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Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales).
Carbohydr Res. 2005 Dec 30; 340(18):2742-51.CR

Abstract

The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by beta-D-galactose 2-sulfate linked to alpha-D-galactose 2,3,6-trisulfate and beta-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of beta-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2.

Authors+Show Affiliations

Rodríguez MC
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
Merino ER
No affiliation info available
Pujol CA
No affiliation info available
Damonte EB
No affiliation info available
Cerezo AS
No affiliation info available
Matulewicz MC
No affiliation info available

MeSH

Antiviral AgentsCarbon IsotopesGalactansMagnetic Resonance SpectroscopyPlant ExtractsSeaweedSodium HydroxideSpectrophotometry, Infrared

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16289051

Citation

Rodríguez, María C., et al. "Galactans From Cystocarpic Plants of the Red Seaweed Callophyllis Variegata (Kallymeniaceae, Gigartinales)." Carbohydrate Research, vol. 340, no. 18, 2005, pp. 2742-51.
Rodríguez MC, Merino ER, Pujol CA, et al. Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales). Carbohydr Res. 2005;340(18):2742-51.
Rodríguez, M. C., Merino, E. R., Pujol, C. A., Damonte, E. B., Cerezo, A. S., & Matulewicz, M. C. (2005). Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales). Carbohydrate Research, 340(18), 2742-51.
Rodríguez MC, et al. Galactans From Cystocarpic Plants of the Red Seaweed Callophyllis Variegata (Kallymeniaceae, Gigartinales). Carbohydr Res. 2005 Dec 30;340(18):2742-51. PubMed PMID: 16289051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales). AU - Rodríguez,María C, AU - Merino,Emilia R, AU - Pujol,Carlos A, AU - Damonte,Elsa B, AU - Cerezo,Alberto S, AU - Matulewicz,María C, Y1 - 2005/11/09/ PY - 2005/05/20/received PY - 2005/10/04/accepted PY - 2005/11/18/pubmed PY - 2006/3/18/medline PY - 2005/11/18/entrez SP - 2742 EP - 51 JF - Carbohydrate research JO - Carbohydr Res VL - 340 IS - 18 N2 - The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by beta-D-galactose 2-sulfate linked to alpha-D-galactose 2,3,6-trisulfate and beta-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of beta-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/16289051/Galactans_from_cystocarpic_plants_of_the_red_seaweed_Callophyllis_variegata__Kallymeniaceae_Gigartinales__ DB - PRIME DP - Unbound Medicine ER -