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Monitoring stress-related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making.
Appl Environ Microbiol. 2005 Nov; 71(11):6831-7.AE

Abstract

Physiological capabilities and fermentation performance of Saccharomyces cerevisiae strains to be employed during industrial wine fermentations are critical for the quality of the final product. During the process of biomass propagation, yeast cells are dynamically exposed to a mixed and interrelated group of known stresses such as osmotic, oxidative, thermic, and/or starvation. These stressing conditions can dramatically affect the parameters of the fermentation process and the technological abilities of the yeast, e.g., the biomass yield and its fermentative capacity. Although a good knowledge exists of the behavior of S. cerevisiae under laboratory conditions, insufficient knowledge is available about yeast stress responses under the specific media and growth conditions during industrial processes. We performed growth experiments using bench-top fermentors and employed a molecular marker approach (changes in expression levels of five stress-related genes) to investigate how the cells respond to environmental changes during the process of yeast biomass production. The data show that in addition to the general stress response pathway, using the HSP12 gene as a marker, other specific stress response pathways were induced, as indicated by the changes detected in the mRNA levels of two stress-related genes, GPD1 and TRX2. These results suggest that the cells were affected by osmotic and oxidative stresses, demonstrating that these are the major causes of the stress response throughout the process of wine yeast biomass production.

Authors+Show Affiliations

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apartado 73, E-46100 Burjassot, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16269716

Citation

Pérez-Torrado, Roberto, et al. "Monitoring Stress-related Genes During the Process of Biomass Propagation of Saccharomyces Cerevisiae Strains Used for Wine Making." Applied and Environmental Microbiology, vol. 71, no. 11, 2005, pp. 6831-7.
Pérez-Torrado R, Bruno-Bárcena JM, Matallana E. Monitoring stress-related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making. Appl Environ Microbiol. 2005;71(11):6831-7.
Pérez-Torrado, R., Bruno-Bárcena, J. M., & Matallana, E. (2005). Monitoring stress-related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making. Applied and Environmental Microbiology, 71(11), 6831-7.
Pérez-Torrado R, Bruno-Bárcena JM, Matallana E. Monitoring Stress-related Genes During the Process of Biomass Propagation of Saccharomyces Cerevisiae Strains Used for Wine Making. Appl Environ Microbiol. 2005;71(11):6831-7. PubMed PMID: 16269716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring stress-related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making. AU - Pérez-Torrado,Roberto, AU - Bruno-Bárcena,Jose M, AU - Matallana,Emilia, PY - 2005/11/5/pubmed PY - 2005/12/21/medline PY - 2005/11/5/entrez SP - 6831 EP - 7 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 71 IS - 11 N2 - Physiological capabilities and fermentation performance of Saccharomyces cerevisiae strains to be employed during industrial wine fermentations are critical for the quality of the final product. During the process of biomass propagation, yeast cells are dynamically exposed to a mixed and interrelated group of known stresses such as osmotic, oxidative, thermic, and/or starvation. These stressing conditions can dramatically affect the parameters of the fermentation process and the technological abilities of the yeast, e.g., the biomass yield and its fermentative capacity. Although a good knowledge exists of the behavior of S. cerevisiae under laboratory conditions, insufficient knowledge is available about yeast stress responses under the specific media and growth conditions during industrial processes. We performed growth experiments using bench-top fermentors and employed a molecular marker approach (changes in expression levels of five stress-related genes) to investigate how the cells respond to environmental changes during the process of yeast biomass production. The data show that in addition to the general stress response pathway, using the HSP12 gene as a marker, other specific stress response pathways were induced, as indicated by the changes detected in the mRNA levels of two stress-related genes, GPD1 and TRX2. These results suggest that the cells were affected by osmotic and oxidative stresses, demonstrating that these are the major causes of the stress response throughout the process of wine yeast biomass production. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16269716/Monitoring_stress_related_genes_during_the_process_of_biomass_propagation_of_Saccharomyces_cerevisiae_strains_used_for_wine_making_ L2 - https://journals.asm.org/doi/10.1128/AEM.71.11.6831-6837.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -