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Vasodilatory and anti-inflammatory effects of the aqueous extract of rhubarb via a NO-cGMP pathway.
Life Sci. 2006 Feb 28; 78(14):1550-7.LS

Abstract

While conducting an in vitro screen of various medicinal plant extracts, an aqueous extract of rhubarb (Rheum undulatum L, AR) was found to exhibit a distinct vasorelaxant activity. AR induced a concentration-dependent relaxation of the phenylephrine-precontracted aorta. This effect disappeared with the removal of functional endothelium. Pretreatment of the aortic tissues with N(G)-nitro-L-arginine methyl ester (L-NAME), methylene blue, or 1H-[1,2,4]-oxadiazole-[4,3-alpha]-quinoxalin-1-one (ODQ) inhibited the relaxation induced by AR. Incubation of human umbilical vein endothelial cells (HUVECs) with AR increased the production of cGMP in a dose-dependent manner, but this effect was blocked by pretreatment with L-NAME and ODQ, respectively. AR treatment attenuated TNF-alpha-induced NF-kappaB p65 translocation in HUVECs in a dose-dependent manner. In addition, AR suppressed the expression levels of adhesion molecules including ICAM-1 and VCAM-1 induced by TNF-alpha in HUVECs. TNF-alpha-induced MCP-1 expression was also attenuated by the addition of AR. This attenuation was blocked by pretreatment with either L-NAME or ODQ. AR treatment inhibited cellular adhesion of U937 cells onto HUVECs induced by TNF-alpha. Taken together, the present study suggests that AR dilates vascular smooth muscle and suppresses the vascular inflammatory process via endothelium-dependent NO/cGMP signaling.

Authors+Show Affiliations

Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16269157

Citation

Moon, Mi Kyoung, et al. "Vasodilatory and Anti-inflammatory Effects of the Aqueous Extract of Rhubarb Via a NO-cGMP Pathway." Life Sciences, vol. 78, no. 14, 2006, pp. 1550-7.
Moon MK, Kang DG, Lee JK, et al. Vasodilatory and anti-inflammatory effects of the aqueous extract of rhubarb via a NO-cGMP pathway. Life Sci. 2006;78(14):1550-7.
Moon, M. K., Kang, D. G., Lee, J. K., Kim, J. S., & Lee, H. S. (2006). Vasodilatory and anti-inflammatory effects of the aqueous extract of rhubarb via a NO-cGMP pathway. Life Sciences, 78(14), 1550-7.
Moon MK, et al. Vasodilatory and Anti-inflammatory Effects of the Aqueous Extract of Rhubarb Via a NO-cGMP Pathway. Life Sci. 2006 Feb 28;78(14):1550-7. PubMed PMID: 16269157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vasodilatory and anti-inflammatory effects of the aqueous extract of rhubarb via a NO-cGMP pathway. AU - Moon,Mi Kyoung, AU - Kang,Dae Gill, AU - Lee,Jun Kyong, AU - Kim,Jin Sook, AU - Lee,Ho Sub, Y1 - 2005/11/02/ PY - 2005/03/04/received PY - 2005/07/15/accepted PY - 2005/11/5/pubmed PY - 2006/4/15/medline PY - 2005/11/5/entrez SP - 1550 EP - 7 JF - Life sciences JO - Life Sci VL - 78 IS - 14 N2 - While conducting an in vitro screen of various medicinal plant extracts, an aqueous extract of rhubarb (Rheum undulatum L, AR) was found to exhibit a distinct vasorelaxant activity. AR induced a concentration-dependent relaxation of the phenylephrine-precontracted aorta. This effect disappeared with the removal of functional endothelium. Pretreatment of the aortic tissues with N(G)-nitro-L-arginine methyl ester (L-NAME), methylene blue, or 1H-[1,2,4]-oxadiazole-[4,3-alpha]-quinoxalin-1-one (ODQ) inhibited the relaxation induced by AR. Incubation of human umbilical vein endothelial cells (HUVECs) with AR increased the production of cGMP in a dose-dependent manner, but this effect was blocked by pretreatment with L-NAME and ODQ, respectively. AR treatment attenuated TNF-alpha-induced NF-kappaB p65 translocation in HUVECs in a dose-dependent manner. In addition, AR suppressed the expression levels of adhesion molecules including ICAM-1 and VCAM-1 induced by TNF-alpha in HUVECs. TNF-alpha-induced MCP-1 expression was also attenuated by the addition of AR. This attenuation was blocked by pretreatment with either L-NAME or ODQ. AR treatment inhibited cellular adhesion of U937 cells onto HUVECs induced by TNF-alpha. Taken together, the present study suggests that AR dilates vascular smooth muscle and suppresses the vascular inflammatory process via endothelium-dependent NO/cGMP signaling. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/16269157/Vasodilatory_and_anti_inflammatory_effects_of_the_aqueous_extract_of_rhubarb_via_a_NO_cGMP_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(05)00962-8 DB - PRIME DP - Unbound Medicine ER -