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Protective effects of N-acetylcysteine on the peroxidative changes of rat lungs exposed to inhalation of thinners.
Respirology. 2005 Nov; 10(5):615-9.R

Abstract

OBJECTIVE

Long-term inhalation of thinners may cause damage, both to the lungs and to other organ systems. It causes cellular damage via formation of reactive oxygen species. The lung is protected from oxidative stress by the glutathione (GSH) antioxidant system which can be augmented by the thiol drug, N-acetylcysteine (NAC). This study investigated the protective effect of NAC on peroxidative changes in rat lungs exposed to inhalation of thinners for 8 weeks.

METHODOLOGY

Seventy-two male Wistar albino rats were used and divided into two groups: one group inhaled only thinners (TI), while the other inhaled TI plus NAC. Rats in the TI and TI + NAC groups were divided into four subgroups (each consisting of eight rats) according to the duration of exposure to TI: 2, 4, 6 and 8 weeks. A control group (n = 7) of rats inhaled neither TI nor NAC. Malondialdehyde (MDA) and GSH levels, and superoxide dismutase (SOD) activities were determined in the lung tissues. Histopathological findings were evaluated as acute and chronic changes in the alveoli and interstitium in the TI and TI + NAC groups and compared with those in the control group.

RESULTS

While tissue MDA levels in the groups inhaling TI for 4, 6 and 8 weeks were significantly higher than those in the control groups (P < 0.01, P < 0.01, P < 0.0001, respectively), GSH levels were significantly lower (P < 0.05, P < 0.01, P < 0.01, respectively). Tissue SOD activities in the groups inhaling TI for 6 and 8 weeks were significantly lower than those in the control group (P < 0.05, P < 0.01, respectively). In the TI group, MDA levels were significantly increased (P < 0.01) with increasing duration of inhalation (from the second week through to the eighth week), while GSH levels and SOD activities were significantly decreased (P < 0.01, P < 0.01). Tissue MDA levels were significantly lower in the TI + NAC groups across all inhalation periods, when compared with the TI groups (P < 0.01, P < 0.0001, P < 0.0001, P < 0.0001, respectively). Tissue GSH levels in the TI + NAC groups were significantly higher than those of the TI groups (respective values: P < 0.05, P < 0.01, P < 0.01, P < 0.0001). Tissue SOD activities in the TI + NAC groups were significantly higher than those of the TI groups (respective values: P < 0.05, P < 0.0001, P < 0.05, P < 0.0001). Pathological examinations with light microscopy did not show any beneficial effect of NAC application in terms of deferring or alleviating the negative effects of TI.

CONCLUSIONS

Thinners are agents that cause imbalance between oxidants and antioxidants produced by aerobic cellular systems. This imbalance between oxidant and antioxidant systems is decreased by the effect of NAC. However, ultrastructural studies may be needed to substantiate this evidence morphologically, as light microscopy was inconclusive.

Authors+Show Affiliations

Department of Clinical Biochemistry, Faculty of Medicine, Kocaeli University, Turkey. mozden@superonline.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

16268915

Citation

Dillioglugil, Meltem Ozlen, et al. "Protective Effects of N-acetylcysteine On the Peroxidative Changes of Rat Lungs Exposed to Inhalation of Thinners." Respirology (Carlton, Vic.), vol. 10, no. 5, 2005, pp. 615-9.
Dillioglugil MO, Ilgazli A, Maral H, et al. Protective effects of N-acetylcysteine on the peroxidative changes of rat lungs exposed to inhalation of thinners. Respirology. 2005;10(5):615-9.
Dillioglugil, M. O., Ilgazli, A., Maral, H., Sengul, C., Ozdemir, G., & Ercin, C. (2005). Protective effects of N-acetylcysteine on the peroxidative changes of rat lungs exposed to inhalation of thinners. Respirology (Carlton, Vic.), 10(5), 615-9.
Dillioglugil MO, et al. Protective Effects of N-acetylcysteine On the Peroxidative Changes of Rat Lungs Exposed to Inhalation of Thinners. Respirology. 2005;10(5):615-9. PubMed PMID: 16268915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of N-acetylcysteine on the peroxidative changes of rat lungs exposed to inhalation of thinners. AU - Dillioglugil,Meltem Ozlen, AU - Ilgazli,Ahmet, AU - Maral,Hale, AU - Sengul,Canan, AU - Ozdemir,Gulsen, AU - Ercin,Cengiz, PY - 2005/11/5/pubmed PY - 2006/2/8/medline PY - 2005/11/5/entrez SP - 615 EP - 9 JF - Respirology (Carlton, Vic.) JO - Respirology VL - 10 IS - 5 N2 - OBJECTIVE: Long-term inhalation of thinners may cause damage, both to the lungs and to other organ systems. It causes cellular damage via formation of reactive oxygen species. The lung is protected from oxidative stress by the glutathione (GSH) antioxidant system which can be augmented by the thiol drug, N-acetylcysteine (NAC). This study investigated the protective effect of NAC on peroxidative changes in rat lungs exposed to inhalation of thinners for 8 weeks. METHODOLOGY: Seventy-two male Wistar albino rats were used and divided into two groups: one group inhaled only thinners (TI), while the other inhaled TI plus NAC. Rats in the TI and TI + NAC groups were divided into four subgroups (each consisting of eight rats) according to the duration of exposure to TI: 2, 4, 6 and 8 weeks. A control group (n = 7) of rats inhaled neither TI nor NAC. Malondialdehyde (MDA) and GSH levels, and superoxide dismutase (SOD) activities were determined in the lung tissues. Histopathological findings were evaluated as acute and chronic changes in the alveoli and interstitium in the TI and TI + NAC groups and compared with those in the control group. RESULTS: While tissue MDA levels in the groups inhaling TI for 4, 6 and 8 weeks were significantly higher than those in the control groups (P < 0.01, P < 0.01, P < 0.0001, respectively), GSH levels were significantly lower (P < 0.05, P < 0.01, P < 0.01, respectively). Tissue SOD activities in the groups inhaling TI for 6 and 8 weeks were significantly lower than those in the control group (P < 0.05, P < 0.01, respectively). In the TI group, MDA levels were significantly increased (P < 0.01) with increasing duration of inhalation (from the second week through to the eighth week), while GSH levels and SOD activities were significantly decreased (P < 0.01, P < 0.01). Tissue MDA levels were significantly lower in the TI + NAC groups across all inhalation periods, when compared with the TI groups (P < 0.01, P < 0.0001, P < 0.0001, P < 0.0001, respectively). Tissue GSH levels in the TI + NAC groups were significantly higher than those of the TI groups (respective values: P < 0.05, P < 0.01, P < 0.01, P < 0.0001). Tissue SOD activities in the TI + NAC groups were significantly higher than those of the TI groups (respective values: P < 0.05, P < 0.0001, P < 0.05, P < 0.0001). Pathological examinations with light microscopy did not show any beneficial effect of NAC application in terms of deferring or alleviating the negative effects of TI. CONCLUSIONS: Thinners are agents that cause imbalance between oxidants and antioxidants produced by aerobic cellular systems. This imbalance between oxidant and antioxidant systems is decreased by the effect of NAC. However, ultrastructural studies may be needed to substantiate this evidence morphologically, as light microscopy was inconclusive. SN - 1323-7799 UR - https://www.unboundmedicine.com/medline/citation/16268915/Protective_effects_of_N_acetylcysteine_on_the_peroxidative_changes_of_rat_lungs_exposed_to_inhalation_of_thinners_ L2 - https://doi.org/10.1111/j.1440-1843.2005.00758.x DB - PRIME DP - Unbound Medicine ER -