 IS - 1 N2 - OBJECTIVE: Pressure-induced vasodilation (PIV) allows skin blood flow to increase in response to locally applied pressure and may be protective against pressure ulcers. We previously showed that PIV was absent in 1-week diabetic mice exhibiting no neuropathy. Our aim was to determine whether the diabetes-induced PIV alteration could be prevented. METHODS AND RESULTS: Diabetic mice received no treatment or a daily treatment with either sorbinil, alagebrium or alpha-lipoic acid (LPA) for 1 week. Laser Doppler flowmetry was used to evaluate PIV as well as endothelium-dependent vasodilation following iontophoretic delivery of acetylcholine (ACh). The effect of each treatment on oxidative stress was examined by plasma 8-isoprostane assay. LPA was the sole treatment to prevent both PIV and ACh vasodilation alterations, with a significant reduction of oxidative stress in diabetic mice. Both PIV and ACh-vasodilation were abolished in LPA-treated diabetic mice following injection of Nomega-nitro-L-arginine (p<0.05). In contrast, alagebrium and sorbinil prevented neither diabetes-induced PIV abolition nor endothelial alteration. CONCLUSIONS: LPA treatment significantly reduced the oxidative stress and was able to preserve endothelial nitric oxide availability in the cutaneous microcirculation and then to preserve the PIV response in diabetic mice. LPA treatment could play a key role in limiting the risk of pressure-induced cutaneous ulcer during diabetes. SN - 0008-6363 UR - https://www.unboundmedicine.com/medline/citation/16266692/Preservation_of_pressure_induced_cutaneous_vasodilation_by_limiting_oxidative_stress_in_short_term_diabetic_mice_ L2 - https://academic.oup.com/cardiovascres/article-lookup/doi/10.1016/j.cardiores.2005.09.005 DB - PRIME DP - Unbound Medicine ER -