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Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumor necrosis factor-alpha in patients with chronic and aggressive periodontitis.
J Periodontol 2005; 76(11):1849-55JP

Abstract

BACKGROUND

Monocyte chemoattractant protein-1 (MCP-1) is a well-known chemotactic cytokine that regulates mononuclear inflammatory cell recruitment. This recruitment has particular importance in the oral cavity because inflammatory cells will be challenged with periodontopathogenic bacteria during infections. Tumor necrosis factor-alpha (TNF-alpha) is a cytokine that induces bone resorption by stimulating proliferation and differentiation of osteoclasts' progenitors and also stimulates MCP-1 expression. The aims of this study were to investigate the presence of MCP-1 in gingival crevicular fluid (GCF) samples from patients with chronic periodontitis (CP) and aggressive periodontitis (AgP) and to examine the possible correlations between the GCF levels of MCP-1 and TNF-alpha.

METHODS

Twenty-five CP, 20 AgP, and 20 healthy control (C) patients were selected for the study. Plaque index (PI), gingival index (GI), probing depth (PD), and clinical attachment level (CAL) measurements were recorded from each sampling area. Assays for GCF MCP-1 and TNF-alpha were carried out by an enzyme-linked immunosorbent assay (ELISA) method.

RESULTS

The concentration (nanograms per microliters) and total MCP-1 and TNF-alpha (nanograms per site) were not statistically significant between CP and AgP groups, but total MCP-1 and TNF-alpha was statistically different between CP and C and between AgP and C groups (P <0.001). All clinical parameters were statistically different between CP and C and between AgP and C groups (P <0.001). A positive statistical correlation was detected between the levels of MCP-1 and TNF-alpha, and there was also a positive correlation between all clinical parameters and total MCP-1 and TNF-alpha levels.

CONCLUSIONS

These results suggest that MCP-1 could have an important role in the activation and recruitment of inflammatory and immune cells in periodontal diseases, and both AgP and CP patients may have the same pattern of MCP-1 expression. A strong positive correlation between the GCF levels of MCP-1 and TNF-alpha may account for the mechanism of amplification of inflammatory events in gingival inflammation.

Authors+Show Affiliations

Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey. bulkurtis@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16274303

Citation

Kurtiş, Bülent, et al. "Gingival Crevicular Fluid Levels of Monocyte Chemoattractant Protein-1 and Tumor Necrosis Factor-alpha in Patients With Chronic and Aggressive Periodontitis." Journal of Periodontology, vol. 76, no. 11, 2005, pp. 1849-55.
Kurtiş B, Tüter G, Serdar M, et al. Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumor necrosis factor-alpha in patients with chronic and aggressive periodontitis. J Periodontol. 2005;76(11):1849-55.
Kurtiş, B., Tüter, G., Serdar, M., Akdemir, P., Uygur, C., Firatli, E., & Bal, B. (2005). Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumor necrosis factor-alpha in patients with chronic and aggressive periodontitis. Journal of Periodontology, 76(11), pp. 1849-55.
Kurtiş B, et al. Gingival Crevicular Fluid Levels of Monocyte Chemoattractant Protein-1 and Tumor Necrosis Factor-alpha in Patients With Chronic and Aggressive Periodontitis. J Periodontol. 2005;76(11):1849-55. PubMed PMID: 16274303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumor necrosis factor-alpha in patients with chronic and aggressive periodontitis. AU - Kurtiş,Bülent, AU - Tüter,Gülay, AU - Serdar,Muhittin, AU - Akdemir,Pelin, AU - Uygur,Cem, AU - Firatli,Erhan, AU - Bal,Belgin, PY - 2005/11/9/pubmed PY - 2006/2/8/medline PY - 2005/11/9/entrez SP - 1849 EP - 55 JF - Journal of periodontology JO - J. Periodontol. VL - 76 IS - 11 N2 - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is a well-known chemotactic cytokine that regulates mononuclear inflammatory cell recruitment. This recruitment has particular importance in the oral cavity because inflammatory cells will be challenged with periodontopathogenic bacteria during infections. Tumor necrosis factor-alpha (TNF-alpha) is a cytokine that induces bone resorption by stimulating proliferation and differentiation of osteoclasts' progenitors and also stimulates MCP-1 expression. The aims of this study were to investigate the presence of MCP-1 in gingival crevicular fluid (GCF) samples from patients with chronic periodontitis (CP) and aggressive periodontitis (AgP) and to examine the possible correlations between the GCF levels of MCP-1 and TNF-alpha. METHODS: Twenty-five CP, 20 AgP, and 20 healthy control (C) patients were selected for the study. Plaque index (PI), gingival index (GI), probing depth (PD), and clinical attachment level (CAL) measurements were recorded from each sampling area. Assays for GCF MCP-1 and TNF-alpha were carried out by an enzyme-linked immunosorbent assay (ELISA) method. RESULTS: The concentration (nanograms per microliters) and total MCP-1 and TNF-alpha (nanograms per site) were not statistically significant between CP and AgP groups, but total MCP-1 and TNF-alpha was statistically different between CP and C and between AgP and C groups (P <0.001). All clinical parameters were statistically different between CP and C and between AgP and C groups (P <0.001). A positive statistical correlation was detected between the levels of MCP-1 and TNF-alpha, and there was also a positive correlation between all clinical parameters and total MCP-1 and TNF-alpha levels. CONCLUSIONS: These results suggest that MCP-1 could have an important role in the activation and recruitment of inflammatory and immune cells in periodontal diseases, and both AgP and CP patients may have the same pattern of MCP-1 expression. A strong positive correlation between the GCF levels of MCP-1 and TNF-alpha may account for the mechanism of amplification of inflammatory events in gingival inflammation. SN - 0022-3492 UR - https://www.unboundmedicine.com/medline/citation/16274303/Gingival_crevicular_fluid_levels_of_monocyte_chemoattractant_protein_1_and_tumor_necrosis_factor_alpha_in_patients_with_chronic_and_aggressive_periodontitis_ L2 - https://doi.org/10.1902/jop.2005.76.11.1849 DB - PRIME DP - Unbound Medicine ER -