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Characterization of low-molecular-weight glutenin subunit genes and their protein products in common wheats.
Theor Appl Genet. 2006 Jan; 112(2):327-34.TA

Abstract

To characterize the low-molecular-weight glutenin subunit (LMW-GS), we developed specific PCR primer sets to distinguish 12 groups of LMW-GS genes of Norin 61 and to decide their loci with nullisomic-tetrasomic lines of Chinese Spring. Three, two, and ten groups were assigned to Glu-A3, Glu-B3, and Glu-D3 loci, respectively. To identify the proteins containing the corresponding amino acid sequences, we determined the N-terminal amino acid sequence of 12 spots of LMW-GSs of Norin 61 separated by two-dimensional gel electrophoresis (2DE). The N-terminal sequences of the LMW-GS spots showed that 10 of 12 groups of LMW-GSs were expressed as protein products, which included LMW-i, LMW-m, and LMW-s types. Four spots were encoded by Glu-A3 (LMW-i). Three spots were encoded by Glu-B3 (LMW-m and LMW-s). Five spots were encoded by Glu-D3 (LMW-m and LMW-s). A minor spot of LMW-m seemed to be encoded by the same Glu-B3 gene as a major spot of LMW-s, but processed at a different site. Comparing among various cultivars, there were polymorphic and non-polymorphic LMW-GSs. Glu-A3 was highly polymorphic, i.e., the a, b, and c alleles showed one spot, the d allele showed four spots, and the e allele had no spot. Insignia used as one of the Glu-A3 null standard cultivars had a LMW-GS encoded by Glu-A3. We also found that Cheyenne had a new Glu-D3 allele. Classification of LMW-GS by a combination of PCR and 2DE will be useful to identify individual LMW-GSs and to study their contribution to flour quality.

Authors+Show Affiliations

Ikeda TM
Department of Crop Breeding, National Agricultural Research Center for Western Region, 6-12-1 Nishifukatsu, Fukuyama 721-8514, Japan. tmikeda@affrc.go.jp
Araki E
No affiliation info available
Fujita Y
No affiliation info available
Yano H
No affiliation info available

MeSH

Amino Acid SequenceElectrophoresis, Gel, Two-DimensionalGlutensMolecular Sequence DataMolecular WeightPolymerase Chain ReactionTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16283233

Citation

Ikeda, T M., et al. "Characterization of Low-molecular-weight Glutenin Subunit Genes and Their Protein Products in Common Wheats." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 112, no. 2, 2006, pp. 327-34.
Ikeda TM, Araki E, Fujita Y, et al. Characterization of low-molecular-weight glutenin subunit genes and their protein products in common wheats. Theor Appl Genet. 2006;112(2):327-34.
Ikeda, T. M., Araki, E., Fujita, Y., & Yano, H. (2006). Characterization of low-molecular-weight glutenin subunit genes and their protein products in common wheats. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 112(2), 327-34.
Ikeda TM, et al. Characterization of Low-molecular-weight Glutenin Subunit Genes and Their Protein Products in Common Wheats. Theor Appl Genet. 2006;112(2):327-34. PubMed PMID: 16283233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of low-molecular-weight glutenin subunit genes and their protein products in common wheats. AU - Ikeda,T M, AU - Araki,E, AU - Fujita,Y, AU - Yano,H, Y1 - 2005/11/11/ PY - 2005/05/30/received PY - 2005/07/19/accepted PY - 2005/11/12/pubmed PY - 2006/3/8/medline PY - 2005/11/12/entrez SP - 327 EP - 34 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 112 IS - 2 N2 - To characterize the low-molecular-weight glutenin subunit (LMW-GS), we developed specific PCR primer sets to distinguish 12 groups of LMW-GS genes of Norin 61 and to decide their loci with nullisomic-tetrasomic lines of Chinese Spring. Three, two, and ten groups were assigned to Glu-A3, Glu-B3, and Glu-D3 loci, respectively. To identify the proteins containing the corresponding amino acid sequences, we determined the N-terminal amino acid sequence of 12 spots of LMW-GSs of Norin 61 separated by two-dimensional gel electrophoresis (2DE). The N-terminal sequences of the LMW-GS spots showed that 10 of 12 groups of LMW-GSs were expressed as protein products, which included LMW-i, LMW-m, and LMW-s types. Four spots were encoded by Glu-A3 (LMW-i). Three spots were encoded by Glu-B3 (LMW-m and LMW-s). Five spots were encoded by Glu-D3 (LMW-m and LMW-s). A minor spot of LMW-m seemed to be encoded by the same Glu-B3 gene as a major spot of LMW-s, but processed at a different site. Comparing among various cultivars, there were polymorphic and non-polymorphic LMW-GSs. Glu-A3 was highly polymorphic, i.e., the a, b, and c alleles showed one spot, the d allele showed four spots, and the e allele had no spot. Insignia used as one of the Glu-A3 null standard cultivars had a LMW-GS encoded by Glu-A3. We also found that Cheyenne had a new Glu-D3 allele. Classification of LMW-GS by a combination of PCR and 2DE will be useful to identify individual LMW-GSs and to study their contribution to flour quality. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16283233/Characterization_of_low_molecular_weight_glutenin_subunit_genes_and_their_protein_products_in_common_wheats_ DB - PRIME DP - Unbound Medicine ER -