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Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides).
J Appl Genet. 2005; 46(4):349-55.JA

Abstract

Three low-molecular-weight glutenin subunit (LMW-GS) genes, designated LMW-Td1, LMW-Td2 and LMW-Td3, were isolated from wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of common wheat (T. aestivum). The complete nucleotide sequence lengths of LMW-Td1, LMW-Td2 and LMW-Td3 are 858, 900 and 1062 bp, respectively. LMW-Td1 and LMW-Td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas LMW-Td2 is a putative pseudogene due to the presence of 3 inframe stop codons in its C-terminal domain. The deduced protein sequences of the 3 genes share the same typical polypeptide structures with known LMW-GS genes containing 8 cysteines in the mature protein domains. LMW-Td1 was clearly distinguished from all known LMW-GS genes, and considered as a novel LMW-GS gene. Two hydrophobic motifs (i.e. PIIIL and PVIIL) were observed in the repetitive domain of LMW-Td3. Sequence comparison indicates that sequences of the 3 LMW-GS genes from this study are strongly similar to known LMW-GS genes. Our phylogenetic analysis suggests that LMW-Td1 and LMW-Td2 are homologous with genes on chromosome 1A, and LMW-Td3 is closely related to genes on chromosome 1B.

Authors+Show Affiliations

Yue YW
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China.
Long H
No affiliation info available
Liu Q
No affiliation info available
Wei YM
No affiliation info available
Yan ZH
No affiliation info available
Zheng YL
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceCluster AnalysisDNA PrimersGlutensMolecular Sequence DataPhylogenyProtein Structure, TertiaryProtein SubunitsSequence Analysis, DNATriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16278506

Citation

Yue, Yuan-Wen, et al. "Isolation of Low-molecular-weight Glutenin Subunit Genes From Wild Emmer Wheat (Triticum Dicoccoides)." Journal of Applied Genetics, vol. 46, no. 4, 2005, pp. 349-55.
Yue YW, Long H, Liu Q, et al. Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides). J Appl Genet. 2005;46(4):349-55.
Yue, Y. W., Long, H., Liu, Q., Wei, Y. M., Yan, Z. H., & Zheng, Y. L. (2005). Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides). Journal of Applied Genetics, 46(4), 349-55.
Yue YW, et al. Isolation of Low-molecular-weight Glutenin Subunit Genes From Wild Emmer Wheat (Triticum Dicoccoides). J Appl Genet. 2005;46(4):349-55. PubMed PMID: 16278506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides). AU - Yue,Yuan-Wen, AU - Long,Hai, AU - Liu,Qian, AU - Wei,Yu-Ming, AU - Yan,Ze-Hong, AU - Zheng,You-Liang, PY - 2005/11/10/pubmed PY - 2006/6/13/medline PY - 2005/11/10/entrez SP - 349 EP - 55 JF - Journal of applied genetics JO - J Appl Genet VL - 46 IS - 4 N2 - Three low-molecular-weight glutenin subunit (LMW-GS) genes, designated LMW-Td1, LMW-Td2 and LMW-Td3, were isolated from wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of common wheat (T. aestivum). The complete nucleotide sequence lengths of LMW-Td1, LMW-Td2 and LMW-Td3 are 858, 900 and 1062 bp, respectively. LMW-Td1 and LMW-Td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas LMW-Td2 is a putative pseudogene due to the presence of 3 inframe stop codons in its C-terminal domain. The deduced protein sequences of the 3 genes share the same typical polypeptide structures with known LMW-GS genes containing 8 cysteines in the mature protein domains. LMW-Td1 was clearly distinguished from all known LMW-GS genes, and considered as a novel LMW-GS gene. Two hydrophobic motifs (i.e. PIIIL and PVIIL) were observed in the repetitive domain of LMW-Td3. Sequence comparison indicates that sequences of the 3 LMW-GS genes from this study are strongly similar to known LMW-GS genes. Our phylogenetic analysis suggests that LMW-Td1 and LMW-Td2 are homologous with genes on chromosome 1A, and LMW-Td3 is closely related to genes on chromosome 1B. SN - 1234-1983 UR - https://www.unboundmedicine.com/medline/citation/16278506/Isolation_of_low_molecular_weight_glutenin_subunit_genes_from_wild_emmer_wheat__Triticum_dicoccoides__ DB - PRIME DP - Unbound Medicine ER -