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Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease.
J Clin Virol. 2006 Mar; 35(3):292-7.JC

Abstract

BACKGROUND

Human rhinoviruses (HRVs) together with the closely related human enteroviruses (HEVs) cause most of the acute respiratory illnesses throughout the year. HRVs have been detected in most parts of the respiratory tract but not in pharyngeal tonsils.

OBJECTIVES

We aimed to find out whether HRVs were detectable in tonsillar tissue and if their presence correlated to the tonsillar disease.

STUDY DESIGN

Thirty-three tonsillar samples collected in February-March 2003 from children with no acute respiratory symptoms were studied with HRV in situ hybridization (HRV-ISH). Ten tonsillar samples were further examined in a separate laboratory by two different reverse transcription polymerase chain reaction (RT-PCR) methods designed for detection of HRV/HEV RNA.

RESULTS

Twenty of the 33 samples (62%) were positive by HRV-ISH. Five positive and five negative HRV-ISH samples were investigated by two different PCR methods. HRV/HEV RNA was detected in 9 of the 10 specimens by a hanging drop-nested PCR. One HRV-ISH negative sample was positive by a conventional non-nested PCR. One of the samples studied by all three methods, from a patient with recurrent tonsillitis, had no detectable HRV/HEV RNA. Positive result in HRV-ISH did not correlate significantly with underlying tonsillar disease, history of respiratory infections or bronchial asthma. Altogether HRV/HEV RNA was detected in 75% of the tonsils with no correlation to patients' operation indication or history of respiratory diseases.

CONCLUSIONS

In February-March, HRV/HEV RNA was frequently found in tonsillar tissue in children irrespective of the tonsillar pathology. Whether detection of the RNA is a marker of chronic infection or is merely remnant of past infection is not known.

Authors+Show Affiliations

Department of Otorhinolaryngology, Helsinki University Hospital, Lohja Hospital, Lohjantie 26 A, FI-03100 Nummela, Finland. jari.suvilehto@kolumbus.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16280256

Citation

Suvilehto, Jari, et al. "Rhinovirus/enterovirus RNA in Tonsillar Tissue of Children With Tonsillar Disease." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 35, no. 3, 2006, pp. 292-7.
Suvilehto J, Roivainen M, Seppänen M, et al. Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease. J Clin Virol. 2006;35(3):292-7.
Suvilehto, J., Roivainen, M., Seppänen, M., Meri, S., Hovi, T., Carpén, O., & Pitkäranta, A. (2006). Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 35(3), 292-7.
Suvilehto J, et al. Rhinovirus/enterovirus RNA in Tonsillar Tissue of Children With Tonsillar Disease. J Clin Virol. 2006;35(3):292-7. PubMed PMID: 16280256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease. AU - Suvilehto,Jari, AU - Roivainen,Merja, AU - Seppänen,Mikko, AU - Meri,Seppo, AU - Hovi,Tapani, AU - Carpén,Olli, AU - Pitkäranta,Anne, Y1 - 2005/11/08/ PY - 2005/07/20/received PY - 2005/08/19/revised PY - 2005/08/26/accepted PY - 2005/11/11/pubmed PY - 2006/4/28/medline PY - 2005/11/11/entrez SP - 292 EP - 7 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 35 IS - 3 N2 - BACKGROUND: Human rhinoviruses (HRVs) together with the closely related human enteroviruses (HEVs) cause most of the acute respiratory illnesses throughout the year. HRVs have been detected in most parts of the respiratory tract but not in pharyngeal tonsils. OBJECTIVES: We aimed to find out whether HRVs were detectable in tonsillar tissue and if their presence correlated to the tonsillar disease. STUDY DESIGN: Thirty-three tonsillar samples collected in February-March 2003 from children with no acute respiratory symptoms were studied with HRV in situ hybridization (HRV-ISH). Ten tonsillar samples were further examined in a separate laboratory by two different reverse transcription polymerase chain reaction (RT-PCR) methods designed for detection of HRV/HEV RNA. RESULTS: Twenty of the 33 samples (62%) were positive by HRV-ISH. Five positive and five negative HRV-ISH samples were investigated by two different PCR methods. HRV/HEV RNA was detected in 9 of the 10 specimens by a hanging drop-nested PCR. One HRV-ISH negative sample was positive by a conventional non-nested PCR. One of the samples studied by all three methods, from a patient with recurrent tonsillitis, had no detectable HRV/HEV RNA. Positive result in HRV-ISH did not correlate significantly with underlying tonsillar disease, history of respiratory infections or bronchial asthma. Altogether HRV/HEV RNA was detected in 75% of the tonsils with no correlation to patients' operation indication or history of respiratory diseases. CONCLUSIONS: In February-March, HRV/HEV RNA was frequently found in tonsillar tissue in children irrespective of the tonsillar pathology. Whether detection of the RNA is a marker of chronic infection or is merely remnant of past infection is not known. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/16280256/Rhinovirus/enterovirus_RNA_in_tonsillar_tissue_of_children_with_tonsillar_disease_ DB - PRIME DP - Unbound Medicine ER -