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Isolation of iridoid and secoiridoid glycosides and comparative study on Radix gentianae and related adulterants by HPLC analysis.
Phytochemistry. 2005 Nov; 66(22):2674-80.P

Abstract

HPLC profile guided study led to the isolation of an acylated secoiridoid glycoside, named gentiotrifloroside (1), together with six known compounds, i.e., loganic acid (2), 6-O-beta-d-glucopyranosylgentiopicroside (3), swertiamarin (4), gentiopicroside (5), sweroside (6) and 2 -(o,m-dihydroxybenzyl)-sweroside (7) from Gentiana triflora and Gentiana rigescens. The structure of 1 was deduced from one- and two-dimensional NMR spectroscopic experiments. Compounds 1-7 were used successfully as chemical markers for the comparison of the four species of Gentiana used as Radix gentianae. Additionally, differentiation of Gentiana species mentioned and those used as adulterants was evaluated. The close similarity of chemical composition among the four genuine Gentiana species explain their popular usage as R. gentianae in Chinese medicine. We have also shown that the variation of chemical composition in R. gentianae and related adulterants agree well with their botanical phylogeny.

Authors+Show Affiliations

Jiang RW
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Wong KL
No affiliation info available
Chan YM
No affiliation info available
Xu HX
No affiliation info available
But PP
No affiliation info available
Shaw PC
No affiliation info available

MeSH

AnimalsChromatography, High Pressure LiquidGentianaGlycosidesIridoidsMagnetic Resonance SpectroscopyMolecular Structure

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16289148

Citation

Jiang, Ren-Wang, et al. "Isolation of Iridoid and Secoiridoid Glycosides and Comparative Study On Radix Gentianae and Related Adulterants By HPLC Analysis." Phytochemistry, vol. 66, no. 22, 2005, pp. 2674-80.
Jiang RW, Wong KL, Chan YM, et al. Isolation of iridoid and secoiridoid glycosides and comparative study on Radix gentianae and related adulterants by HPLC analysis. Phytochemistry. 2005;66(22):2674-80.
Jiang, R. W., Wong, K. L., Chan, Y. M., Xu, H. X., But, P. P., & Shaw, P. C. (2005). Isolation of iridoid and secoiridoid glycosides and comparative study on Radix gentianae and related adulterants by HPLC analysis. Phytochemistry, 66(22), 2674-80.
Jiang RW, et al. Isolation of Iridoid and Secoiridoid Glycosides and Comparative Study On Radix Gentianae and Related Adulterants By HPLC Analysis. Phytochemistry. 2005;66(22):2674-80. PubMed PMID: 16289148.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of iridoid and secoiridoid glycosides and comparative study on Radix gentianae and related adulterants by HPLC analysis. AU - Jiang,Ren-Wang, AU - Wong,Ka-Lok, AU - Chan,Yiu-Man, AU - Xu,Hong-Xi, AU - But,Paul Pui-Hay, AU - Shaw,Pang-Chui, Y1 - 2005/11/09/ PY - 2005/02/21/received PY - 2005/08/03/revised PY - 2005/11/18/pubmed PY - 2006/4/4/medline PY - 2005/11/18/entrez SP - 2674 EP - 80 JF - Phytochemistry JO - Phytochemistry VL - 66 IS - 22 N2 - HPLC profile guided study led to the isolation of an acylated secoiridoid glycoside, named gentiotrifloroside (1), together with six known compounds, i.e., loganic acid (2), 6-O-beta-d-glucopyranosylgentiopicroside (3), swertiamarin (4), gentiopicroside (5), sweroside (6) and 2 -(o,m-dihydroxybenzyl)-sweroside (7) from Gentiana triflora and Gentiana rigescens. The structure of 1 was deduced from one- and two-dimensional NMR spectroscopic experiments. Compounds 1-7 were used successfully as chemical markers for the comparison of the four species of Gentiana used as Radix gentianae. Additionally, differentiation of Gentiana species mentioned and those used as adulterants was evaluated. The close similarity of chemical composition among the four genuine Gentiana species explain their popular usage as R. gentianae in Chinese medicine. We have also shown that the variation of chemical composition in R. gentianae and related adulterants agree well with their botanical phylogeny. SN - 0031-9422 UR - https://www.unboundmedicine.com/medline/citation/16289148/Isolation_of_iridoid_and_secoiridoid_glycosides_and_comparative_study_on_Radix_gentianae_and_related_adulterants_by_HPLC_analysis_ DB - PRIME DP - Unbound Medicine ER -