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Quantitation of zopiclone and desmethylzopiclone in human plasma by high-performance liquid chromatography using fluorescence detection.
Biomed Chromatogr. 2006 Aug; 20(8):794-9.BC

Abstract

A simple, reliable HPLC method using fluorescence detection (excitation 307 and emission 483 nm) was developed and validated for simultaneous quantitation of zopiclone and its metabolite desmethylzopiclone in human plasma. Following a single-step liquid-liquid extraction, the analytes and internal standard (zaleplon) were separated using an isocratic mobile phase on a reversed-phase C18 column. The lower limit of quantitation was 3 ng/mL for zopiclone and 6 ng/mL for desmethylzopiclone with a relative standard deviation of less than 5%. A linear dynamic range of 3-300 ng/mL for zopiclone and of 6-500 ng/mL for desmethylzopiclone was established. This HPLC method was validated with between-batch precision of 1.7-4.2% and 3.2-7.5% for zopiclone and desmethylzopiclone respectively. The between-batch accuracy was 99.4-111.5% and 101.6-104.8% for zopiclone and desmethylzopiclone, respectively. Frequently coadministered drugs did not interfere with the described methodology. Stability of zopiclone and desmethylzopiclone in plasma was excellent, with no evidence of degradation during sample processing (autosampler) and 30 days' storage in a freezer. This validated method is simple and repeatable enough to be used in pharmacokinetic studies.

Authors+Show Affiliations

Nirogi RV
Biopharmaceutical Research, Suven Life Sciences Ltd, Serene Chambers, Road 7, Banjara Hills, Hyderabad 500034, India. ramakrishna_nirogi@yahoo.co.in
Kandikere VN
No affiliation info available
Mudigonda K
No affiliation info available

MeSH

Azabicyclo CompoundsChromatography, High Pressure LiquidDrug StabilityFluorescenceHumansPiperazinesReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

16292747

Citation

Nirogi, Ramakrishna V S., et al. "Quantitation of Zopiclone and Desmethylzopiclone in Human Plasma By High-performance Liquid Chromatography Using Fluorescence Detection." Biomedical Chromatography : BMC, vol. 20, no. 8, 2006, pp. 794-9.
Nirogi RV, Kandikere VN, Mudigonda K. Quantitation of zopiclone and desmethylzopiclone in human plasma by high-performance liquid chromatography using fluorescence detection. Biomed Chromatogr. 2006;20(8):794-9.
Nirogi, R. V., Kandikere, V. N., & Mudigonda, K. (2006). Quantitation of zopiclone and desmethylzopiclone in human plasma by high-performance liquid chromatography using fluorescence detection. Biomedical Chromatography : BMC, 20(8), 794-9.
Nirogi RV, Kandikere VN, Mudigonda K. Quantitation of Zopiclone and Desmethylzopiclone in Human Plasma By High-performance Liquid Chromatography Using Fluorescence Detection. Biomed Chromatogr. 2006;20(8):794-9. PubMed PMID: 16292747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of zopiclone and desmethylzopiclone in human plasma by high-performance liquid chromatography using fluorescence detection. AU - Nirogi,Ramakrishna V S, AU - Kandikere,Vishwottam N, AU - Mudigonda,Koteshwara, PY - 2005/11/18/pubmed PY - 2006/10/3/medline PY - 2005/11/18/entrez SP - 794 EP - 9 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 20 IS - 8 N2 - A simple, reliable HPLC method using fluorescence detection (excitation 307 and emission 483 nm) was developed and validated for simultaneous quantitation of zopiclone and its metabolite desmethylzopiclone in human plasma. Following a single-step liquid-liquid extraction, the analytes and internal standard (zaleplon) were separated using an isocratic mobile phase on a reversed-phase C18 column. The lower limit of quantitation was 3 ng/mL for zopiclone and 6 ng/mL for desmethylzopiclone with a relative standard deviation of less than 5%. A linear dynamic range of 3-300 ng/mL for zopiclone and of 6-500 ng/mL for desmethylzopiclone was established. This HPLC method was validated with between-batch precision of 1.7-4.2% and 3.2-7.5% for zopiclone and desmethylzopiclone respectively. The between-batch accuracy was 99.4-111.5% and 101.6-104.8% for zopiclone and desmethylzopiclone, respectively. Frequently coadministered drugs did not interfere with the described methodology. Stability of zopiclone and desmethylzopiclone in plasma was excellent, with no evidence of degradation during sample processing (autosampler) and 30 days' storage in a freezer. This validated method is simple and repeatable enough to be used in pharmacokinetic studies. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/16292747/Quantitation_of_zopiclone_and_desmethylzopiclone_in_human_plasma_by_high_performance_liquid_chromatography_using_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -