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Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk.
Cancer Epidemiol Biomarkers Prev 2005; 14(11 Pt 1):2544-9CE

Abstract

Human papillomavirus (HPV) infection is a high-risk factor for cervical intraepithelial neoplasm (CIN) but the association between the quantitative HPV DNA load and the severity of CIN remains controversial. We conducted a community study to investigate the correlation between the two. Potential study subjects were selected through Pap smear screening in Kaohsiung County, Taiwan. Ninety-one subjects with either their first case of inflammation or > or =CIN1 by biopsy confirmation were assigned to a case group; 175 normal subjects with negative findings by Pap smears or biopsies were assigned to a control group. Cervical HPV load was detected with Hybrid Capture II assay for high-risk HPV infection, with nested PCR for high- and low-risk HPV infection, and with type-specific PCR for HPV type 16 (HPV-16). Individuals with positive high-risk HPV infection had an increased risk of developing CIN. Compared with HPV-negative subjects, the odds ratios were 32.2 [95% confidence interval (95% CI), 10.4-99.5] for subjects with CIN1, 37.2 (95% CI, 7.4-187.6) for subjects with CIN2, and 68.3 (95% CI, 14.1-328.5) for subjects with > or =CIN3 after adjusting for other confounding factors. The similar trend was also found among the HPV-16-negative individuals. In addition, high-risk HPV DNA load levels were highly correlated with the different grades of CINs in the overall population (Spearman's correlation coefficient r = 0.67, P < 0.0001, n = 266) and the HPV-16-negative population (Spearman's correlation coefficient r = 0.58, P < 0.0001, n = 234). We concluded that high-risk HPV infection, irrespective of HPV-16 infection, was highly and positively associated with the development of CIN.

Authors+Show Affiliations

Graduate Institute of Occupational Safety and Health, Kaohsiung Medical University, Building CS 917, 100 Shih-Chuan 1st Road, Kaohsiung 807, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16284376

Citation

Tsai, Hsiu-Ting, et al. "Association Between Quantitative High-risk Human Papillomavirus DNA Load and Cervical Intraepithelial Neoplasm Risk." Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored By the American Society of Preventive Oncology, vol. 14, no. 11 Pt 1, 2005, pp. 2544-9.
Tsai HT, Wu CH, Lai HL, et al. Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk. Cancer Epidemiol Biomarkers Prev. 2005;14(11 Pt 1):2544-9.
Tsai, H. T., Wu, C. H., Lai, H. L., Li, R. N., Tung, Y. C., Chuang, H. Y., ... Wu, M. T. (2005). Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk. Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored By the American Society of Preventive Oncology, 14(11 Pt 1), pp. 2544-9.
Tsai HT, et al. Association Between Quantitative High-risk Human Papillomavirus DNA Load and Cervical Intraepithelial Neoplasm Risk. Cancer Epidemiol Biomarkers Prev. 2005;14(11 Pt 1):2544-9. PubMed PMID: 16284376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk. AU - Tsai,Hsiu-Ting, AU - Wu,Ching-Hu, AU - Lai,Hsiao-Ling, AU - Li,Ruei-Nian, AU - Tung,Yi-Ching, AU - Chuang,Hung-Yi, AU - Wu,Trong-Neng, AU - Lin,Li-Jen, AU - Ho,Chi-Kung, AU - Liu,Hon-Wein, AU - Wu,Ming-Tsang, PY - 2005/11/15/pubmed PY - 2006/1/13/medline PY - 2005/11/15/entrez SP - 2544 EP - 9 JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology JO - Cancer Epidemiol. Biomarkers Prev. VL - 14 IS - 11 Pt 1 N2 - Human papillomavirus (HPV) infection is a high-risk factor for cervical intraepithelial neoplasm (CIN) but the association between the quantitative HPV DNA load and the severity of CIN remains controversial. We conducted a community study to investigate the correlation between the two. Potential study subjects were selected through Pap smear screening in Kaohsiung County, Taiwan. Ninety-one subjects with either their first case of inflammation or > or =CIN1 by biopsy confirmation were assigned to a case group; 175 normal subjects with negative findings by Pap smears or biopsies were assigned to a control group. Cervical HPV load was detected with Hybrid Capture II assay for high-risk HPV infection, with nested PCR for high- and low-risk HPV infection, and with type-specific PCR for HPV type 16 (HPV-16). Individuals with positive high-risk HPV infection had an increased risk of developing CIN. Compared with HPV-negative subjects, the odds ratios were 32.2 [95% confidence interval (95% CI), 10.4-99.5] for subjects with CIN1, 37.2 (95% CI, 7.4-187.6) for subjects with CIN2, and 68.3 (95% CI, 14.1-328.5) for subjects with > or =CIN3 after adjusting for other confounding factors. The similar trend was also found among the HPV-16-negative individuals. In addition, high-risk HPV DNA load levels were highly correlated with the different grades of CINs in the overall population (Spearman's correlation coefficient r = 0.67, P < 0.0001, n = 266) and the HPV-16-negative population (Spearman's correlation coefficient r = 0.58, P < 0.0001, n = 234). We concluded that high-risk HPV infection, irrespective of HPV-16 infection, was highly and positively associated with the development of CIN. SN - 1055-9965 UR - https://www.unboundmedicine.com/medline/citation/16284376/Association_between_quantitative_high_risk_human_papillomavirus_DNA_load_and_cervical_intraepithelial_neoplasm_risk_ L2 - http://cebp.aacrjournals.org/cgi/pmidlookup?view=long&amp;pmid=16284376 DB - PRIME DP - Unbound Medicine ER -