Tags

Type your tag names separated by a space and hit enter

Comparison of minisatellite polymorphisms in the Bacillus cereus complex: a simple assay for large-scale screening and identification of strains most closely related to Bacillus anthracis.
Appl Environ Microbiol 2005; 71(11):6613-23AE

Abstract

Polymorphism of five tandem repeats that are monomorphic in Bacillus anthracis was investigated in 230 isolates of the B. cereus group and in 5 sequenced B. cereus genomes in search for markers allowing identification of B. cereus and B. thuringiensis strains most closely related to B. anthracis. Using this multiple-locus variable number of tandem repeat analysis (MLVA), a cluster of 30 strains was selected for further characterization. Eventually, six of these were characterized by multilocus sequence type analysis. One of the strains is only six point mutations (of almost 3,000 bp) away from B. anthracis and was also proposed to be closest to B. anthracis by MLVA analysis. However, this strain remains separated from B. anthracis by a number of significant genetic events observed in B. anthracis, including the loss of the hemolysin activity, the presence of four prophages, and the presence of the two virulence plasmids, pXO1 and pXO2. One particular minisatellite marker provides an efficient assay to identify the subset of B. cereus and B. thuringiensis strains closely related to B. anthracis. Based on these results, a very simple assay is proposed that allows the screening of hundreds of strains from the B. cereus complex, with modest equipment and at a low cost, to eventually fill the gap with B. anthracis and better understand the origin and making of this dangerous pathogen.

Authors+Show Affiliations

Institut de Génétique et Microbiologie, Université Paris XI, Orsay, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16269689

Citation

Valjevac, Samina, et al. "Comparison of Minisatellite Polymorphisms in the Bacillus Cereus Complex: a Simple Assay for Large-scale Screening and Identification of Strains Most Closely Related to Bacillus Anthracis." Applied and Environmental Microbiology, vol. 71, no. 11, 2005, pp. 6613-23.
Valjevac S, Hilaire V, Lisanti O, et al. Comparison of minisatellite polymorphisms in the Bacillus cereus complex: a simple assay for large-scale screening and identification of strains most closely related to Bacillus anthracis. Appl Environ Microbiol. 2005;71(11):6613-23.
Valjevac, S., Hilaire, V., Lisanti, O., Ramisse, F., Hernandez, E., Cavallo, J. D., ... Vergnaud, G. (2005). Comparison of minisatellite polymorphisms in the Bacillus cereus complex: a simple assay for large-scale screening and identification of strains most closely related to Bacillus anthracis. Applied and Environmental Microbiology, 71(11), pp. 6613-23.
Valjevac S, et al. Comparison of Minisatellite Polymorphisms in the Bacillus Cereus Complex: a Simple Assay for Large-scale Screening and Identification of Strains Most Closely Related to Bacillus Anthracis. Appl Environ Microbiol. 2005;71(11):6613-23. PubMed PMID: 16269689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of minisatellite polymorphisms in the Bacillus cereus complex: a simple assay for large-scale screening and identification of strains most closely related to Bacillus anthracis. AU - Valjevac,Samina, AU - Hilaire,Valérie, AU - Lisanti,Olivier, AU - Ramisse,Françoise, AU - Hernandez,Eric, AU - Cavallo,Jean-Didier, AU - Pourcel,Christine, AU - Vergnaud,Gilles, PY - 2005/11/5/pubmed PY - 2005/12/21/medline PY - 2005/11/5/entrez SP - 6613 EP - 23 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 71 IS - 11 N2 - Polymorphism of five tandem repeats that are monomorphic in Bacillus anthracis was investigated in 230 isolates of the B. cereus group and in 5 sequenced B. cereus genomes in search for markers allowing identification of B. cereus and B. thuringiensis strains most closely related to B. anthracis. Using this multiple-locus variable number of tandem repeat analysis (MLVA), a cluster of 30 strains was selected for further characterization. Eventually, six of these were characterized by multilocus sequence type analysis. One of the strains is only six point mutations (of almost 3,000 bp) away from B. anthracis and was also proposed to be closest to B. anthracis by MLVA analysis. However, this strain remains separated from B. anthracis by a number of significant genetic events observed in B. anthracis, including the loss of the hemolysin activity, the presence of four prophages, and the presence of the two virulence plasmids, pXO1 and pXO2. One particular minisatellite marker provides an efficient assay to identify the subset of B. cereus and B. thuringiensis strains closely related to B. anthracis. Based on these results, a very simple assay is proposed that allows the screening of hundreds of strains from the B. cereus complex, with modest equipment and at a low cost, to eventually fill the gap with B. anthracis and better understand the origin and making of this dangerous pathogen. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16269689/Comparison_of_minisatellite_polymorphisms_in_the_Bacillus_cereus_complex:_a_simple_assay_for_large_scale_screening_and_identification_of_strains_most_closely_related_to_Bacillus_anthracis_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=16269689 DB - PRIME DP - Unbound Medicine ER -