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Separation and determination of the effective components in the alabastrum of Edgeworthia chrysantha Lindl. by micellar electrokinetic capillary chromatography.
J Pharm Biomed Anal. 2006 Mar 18; 40(5):1137-42.JP

Abstract

A rapid and efficient micellar electrokinetic capillary chromatography (MEKC) method was developed to analyze edgeworoside C (1), kaempferol-3-O-beta-D-glucoside (2) and rutin (3) in the alabastrum of Edgeworthia chrysantha Lindl. for the first time. The factors that affect the separation were studied, such as the concentrations of the buffer, SDS, and organic modifier, the apparent pH, the applied voltage and temperature. The analytes were well separated within 15 min with an electrolyte containing 25 mM Na(2)B(4)O(7), 30 mM NaH(2)PO(4), 60mM SDS and 15% acetonitrile (pH(*) 9.1) at 25 kV and 15 degrees C. The correlation coefficients between the peak areas of analytes and the corresponding concentrations were 0.9976-0.9981 under the optimum conditions. The relative standard deviations (R.S.D.) of the migration time and peak area were in the range 0.6-1.7 and 1.9-5.3%, respectively. The contents of analytes in E. chrysantha Lindl. collected from the different places were successfully determined with the recoveries ranging from 95.9 to 104.3%. And, the results demonstrated that there was significant difference between the two real samples.

Authors+Show Affiliations

Wang S
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China.
Cheng Y
No affiliation info available

MeSH

BuffersChromatography, Micellar Electrokinetic CapillaryFlowersHydrogen-Ion ConcentrationIndicators and ReagentsReference StandardsReproducibility of ResultsSodium Dodecyl SulfateSolutionsTemperatureThymelaeaceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16269220

Citation

Wang, Shufang, and Yiyu Cheng. "Separation and Determination of the Effective Components in the Alabastrum of Edgeworthia Chrysantha Lindl. By Micellar Electrokinetic Capillary Chromatography." Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 5, 2006, pp. 1137-42.
Wang S, Cheng Y. Separation and determination of the effective components in the alabastrum of Edgeworthia chrysantha Lindl. by micellar electrokinetic capillary chromatography. J Pharm Biomed Anal. 2006;40(5):1137-42.
Wang, S., & Cheng, Y. (2006). Separation and determination of the effective components in the alabastrum of Edgeworthia chrysantha Lindl. by micellar electrokinetic capillary chromatography. Journal of Pharmaceutical and Biomedical Analysis, 40(5), 1137-42.
Wang S, Cheng Y. Separation and Determination of the Effective Components in the Alabastrum of Edgeworthia Chrysantha Lindl. By Micellar Electrokinetic Capillary Chromatography. J Pharm Biomed Anal. 2006 Mar 18;40(5):1137-42. PubMed PMID: 16269220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of the effective components in the alabastrum of Edgeworthia chrysantha Lindl. by micellar electrokinetic capillary chromatography. AU - Wang,Shufang, AU - Cheng,Yiyu, Y1 - 2005/11/02/ PY - 2005/05/28/received PY - 2005/09/06/revised PY - 2005/09/08/accepted PY - 2005/11/5/pubmed PY - 2006/6/6/medline PY - 2005/11/5/entrez SP - 1137 EP - 42 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 40 IS - 5 N2 - A rapid and efficient micellar electrokinetic capillary chromatography (MEKC) method was developed to analyze edgeworoside C (1), kaempferol-3-O-beta-D-glucoside (2) and rutin (3) in the alabastrum of Edgeworthia chrysantha Lindl. for the first time. The factors that affect the separation were studied, such as the concentrations of the buffer, SDS, and organic modifier, the apparent pH, the applied voltage and temperature. The analytes were well separated within 15 min with an electrolyte containing 25 mM Na(2)B(4)O(7), 30 mM NaH(2)PO(4), 60mM SDS and 15% acetonitrile (pH(*) 9.1) at 25 kV and 15 degrees C. The correlation coefficients between the peak areas of analytes and the corresponding concentrations were 0.9976-0.9981 under the optimum conditions. The relative standard deviations (R.S.D.) of the migration time and peak area were in the range 0.6-1.7 and 1.9-5.3%, respectively. The contents of analytes in E. chrysantha Lindl. collected from the different places were successfully determined with the recoveries ranging from 95.9 to 104.3%. And, the results demonstrated that there was significant difference between the two real samples. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16269220/Separation_and_determination_of_the_effective_components_in_the_alabastrum_of_Edgeworthia_chrysantha_Lindl__by_micellar_electrokinetic_capillary_chromatography_ DB - PRIME DP - Unbound Medicine ER -