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Determination of three bioactive constituents in moutan cortex by capillary electrophoresis with electrochemical detection.
Anal Sci. 2005 Oct; 21(10):1161-5.AS

Abstract

Capillary electrophoresis with electrochemical detection has been employed for the separation and determination of the three active constituents (paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin) in traditional Chinese medicine, Moutan Cortex (root cortex of Paeonia suffruticosa Andrews). The effects of several important factors, such as the concentration of running buffer, the separation voltage, the injection time, and the detection potential, were investigated to determine the optimum conditions. The detection electrode was a 300 microm diameter carbon-disc electrode at a working potential of +0.90 V (versus SCE). The three analytes could be well separated within 7 min in a 40 cm length fused-silica capillary at a separation voltage of 12 kV in a 50 mM borate buffer (pH 9.2). The relation between the peak current and the analyte concentration was linear over 3 orders of magnitude with detection limits (S/N = 3) ranging from 0.4 to 0.7 microM for all analytes. The proposed method has been successfully applied to the determination of paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin in real plant samples with satisfactory assay results.

Authors+Show Affiliations

Chen G
Department of Chemistry, Fudan University, Shanghai 200032, China. gangchen@fudan.edu.cn
Zhang L
No affiliation info available
Yang P
No affiliation info available

MeSH

AcetophenonesDrugs, Chinese HerbalElectrochemistryElectrophoresis, CapillaryGlycosidesPaeoniaTerpenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16270571

Citation

Chen, Gang, et al. "Determination of Three Bioactive Constituents in Moutan Cortex By Capillary Electrophoresis With Electrochemical Detection." Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry, vol. 21, no. 10, 2005, pp. 1161-5.
Chen G, Zhang L, Yang P. Determination of three bioactive constituents in moutan cortex by capillary electrophoresis with electrochemical detection. Anal Sci. 2005;21(10):1161-5.
Chen, G., Zhang, L., & Yang, P. (2005). Determination of three bioactive constituents in moutan cortex by capillary electrophoresis with electrochemical detection. Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry, 21(10), 1161-5.
Chen G, Zhang L, Yang P. Determination of Three Bioactive Constituents in Moutan Cortex By Capillary Electrophoresis With Electrochemical Detection. Anal Sci. 2005;21(10):1161-5. PubMed PMID: 16270571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of three bioactive constituents in moutan cortex by capillary electrophoresis with electrochemical detection. AU - Chen,Gang, AU - Zhang,Luyan, AU - Yang,Pengyuan, PY - 2005/11/8/pubmed PY - 2006/1/19/medline PY - 2005/11/8/entrez SP - 1161 EP - 5 JF - Analytical sciences : the international journal of the Japan Society for Analytical Chemistry JO - Anal Sci VL - 21 IS - 10 N2 - Capillary electrophoresis with electrochemical detection has been employed for the separation and determination of the three active constituents (paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin) in traditional Chinese medicine, Moutan Cortex (root cortex of Paeonia suffruticosa Andrews). The effects of several important factors, such as the concentration of running buffer, the separation voltage, the injection time, and the detection potential, were investigated to determine the optimum conditions. The detection electrode was a 300 microm diameter carbon-disc electrode at a working potential of +0.90 V (versus SCE). The three analytes could be well separated within 7 min in a 40 cm length fused-silica capillary at a separation voltage of 12 kV in a 50 mM borate buffer (pH 9.2). The relation between the peak current and the analyte concentration was linear over 3 orders of magnitude with detection limits (S/N = 3) ranging from 0.4 to 0.7 microM for all analytes. The proposed method has been successfully applied to the determination of paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin in real plant samples with satisfactory assay results. SN - 0910-6340 UR - https://www.unboundmedicine.com/medline/citation/16270571/Determination_of_three_bioactive_constituents_in_moutan_cortex_by_capillary_electrophoresis_with_electrochemical_detection_ DB - PRIME DP - Unbound Medicine ER -