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[Construction, genetic stability and protective efficacy of recombinant fowlpox virus expressing hemagglutinin gene of H5N1 subtype avian influenza virus].
Wei Sheng Wu Xue Bao. 2003 Dec; 43(6):722-7.WS

Abstract

Construction, genetic stability and protective efficacy of a recombinant fowlpox virus expressing hemagglutinin (HA) gene (rFPV-HA) of H5 subtype avian influenza virus were conducted. The results indicated that the recombinant fowlpox virus was obtained and purified by blue plaque selection. HA expressed by rFPV-HA was identified by indirect immunofluorescence array. The rFPV-HA could express reporter gene LacZ and HA gene after fifteen successive passages in chicken embryo fibroblast. The rFPV-HA induced completely protection of chickens against lethal challenge with homologous avian influenza virus. The results show that rFPV-HA has good genetic stability and antigenicity and should be a useful tool in HPAI control program by preventing illness and death in chickens when the disease spreads widely.

Authors+Show Affiliations

Jia L
Animal Science and Veterinary Medicine college, Yangzhou University, Yangzhou 225009, China. jialijun2002@yahoo.com.cn
Peng D
No affiliation info available
Zhang Y
No affiliation info available
Liu H
No affiliation info available
Liu X
No affiliation info available

MeSH

AnimalsAntibodies, ViralChick EmbryoChickensFowlpox virusGene ExpressionGenetic EngineeringHemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesInfluenza in BirdsPoultry DiseasesRandom AllocationRecombinant Proteins

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16276892

Citation

Jia, Lijun, et al. "[Construction, Genetic Stability and Protective Efficacy of Recombinant Fowlpox Virus Expressing Hemagglutinin Gene of H5N1 Subtype Avian Influenza Virus]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 43, no. 6, 2003, pp. 722-7.
Jia L, Peng D, Zhang Y, et al. [Construction, genetic stability and protective efficacy of recombinant fowlpox virus expressing hemagglutinin gene of H5N1 subtype avian influenza virus]. Wei Sheng Wu Xue Bao. 2003;43(6):722-7.
Jia, L., Peng, D., Zhang, Y., Liu, H., & Liu, X. (2003). [Construction, genetic stability and protective efficacy of recombinant fowlpox virus expressing hemagglutinin gene of H5N1 subtype avian influenza virus]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 43(6), 722-7.
Jia L, et al. [Construction, Genetic Stability and Protective Efficacy of Recombinant Fowlpox Virus Expressing Hemagglutinin Gene of H5N1 Subtype Avian Influenza Virus]. Wei Sheng Wu Xue Bao. 2003;43(6):722-7. PubMed PMID: 16276892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction, genetic stability and protective efficacy of recombinant fowlpox virus expressing hemagglutinin gene of H5N1 subtype avian influenza virus]. AU - Jia,Lijun, AU - Peng,Daxin, AU - Zhang,Yanmei, AU - Liu,Hongqi, AU - Liu,Xiufan, PY - 2005/11/10/pubmed PY - 2009/4/28/medline PY - 2005/11/10/entrez SP - 722 EP - 7 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 43 IS - 6 N2 - Construction, genetic stability and protective efficacy of a recombinant fowlpox virus expressing hemagglutinin (HA) gene (rFPV-HA) of H5 subtype avian influenza virus were conducted. The results indicated that the recombinant fowlpox virus was obtained and purified by blue plaque selection. HA expressed by rFPV-HA was identified by indirect immunofluorescence array. The rFPV-HA could express reporter gene LacZ and HA gene after fifteen successive passages in chicken embryo fibroblast. The rFPV-HA induced completely protection of chickens against lethal challenge with homologous avian influenza virus. The results show that rFPV-HA has good genetic stability and antigenicity and should be a useful tool in HPAI control program by preventing illness and death in chickens when the disease spreads widely. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/16276892/[Construction_genetic_stability_and_protective_efficacy_of_recombinant_fowlpox_virus_expressing_hemagglutinin_gene_of_H5N1_subtype_avian_influenza_virus]_ DB - PRIME DP - Unbound Medicine ER -