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Fluorescent proteins as a toolkit for in vivo imaging.
Trends Biotechnol. 2005 Dec; 23(12):605-13.TB

Abstract

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria, and its mutant variants, are the only fully genetically encoded fluorescent probes available and they have proved to be excellent tools for labeling living specimens. Since 1999, numerous GFP homologues have been discovered in Anthozoa, Hydrozoa and Copepoda species, demonstrating the broad evolutionary and spectral diversity of this protein family. Mutagenic studies gave rise to diversified and optimized variants of fluorescent proteins, which have never been encountered in nature. This article gives an overview of the GFP-like proteins developed to date and their most common applications to study living specimens using fluorescence microscopy.

Authors+Show Affiliations

Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

16269193

Citation

Chudakov, Dmitriy M., et al. "Fluorescent Proteins as a Toolkit for in Vivo Imaging." Trends in Biotechnology, vol. 23, no. 12, 2005, pp. 605-13.
Chudakov DM, Lukyanov S, Lukyanov KA. Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotechnol. 2005;23(12):605-13.
Chudakov, D. M., Lukyanov, S., & Lukyanov, K. A. (2005). Fluorescent proteins as a toolkit for in vivo imaging. Trends in Biotechnology, 23(12), 605-13.
Chudakov DM, Lukyanov S, Lukyanov KA. Fluorescent Proteins as a Toolkit for in Vivo Imaging. Trends Biotechnol. 2005;23(12):605-13. PubMed PMID: 16269193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorescent proteins as a toolkit for in vivo imaging. AU - Chudakov,Dmitriy M, AU - Lukyanov,Sergey, AU - Lukyanov,Konstantin A, Y1 - 2005/11/02/ PY - 2005/04/28/received PY - 2005/07/21/revised PY - 2005/10/12/accepted PY - 2005/11/5/pubmed PY - 2006/1/19/medline PY - 2005/11/5/entrez SP - 605 EP - 13 JF - Trends in biotechnology JO - Trends Biotechnol. VL - 23 IS - 12 N2 - Green fluorescent protein (GFP) from the jellyfish Aequorea victoria, and its mutant variants, are the only fully genetically encoded fluorescent probes available and they have proved to be excellent tools for labeling living specimens. Since 1999, numerous GFP homologues have been discovered in Anthozoa, Hydrozoa and Copepoda species, demonstrating the broad evolutionary and spectral diversity of this protein family. Mutagenic studies gave rise to diversified and optimized variants of fluorescent proteins, which have never been encountered in nature. This article gives an overview of the GFP-like proteins developed to date and their most common applications to study living specimens using fluorescence microscopy. SN - 0167-7799 UR - https://www.unboundmedicine.com/medline/citation/16269193/Fluorescent_proteins_as_a_toolkit_for_in_vivo_imaging_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-7799(05)00273-8 DB - PRIME DP - Unbound Medicine ER -