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An investigation of the divergence of major ampullate silk fibers from Nephila clavipes and Argiope aurantia.
Biomacromolecules 2005 Nov-Dec; 6(6):3095-9B

Abstract

The major ampullate fiber of both Nephila clavipes and Argiope aurantia is composed of two different proteins, MaSp1 and MaSp2. Each of these proteins has a highly conserved pattern of silk-associated amino acid motifs. The GPGXX motif is the only source of proline and is unique to MaSp2. On the basis of the percent of proline, Nephila clavipes major ampullate silk was calculated to consist of 19% MaSp2 and 81% MaSp1, while Argiope aurantia was calculated to have a significantly higher MaSp2 content of 59% with MaSp1 comprising the remaining 41%. To investigate the functional implications of the difference in protein composition, major ampullate silk fibers from Nephila clavipes and Argiope aurantia were mechanically tested and compared. Stress-strain curves produced from polynomial regression show that the two significant differences between major ampullate silk fibers from Nephila clavipes and Argiope aurantia are the average peak load stress and Young's modulus, with Argiope higher for both.

Authors+Show Affiliations

Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA. abrooks@uwyo.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16283732

Citation

Brooks, Amanda E., et al. "An Investigation of the Divergence of Major Ampullate Silk Fibers From Nephila Clavipes and Argiope Aurantia." Biomacromolecules, vol. 6, no. 6, 2005, pp. 3095-9.
Brooks AE, Steinkraus HB, Nelson SR, et al. An investigation of the divergence of major ampullate silk fibers from Nephila clavipes and Argiope aurantia. Biomacromolecules. 2005;6(6):3095-9.
Brooks, A. E., Steinkraus, H. B., Nelson, S. R., & Lewis, R. V. (2005). An investigation of the divergence of major ampullate silk fibers from Nephila clavipes and Argiope aurantia. Biomacromolecules, 6(6), pp. 3095-9.
Brooks AE, et al. An Investigation of the Divergence of Major Ampullate Silk Fibers From Nephila Clavipes and Argiope Aurantia. Biomacromolecules. 2005;6(6):3095-9. PubMed PMID: 16283732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An investigation of the divergence of major ampullate silk fibers from Nephila clavipes and Argiope aurantia. AU - Brooks,Amanda E, AU - Steinkraus,Holly B, AU - Nelson,Shane R, AU - Lewis,Randolph V, PY - 2005/11/15/pubmed PY - 2006/2/8/medline PY - 2005/11/15/entrez SP - 3095 EP - 9 JF - Biomacromolecules JO - Biomacromolecules VL - 6 IS - 6 N2 - The major ampullate fiber of both Nephila clavipes and Argiope aurantia is composed of two different proteins, MaSp1 and MaSp2. Each of these proteins has a highly conserved pattern of silk-associated amino acid motifs. The GPGXX motif is the only source of proline and is unique to MaSp2. On the basis of the percent of proline, Nephila clavipes major ampullate silk was calculated to consist of 19% MaSp2 and 81% MaSp1, while Argiope aurantia was calculated to have a significantly higher MaSp2 content of 59% with MaSp1 comprising the remaining 41%. To investigate the functional implications of the difference in protein composition, major ampullate silk fibers from Nephila clavipes and Argiope aurantia were mechanically tested and compared. Stress-strain curves produced from polynomial regression show that the two significant differences between major ampullate silk fibers from Nephila clavipes and Argiope aurantia are the average peak load stress and Young's modulus, with Argiope higher for both. SN - 1525-7797 UR - https://www.unboundmedicine.com/medline/citation/16283732/An_investigation_of_the_divergence_of_major_ampullate_silk_fibers_from_Nephila_clavipes_and_Argiope_aurantia_ L2 - https://dx.doi.org/10.1021/bm050421e DB - PRIME DP - Unbound Medicine ER -