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Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63.
Clin Diagn Lab Immunol. 2005 Nov; 12(11):1317-21.CD

Abstract

The serological response profile of severe acute respiratory syndrome (SARS) coronavirus (CoV) infection was defined by neutralization tests and subclass-specific immunofluorescent (IF) tests using serial sera from 20 patients. SARS CoV total immunoglobulin (Ig) (IgG, IgA, and IgM [IgGAM]) was the first antibody to be detectable. There was no difference in time to seroconversion between the patients who survived (n = 14) and those who died (n = 6). Although SARS CoV IgM was still detectable by IF tests with 8 of 11 patients at 7 months postinfection, the geometric mean titers dropped from 282 at 1 month postinfection to 19 at 7 months (P = 0.001). In contrast, neutralizing antibody and SARS CoV IgGAM and IgG antibody titers remained stable over this period. The SARS CoV antibody response was sometimes associated with an increase in preexisting IF IgG antibody titers for human coronaviruses OC43, 229E, and NL63. There was no change in IF IgG titer for virus capsid antigen from the herpesvirus that was used as an unrelated control, Epstein-Barr virus. In contrast, patients who had OC43 infections, and probably also 229E infections, without prior exposure to SARS CoV had increases of antibodies specific for the infecting virus but not for SARS CoV. There is a need for awareness of cross-reactive antibody responses between coronaviruses when interpreting IF serology.

Authors+Show Affiliations

Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, University Pathology Building, Pokfulam Rd., Hong Kong, SAR, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16275947

Citation

Chan, K H., et al. "Serological Responses in Patients With Severe Acute Respiratory Syndrome Coronavirus Infection and Cross-reactivity With Human Coronaviruses 229E, OC43, and NL63." Clinical and Diagnostic Laboratory Immunology, vol. 12, no. 11, 2005, pp. 1317-21.
Chan KH, Cheng VC, Woo PC, et al. Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63. Clin Diagn Lab Immunol. 2005;12(11):1317-21.
Chan, K. H., Cheng, V. C., Woo, P. C., Lau, S. K., Poon, L. L., Guan, Y., Seto, W. H., Yuen, K. Y., & Peiris, J. S. (2005). Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63. Clinical and Diagnostic Laboratory Immunology, 12(11), 1317-21.
Chan KH, et al. Serological Responses in Patients With Severe Acute Respiratory Syndrome Coronavirus Infection and Cross-reactivity With Human Coronaviruses 229E, OC43, and NL63. Clin Diagn Lab Immunol. 2005;12(11):1317-21. PubMed PMID: 16275947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63. AU - Chan,K H, AU - Cheng,V C C, AU - Woo,P C Y, AU - Lau,S K P, AU - Poon,L L M, AU - Guan,Y, AU - Seto,W H, AU - Yuen,K Y, AU - Peiris,J S M, PY - 2005/11/9/pubmed PY - 2006/8/29/medline PY - 2005/11/9/entrez SP - 1317 EP - 21 JF - Clinical and diagnostic laboratory immunology JO - Clin Diagn Lab Immunol VL - 12 IS - 11 N2 - The serological response profile of severe acute respiratory syndrome (SARS) coronavirus (CoV) infection was defined by neutralization tests and subclass-specific immunofluorescent (IF) tests using serial sera from 20 patients. SARS CoV total immunoglobulin (Ig) (IgG, IgA, and IgM [IgGAM]) was the first antibody to be detectable. There was no difference in time to seroconversion between the patients who survived (n = 14) and those who died (n = 6). Although SARS CoV IgM was still detectable by IF tests with 8 of 11 patients at 7 months postinfection, the geometric mean titers dropped from 282 at 1 month postinfection to 19 at 7 months (P = 0.001). In contrast, neutralizing antibody and SARS CoV IgGAM and IgG antibody titers remained stable over this period. The SARS CoV antibody response was sometimes associated with an increase in preexisting IF IgG antibody titers for human coronaviruses OC43, 229E, and NL63. There was no change in IF IgG titer for virus capsid antigen from the herpesvirus that was used as an unrelated control, Epstein-Barr virus. In contrast, patients who had OC43 infections, and probably also 229E infections, without prior exposure to SARS CoV had increases of antibodies specific for the infecting virus but not for SARS CoV. There is a need for awareness of cross-reactive antibody responses between coronaviruses when interpreting IF serology. SN - 1071-412X UR - https://www.unboundmedicine.com/medline/citation/16275947/Serological_responses_in_patients_with_severe_acute_respiratory_syndrome_coronavirus_infection_and_cross_reactivity_with_human_coronaviruses_229E_OC43_and_NL63_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=16275947 DB - PRIME DP - Unbound Medicine ER -