Tags

Type your tag names separated by a space and hit enter

Rapid and ultrasensitive determination of ephedrine and pseudoephedrine derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein by micellar electrokinetic chromatography with laser-induced fluorescence detection.
J Chromatogr A. 2006 Jan 13; 1102(1-2):273-9.JC

Abstract

This paper presents a micellar electrokinetic chromatography method with laser-induced fluorescence detection to analyze ephedrine (E) and pseudoephedrine (PE) after derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein. The optimum derivatization conditions were: 0.05 M Na2CO(3/NaHCO3 (pH 9.5), reaction time 30 min at 45 degrees C, molar ratio of DTAF to E and PE mixture 20:1. The baseline separation was achieved within 8 min with running buffer composed of 20 mM borate+20 mM SDS+15% acetonitrile (v/v) (adjusted pH 9.8), and applied voltage of 20 kV. Good linearity relationships (correlation coefficients: 0.9906 for E and 0.9941 for PE) between the peak heights and concentration of the analytes were obtained (2.5-50 ngmL(-1)). The detection limits for E and PE were 3.85 x 10(-4) and 1.41 x 10(-4)ngmL(-1), respectively, which indicated that the proposed method surpassed other chromatographic alternatives in terms of limit of detection by at least 10(3) folds. The method was applied to the analysis of the two alkaloids in ephedra herb plants and its preparations with recoveries in the range of 89.6-107.0%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16297394

Citation

Wang, Weiping, et al. "Rapid and Ultrasensitive Determination of Ephedrine and Pseudoephedrine Derivatizated With 5-(4,6-dichloro-s-triazin-2-ylamino) Fluorescein By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection." Journal of Chromatography. A, vol. 1102, no. 1-2, 2006, pp. 273-9.
Wang W, Li C, Li Y, et al. Rapid and ultrasensitive determination of ephedrine and pseudoephedrine derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein by micellar electrokinetic chromatography with laser-induced fluorescence detection. J Chromatogr A. 2006;1102(1-2):273-9.
Wang, W., Li, C., Li, Y., Hu, Z., & Chen, X. (2006). Rapid and ultrasensitive determination of ephedrine and pseudoephedrine derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein by micellar electrokinetic chromatography with laser-induced fluorescence detection. Journal of Chromatography. A, 1102(1-2), 273-9.
Wang W, et al. Rapid and Ultrasensitive Determination of Ephedrine and Pseudoephedrine Derivatizated With 5-(4,6-dichloro-s-triazin-2-ylamino) Fluorescein By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection. J Chromatogr A. 2006 Jan 13;1102(1-2):273-9. PubMed PMID: 16297394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and ultrasensitive determination of ephedrine and pseudoephedrine derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein by micellar electrokinetic chromatography with laser-induced fluorescence detection. AU - Wang,Weiping, AU - Li,Cunhong, AU - Li,Ying, AU - Hu,Zhide, AU - Chen,Xingguo, Y1 - 2005/11/16/ PY - 2005/06/30/received PY - 2005/09/20/revised PY - 2005/10/11/accepted PY - 2005/11/22/pubmed PY - 2006/3/10/medline PY - 2005/11/22/entrez SP - 273 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1102 IS - 1-2 N2 - This paper presents a micellar electrokinetic chromatography method with laser-induced fluorescence detection to analyze ephedrine (E) and pseudoephedrine (PE) after derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein. The optimum derivatization conditions were: 0.05 M Na2CO(3/NaHCO3 (pH 9.5), reaction time 30 min at 45 degrees C, molar ratio of DTAF to E and PE mixture 20:1. The baseline separation was achieved within 8 min with running buffer composed of 20 mM borate+20 mM SDS+15% acetonitrile (v/v) (adjusted pH 9.8), and applied voltage of 20 kV. Good linearity relationships (correlation coefficients: 0.9906 for E and 0.9941 for PE) between the peak heights and concentration of the analytes were obtained (2.5-50 ngmL(-1)). The detection limits for E and PE were 3.85 x 10(-4) and 1.41 x 10(-4)ngmL(-1), respectively, which indicated that the proposed method surpassed other chromatographic alternatives in terms of limit of detection by at least 10(3) folds. The method was applied to the analysis of the two alkaloids in ephedra herb plants and its preparations with recoveries in the range of 89.6-107.0%. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16297394/Rapid_and_ultrasensitive_determination_of_ephedrine_and_pseudoephedrine_derivatizated_with_5__46_dichloro_s_triazin_2_ylamino__fluorescein_by_micellar_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(05)01941-2 DB - PRIME DP - Unbound Medicine ER -