Tags

Type your tag names separated by a space and hit enter

Cationic liposomes induce apoptosis through p38 MAP kinase-caspase-8-Bid pathway in macrophage-like RAW264.7 cells.
J Leukoc Biol. 2006 Jan; 79(1):184-91.JL

Abstract

We have demonstrated that cationic liposomes composed of stearylamine (SA-liposomes) induce apoptosis in a variety of cells, but the mechanism responsible for the cellular death is not clear. In this paper, we investigated the signaling pathways implicated in SA-liposome-induced apoptosis in the macrophage-like cell line RAW264.7. Treatment with SA-liposomes caused the activation of mitogen-activated protein kinases (MAPKs), especially p38 and c-jun N-terminal kinase, and apoptosis was only inhibited upon the addition of a specific inhibitor for p38. N-acetylcysteine, a scavenger of reactive oxygen species (ROS), effectively inhibited the activation of p38 and cellular death, indicating that the activation induced by ROS is an initial step in the process of apoptosis triggered by SA-liposomes. Caspase-8 was activated by p38, and caspase-8-dependent cleavage of Bid was also observed. No down-regulation of bcl-2 expression, and no cleavage of Bax protein were observed. Taken together, our results suggest that apoptosis of RAW264.7 by SA-liposomes was mediated by the MAPK p38 and a caspase-8-dependent Bid-cleavage pathway. Moreover, we found that ROS can contribute intimately to the SA-liposome-induced cell death in RAW264.7.

Authors+Show Affiliations

School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16275897

Citation

Iwaoka, Sayaka, et al. "Cationic Liposomes Induce Apoptosis Through P38 MAP kinase-caspase-8-Bid Pathway in Macrophage-like RAW264.7 Cells." Journal of Leukocyte Biology, vol. 79, no. 1, 2006, pp. 184-91.
Iwaoka S, Nakamura T, Takano S, et al. Cationic liposomes induce apoptosis through p38 MAP kinase-caspase-8-Bid pathway in macrophage-like RAW264.7 cells. J Leukoc Biol. 2006;79(1):184-91.
Iwaoka, S., Nakamura, T., Takano, S., Tsuchiya, S., & Aramaki, Y. (2006). Cationic liposomes induce apoptosis through p38 MAP kinase-caspase-8-Bid pathway in macrophage-like RAW264.7 cells. Journal of Leukocyte Biology, 79(1), 184-91.
Iwaoka S, et al. Cationic Liposomes Induce Apoptosis Through P38 MAP kinase-caspase-8-Bid Pathway in Macrophage-like RAW264.7 Cells. J Leukoc Biol. 2006;79(1):184-91. PubMed PMID: 16275897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cationic liposomes induce apoptosis through p38 MAP kinase-caspase-8-Bid pathway in macrophage-like RAW264.7 cells. AU - Iwaoka,Sayaka, AU - Nakamura,Tomoko, AU - Takano,Shuhei, AU - Tsuchiya,Seishi, AU - Aramaki,Yukihiko, Y1 - 2005/11/07/ PY - 2005/11/9/pubmed PY - 2006/3/3/medline PY - 2005/11/9/entrez SP - 184 EP - 91 JF - Journal of leukocyte biology JO - J Leukoc Biol VL - 79 IS - 1 N2 - We have demonstrated that cationic liposomes composed of stearylamine (SA-liposomes) induce apoptosis in a variety of cells, but the mechanism responsible for the cellular death is not clear. In this paper, we investigated the signaling pathways implicated in SA-liposome-induced apoptosis in the macrophage-like cell line RAW264.7. Treatment with SA-liposomes caused the activation of mitogen-activated protein kinases (MAPKs), especially p38 and c-jun N-terminal kinase, and apoptosis was only inhibited upon the addition of a specific inhibitor for p38. N-acetylcysteine, a scavenger of reactive oxygen species (ROS), effectively inhibited the activation of p38 and cellular death, indicating that the activation induced by ROS is an initial step in the process of apoptosis triggered by SA-liposomes. Caspase-8 was activated by p38, and caspase-8-dependent cleavage of Bid was also observed. No down-regulation of bcl-2 expression, and no cleavage of Bax protein were observed. Taken together, our results suggest that apoptosis of RAW264.7 by SA-liposomes was mediated by the MAPK p38 and a caspase-8-dependent Bid-cleavage pathway. Moreover, we found that ROS can contribute intimately to the SA-liposome-induced cell death in RAW264.7. SN - 0741-5400 UR - https://www.unboundmedicine.com/medline/citation/16275897/Cationic_liposomes_induce_apoptosis_through_p38_MAP_kinase_caspase_8_Bid_pathway_in_macrophage_like_RAW264_7_cells_ L2 - https://doi.org/10.1189/jlb.0405181 DB - PRIME DP - Unbound Medicine ER -