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Inhibition of uropathogenic Escherichia coli by cranberry juice: a new antiadherence assay.
J Agric Food Chem. 2005 Nov 16; 53(23):8940-7.JA

Abstract

A combination of microplate technology and turbidity assessment for testing the adherence of P-fimbriated Escherichia coli to human uroepithelial cell line T24, validated with the addition of the known inhibitor 4-O-alpha-D-galactopyranosyl-alpha-D-galactopyranose (galabiose), resulted in a high-throughput, biologically relevant assessment of cranberry (Vaccinium macrocarpon). P-fimbriated ATCC E. coli strains 25922, 29194, and 49161 were inhibited by galabiose. ATCC 29194, a representative urine isolate containing the papGII allele (Class II fimbrial adhesin) and demonstrating the most significant inhibition in the presence of galabiose, was chosen for further testing. In this assay, a low-polarity fraction of cranberry juice cocktail demonstrated dose-dependent inhibition of E. coli adherence. Reported here, for the first time in V. macrocarpon, are 1-O-methylgalactose, prunin, and phlorizin, identified in an active fraction of cranberry juice concentrate. This in vitro assay will be useful for the standardization of cranberry dietary supplements and is currently being used for bioassay-guided fractionation of cranberry juice concentrate.

Authors+Show Affiliations

UIC/NIH Center for Botanical Dietary Supplements Research for Women's Health, Department of Medicinal Chemistry and Pharmacognosy (MC781), College of Pharmacy, University of Illinois, Chicago, Illinois 60612, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16277386

Citation

Turner, Allison, et al. "Inhibition of Uropathogenic Escherichia Coli By Cranberry Juice: a New Antiadherence Assay." Journal of Agricultural and Food Chemistry, vol. 53, no. 23, 2005, pp. 8940-7.
Turner A, Chen SN, Joike MK, et al. Inhibition of uropathogenic Escherichia coli by cranberry juice: a new antiadherence assay. J Agric Food Chem. 2005;53(23):8940-7.
Turner, A., Chen, S. N., Joike, M. K., Pendland, S. L., Pauli, G. F., & Farnsworth, N. R. (2005). Inhibition of uropathogenic Escherichia coli by cranberry juice: a new antiadherence assay. Journal of Agricultural and Food Chemistry, 53(23), 8940-7.
Turner A, et al. Inhibition of Uropathogenic Escherichia Coli By Cranberry Juice: a New Antiadherence Assay. J Agric Food Chem. 2005 Nov 16;53(23):8940-7. PubMed PMID: 16277386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of uropathogenic Escherichia coli by cranberry juice: a new antiadherence assay. AU - Turner,Allison, AU - Chen,Shao-Nong, AU - Joike,Michele K, AU - Pendland,Susan L, AU - Pauli,Guido F, AU - Farnsworth,Norman R, PY - 2005/11/10/pubmed PY - 2006/1/6/medline PY - 2005/11/10/entrez SP - 8940 EP - 7 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 53 IS - 23 N2 - A combination of microplate technology and turbidity assessment for testing the adherence of P-fimbriated Escherichia coli to human uroepithelial cell line T24, validated with the addition of the known inhibitor 4-O-alpha-D-galactopyranosyl-alpha-D-galactopyranose (galabiose), resulted in a high-throughput, biologically relevant assessment of cranberry (Vaccinium macrocarpon). P-fimbriated ATCC E. coli strains 25922, 29194, and 49161 were inhibited by galabiose. ATCC 29194, a representative urine isolate containing the papGII allele (Class II fimbrial adhesin) and demonstrating the most significant inhibition in the presence of galabiose, was chosen for further testing. In this assay, a low-polarity fraction of cranberry juice cocktail demonstrated dose-dependent inhibition of E. coli adherence. Reported here, for the first time in V. macrocarpon, are 1-O-methylgalactose, prunin, and phlorizin, identified in an active fraction of cranberry juice concentrate. This in vitro assay will be useful for the standardization of cranberry dietary supplements and is currently being used for bioassay-guided fractionation of cranberry juice concentrate. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16277386/Inhibition_of_uropathogenic_Escherichia_coli_by_cranberry_juice:_a_new_antiadherence_assay_ L2 - https://dx.doi.org/10.1021/jf052035u DB - PRIME DP - Unbound Medicine ER -