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Increased intracellular pro- and anti-inflammatory cytokines in bronchoalveolar lavage T cells of stable lung transplant patients.
Transplantation. 2005 Oct 27; 80(8):1040-5.T

Abstract

BACKGROUND

Allograft rejection remains a major cause of morbidity and mortality following lung transplantation and is associated with an increased expression of T-cell proinflammatory cytokines. We have recently shown that peripheral blood T-cell proinflammatory cytokine production was significantly reduced in stable lung transplant patients consistent with immunosuppression therapy. However, analysis of inflammatory cytokine profiles in bronchoalveolar lavage (BAL) T cells may be more relevant than peripheral blood T cells for assessing graft status.

METHODS

To investigate the immunomodulatory effects of currently used immunosuppressive regimens on BAL T-cell cytokine production, whole blood and BAL from stable lung transplant patients and control volunteers was stimulated in vitro and cytokine production by CD8+ and CD4+ T-cell subsets determined using multiparameter flow cytometry.

RESULTS

There was a significant decrease in T-cell proinflammatory cytokine production in BAL compared with blood from control subjects but not transplant patients. Anti-inflammatory cytokine IL-4 was increased in BAL compared with blood from both groups. There was a significant increase in IFNgamma, IL-2, IL-4, TGFbeta, and TNFalpha production by CD8 T cells and IFNgamma and TNFalpha production by CD4 T cells in BAL from transplant patients compared with controls.

CONCLUSIONS

We have shown decreased T-cell pro- and anti-inflammatory cytokine production in BAL compared with blood in control subjects but not in stable lung transplant patients. Current immunosuppression protocols have limited effect on T-cell proinflammatory cytokine production in BAL but do upregulate anti-inflammatory cytokines IL-4 and TGFbeta. Drugs that effectively reduce T-cell proinflammatory cytokine production in BAL may improve current protocols for reducing graft rejection in these patients.

Authors+Show Affiliations

Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, Australia. hodgeg@mail.wch.sa.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16278583

Citation

Hodge, Greg, et al. "Increased Intracellular Pro- and Anti-inflammatory Cytokines in Bronchoalveolar Lavage T Cells of Stable Lung Transplant Patients." Transplantation, vol. 80, no. 8, 2005, pp. 1040-5.
Hodge G, Hodge S, Reynolds PN, et al. Increased intracellular pro- and anti-inflammatory cytokines in bronchoalveolar lavage T cells of stable lung transplant patients. Transplantation. 2005;80(8):1040-5.
Hodge, G., Hodge, S., Reynolds, P. N., & Holmes, M. (2005). Increased intracellular pro- and anti-inflammatory cytokines in bronchoalveolar lavage T cells of stable lung transplant patients. Transplantation, 80(8), 1040-5.
Hodge G, et al. Increased Intracellular Pro- and Anti-inflammatory Cytokines in Bronchoalveolar Lavage T Cells of Stable Lung Transplant Patients. Transplantation. 2005 Oct 27;80(8):1040-5. PubMed PMID: 16278583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased intracellular pro- and anti-inflammatory cytokines in bronchoalveolar lavage T cells of stable lung transplant patients. AU - Hodge,Greg, AU - Hodge,Sandra, AU - Reynolds,Paul N, AU - Holmes,Mark, PY - 2005/11/10/pubmed PY - 2005/12/16/medline PY - 2005/11/10/entrez SP - 1040 EP - 5 JF - Transplantation JO - Transplantation VL - 80 IS - 8 N2 - BACKGROUND: Allograft rejection remains a major cause of morbidity and mortality following lung transplantation and is associated with an increased expression of T-cell proinflammatory cytokines. We have recently shown that peripheral blood T-cell proinflammatory cytokine production was significantly reduced in stable lung transplant patients consistent with immunosuppression therapy. However, analysis of inflammatory cytokine profiles in bronchoalveolar lavage (BAL) T cells may be more relevant than peripheral blood T cells for assessing graft status. METHODS: To investigate the immunomodulatory effects of currently used immunosuppressive regimens on BAL T-cell cytokine production, whole blood and BAL from stable lung transplant patients and control volunteers was stimulated in vitro and cytokine production by CD8+ and CD4+ T-cell subsets determined using multiparameter flow cytometry. RESULTS: There was a significant decrease in T-cell proinflammatory cytokine production in BAL compared with blood from control subjects but not transplant patients. Anti-inflammatory cytokine IL-4 was increased in BAL compared with blood from both groups. There was a significant increase in IFNgamma, IL-2, IL-4, TGFbeta, and TNFalpha production by CD8 T cells and IFNgamma and TNFalpha production by CD4 T cells in BAL from transplant patients compared with controls. CONCLUSIONS: We have shown decreased T-cell pro- and anti-inflammatory cytokine production in BAL compared with blood in control subjects but not in stable lung transplant patients. Current immunosuppression protocols have limited effect on T-cell proinflammatory cytokine production in BAL but do upregulate anti-inflammatory cytokines IL-4 and TGFbeta. Drugs that effectively reduce T-cell proinflammatory cytokine production in BAL may improve current protocols for reducing graft rejection in these patients. SN - 0041-1337 UR - https://www.unboundmedicine.com/medline/citation/16278583/Increased_intracellular_pro__and_anti_inflammatory_cytokines_in_bronchoalveolar_lavage_T_cells_of_stable_lung_transplant_patients_ L2 - https://doi.org/10.1097/01.tp.0000173997.92753.25 DB - PRIME DP - Unbound Medicine ER -