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Genome-wide transcription program and expression of the Rta responsive gene of Epstein-Barr virus.
Virology. 2006 Feb 20; 345(2):358-72.V

Abstract

Infection with Epstein-Barr virus (EBV) usually leads to a latent state in B lymphocytes. The virus can be reactivated through two viral transactivators, Zta and Rta, leading to a cascade of gene expression. An EBV DNA array was generated to analyze the pattern of transcription of the entire EBV genome under various conditions. Firstly, a complete set of temporal expression clusters of EBV genes was displayed by analyzing the array data of anti-IgG-induced Akata cells. In addition to assigning genes of unknown function to the various clusters, increasing expression of latent genes, including EBNA2, EBNA3A and EBNA 3C, was observed during virus replication. Secondly, gene expression independent of viral DNA replication was analyzed in PAA blocked Akata cells and in chemically induced Raji cells. Several genes with presumed late functions were found to be expressed with early kinetics and independent of viral DNA replication, suggesting possible novel functions for these genes. Finally, the EBV array was used to identify Rta responsive gene expression in Raji cells, and in the EBV-positive epithelial cells NA, using a Zta siRNA strategy. The array data were confirmed by Northern blotting, RT-PCR and reporter assays. All the information here thus provides a better understanding of the control of EBV lytic gene expression.

Authors+Show Affiliations

Graduate Institute and Department of Microbiology, College of Medicine, National Taiwan University, No. 1, Jen-Ai Rd, 1st section, Taipei 100, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16298410

Citation

Lu, Chih-Chung, et al. "Genome-wide Transcription Program and Expression of the Rta Responsive Gene of Epstein-Barr Virus." Virology, vol. 345, no. 2, 2006, pp. 358-72.
Lu CC, Jeng YY, Tsai CH, et al. Genome-wide transcription program and expression of the Rta responsive gene of Epstein-Barr virus. Virology. 2006;345(2):358-72.
Lu, C. C., Jeng, Y. Y., Tsai, C. H., Liu, M. Y., Yeh, S. W., Hsu, T. Y., & Chen, M. R. (2006). Genome-wide transcription program and expression of the Rta responsive gene of Epstein-Barr virus. Virology, 345(2), 358-72.
Lu CC, et al. Genome-wide Transcription Program and Expression of the Rta Responsive Gene of Epstein-Barr Virus. Virology. 2006 Feb 20;345(2):358-72. PubMed PMID: 16298410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide transcription program and expression of the Rta responsive gene of Epstein-Barr virus. AU - Lu,Chih-Chung, AU - Jeng,Yi-Ying, AU - Tsai,Ching-Hwa, AU - Liu,Mei-Ying, AU - Yeh,Sheng-Wen, AU - Hsu,Tsuey-Ying, AU - Chen,Mei-Ru, Y1 - 2005/11/18/ PY - 2005/07/25/received PY - 2005/09/07/revised PY - 2005/09/28/accepted PY - 2005/11/22/pubmed PY - 2006/3/24/medline PY - 2005/11/22/entrez SP - 358 EP - 72 JF - Virology JO - Virology VL - 345 IS - 2 N2 - Infection with Epstein-Barr virus (EBV) usually leads to a latent state in B lymphocytes. The virus can be reactivated through two viral transactivators, Zta and Rta, leading to a cascade of gene expression. An EBV DNA array was generated to analyze the pattern of transcription of the entire EBV genome under various conditions. Firstly, a complete set of temporal expression clusters of EBV genes was displayed by analyzing the array data of anti-IgG-induced Akata cells. In addition to assigning genes of unknown function to the various clusters, increasing expression of latent genes, including EBNA2, EBNA3A and EBNA 3C, was observed during virus replication. Secondly, gene expression independent of viral DNA replication was analyzed in PAA blocked Akata cells and in chemically induced Raji cells. Several genes with presumed late functions were found to be expressed with early kinetics and independent of viral DNA replication, suggesting possible novel functions for these genes. Finally, the EBV array was used to identify Rta responsive gene expression in Raji cells, and in the EBV-positive epithelial cells NA, using a Zta siRNA strategy. The array data were confirmed by Northern blotting, RT-PCR and reporter assays. All the information here thus provides a better understanding of the control of EBV lytic gene expression. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/16298410/Genome_wide_transcription_program_and_expression_of_the_Rta_responsive_gene_of_Epstein_Barr_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(05)00637-9 DB - PRIME DP - Unbound Medicine ER -