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[Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Aug 30; 23(4):209-12.ZJ

Abstract

OBJECTIVE

To develop and identify monoclonal antibodies (McAbs) against adult worm of Angiostrongylus cantonensis and observe its applicability.

METHODS

BALB/c mice were immunized with soluble antigen of adult worms of A. cantonensis. The spleen cells of immunized mice were fused with myeloma cell, and the hybridoma secreting high titer of McAbs with high specificity was screened. By using the McAbs, serum of angiostrongyliasis patient and sera of the rats infected with A. cantonensis were detected by Western blotting and double antibody sandwich ELISA respectively.

RESULTS

Three McAbs were established (2A2, 3F1, 4H2), which all showed no cross reaction with antigens of Schistosoma japonicum, Paragonimus westermani, Cysticercus cellulosae and Trichinella spiralis. Western blotting analysis demonstrated that the three McAbs recognized a Mr 15,000 soluble antigen of adult worm of A. cantonensis and recognized the Mr 24,000 and Mr 15,000 circulating antigens from the serum of angiostrongyliasis patient. The double antibody sandwich ELISA detection showed a positive rate of 76.5%.

CONCLUSION

Three hybridoma cell lines against adult worm of A. cantonensis have been established which secret high titer of McAbs with high specificity and seem promising in detecting the circulating antigen of the angiostrongyliasis patient.

Authors+Show Affiliations

Tan F
Department of Parasitology, Wenzhou Medical College, Wenzhou 325035, China.
Pan CW
No affiliation info available
Liiang SH
No affiliation info available
Huang HC
No affiliation info available

MeSH

Angiostrongylus cantonensisAnimalsAntibodies, HelminthAntibodies, MonoclonalAntibody SpecificityAntigens, HelminthBlotting, WesternEnzyme-Linked Immunosorbent AssayFemaleHumansMiceMice, Inbred BALB CSnailsStrongylida Infections

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16296607

Citation

Tan, Feng, et al. "[Preparation and Preliminary Application of Monoclonal Antibodies Against Adult Worm of Angiostrongylus Cantonensis]." Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, vol. 23, no. 4, 2005, pp. 209-12.
Tan F, Pan CW, Liiang SH, et al. [Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005;23(4):209-12.
Tan, F., Pan, C. W., Liiang, S. H., & Huang, H. C. (2005). [Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, 23(4), 209-12.
Tan F, et al. [Preparation and Preliminary Application of Monoclonal Antibodies Against Adult Worm of Angiostrongylus Cantonensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Aug 30;23(4):209-12. PubMed PMID: 16296607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis]. AU - Tan,Feng, AU - Pan,Chang-wang, AU - Liiang,Shao-hui, AU - Huang,Hui-cong, PY - 2005/11/22/pubmed PY - 2009/2/10/medline PY - 2005/11/22/entrez SP - 209 EP - 12 JF - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases JO - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi VL - 23 IS - 4 N2 - OBJECTIVE: To develop and identify monoclonal antibodies (McAbs) against adult worm of Angiostrongylus cantonensis and observe its applicability. METHODS: BALB/c mice were immunized with soluble antigen of adult worms of A. cantonensis. The spleen cells of immunized mice were fused with myeloma cell, and the hybridoma secreting high titer of McAbs with high specificity was screened. By using the McAbs, serum of angiostrongyliasis patient and sera of the rats infected with A. cantonensis were detected by Western blotting and double antibody sandwich ELISA respectively. RESULTS: Three McAbs were established (2A2, 3F1, 4H2), which all showed no cross reaction with antigens of Schistosoma japonicum, Paragonimus westermani, Cysticercus cellulosae and Trichinella spiralis. Western blotting analysis demonstrated that the three McAbs recognized a Mr 15,000 soluble antigen of adult worm of A. cantonensis and recognized the Mr 24,000 and Mr 15,000 circulating antigens from the serum of angiostrongyliasis patient. The double antibody sandwich ELISA detection showed a positive rate of 76.5%. CONCLUSION: Three hybridoma cell lines against adult worm of A. cantonensis have been established which secret high titer of McAbs with high specificity and seem promising in detecting the circulating antigen of the angiostrongyliasis patient. SN - 1000-7423 UR - https://www.unboundmedicine.com/medline/citation/16296607/[Preparation_and_preliminary_application_of_monoclonal_antibodies_against_adult_worm_of_Angiostrongylus_cantonensis]_ DB - PRIME DP - Unbound Medicine ER -