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Evidence of T-helper cell 2 cytokine regulation of chronic otitis media with effusion.
Acta Otolaryngol. 2005 Oct; 125(10):1043-50.AO

Abstract

Cytokine and cellular patterns of effusions may reflect stages of middle ear inflammation. The local interplay between IL-2 and -4 is likely to play a crucial role in the switching of inflammation in the chronic stage. The T-helper cell 2 (Th2) cytokines IL-4, -5 and -13 and the Th2/Th1 cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) regulate the cellular and molecular processes of chronic inflammation in the middle ear and therefore the chronic condition of otitis media with effusion (OME). Early identification of the cytokine and cellular patterns of effusions can be helpful in directing the clinical treatment of OME.We hypothesized that IL-2 and the group of Th2 cytokines regulate chronic inflammation in the middle ear and chronic OME. Effusions from children with persistent OME were analysed to determine the presence of cytokines (the Th1 cytokine IL-2, the Th2 cytokines IL-4, -5 and -13 and the Th1/Th2 cytokine GM-CSF), inflammatory cells (CD4+ T cells, eosinophils, macrophages and neutrophils) and mucin. Cytokines were evaluated by means of a quantitative "sandwich"-type ELISA, inflammatory cells by means of alkaline phosphatase-anti-alkaline phosphatase immunocytostaining and mucin by means of a modified periodic acid-Schiff method based on a slot-blot technique. The cytokine pattern in effusions varied from patient to patient. GM-CSF correlated positively and IL-4 inversely with IL-2 and the increased level of IL-4 may have had an inhibitory effect on IL-2. IL-5 and -13 correlated with IL-4. Inflammatory cells correlated with cytokines as follows: CD4+ T cells with IL-2 and -4; macrophages and neutrophils with GM-CSF; and eosinophils with IL-5. Some cytokine-cellular correlations in effusions were reflected at the clinical level. The mucin content of effusions correlated with the concentrations of IL-4 (>10 pg/ml) and -13, suggesting involvement of IL-4 and -13 in upregulation of the middle ear mucin metabolism.

Authors+Show Affiliations

Institute for Cell and Molecular Biosciences & School of Biomedical Sciences, Medical Faculty, University of Newcastle upon Tyne, Newcastle upon Tyne, UK. Marina.Smirnova@ncl.ac.ukNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16298784

Citation

Smirnova, Marina G., et al. "Evidence of T-helper Cell 2 Cytokine Regulation of Chronic Otitis Media With Effusion." Acta Oto-laryngologica, vol. 125, no. 10, 2005, pp. 1043-50.
Smirnova MG, Birchall JP, Pearson JP. Evidence of T-helper cell 2 cytokine regulation of chronic otitis media with effusion. Acta Otolaryngol. 2005;125(10):1043-50.
Smirnova, M. G., Birchall, J. P., & Pearson, J. P. (2005). Evidence of T-helper cell 2 cytokine regulation of chronic otitis media with effusion. Acta Oto-laryngologica, 125(10), 1043-50.
Smirnova MG, Birchall JP, Pearson JP. Evidence of T-helper Cell 2 Cytokine Regulation of Chronic Otitis Media With Effusion. Acta Otolaryngol. 2005;125(10):1043-50. PubMed PMID: 16298784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of T-helper cell 2 cytokine regulation of chronic otitis media with effusion. AU - Smirnova,Marina G, AU - Birchall,John P, AU - Pearson,Jeffrey P, PY - 2005/11/22/pubmed PY - 2006/1/4/medline PY - 2005/11/22/entrez SP - 1043 EP - 50 JF - Acta oto-laryngologica JO - Acta Otolaryngol VL - 125 IS - 10 N2 - Cytokine and cellular patterns of effusions may reflect stages of middle ear inflammation. The local interplay between IL-2 and -4 is likely to play a crucial role in the switching of inflammation in the chronic stage. The T-helper cell 2 (Th2) cytokines IL-4, -5 and -13 and the Th2/Th1 cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) regulate the cellular and molecular processes of chronic inflammation in the middle ear and therefore the chronic condition of otitis media with effusion (OME). Early identification of the cytokine and cellular patterns of effusions can be helpful in directing the clinical treatment of OME.We hypothesized that IL-2 and the group of Th2 cytokines regulate chronic inflammation in the middle ear and chronic OME. Effusions from children with persistent OME were analysed to determine the presence of cytokines (the Th1 cytokine IL-2, the Th2 cytokines IL-4, -5 and -13 and the Th1/Th2 cytokine GM-CSF), inflammatory cells (CD4+ T cells, eosinophils, macrophages and neutrophils) and mucin. Cytokines were evaluated by means of a quantitative "sandwich"-type ELISA, inflammatory cells by means of alkaline phosphatase-anti-alkaline phosphatase immunocytostaining and mucin by means of a modified periodic acid-Schiff method based on a slot-blot technique. The cytokine pattern in effusions varied from patient to patient. GM-CSF correlated positively and IL-4 inversely with IL-2 and the increased level of IL-4 may have had an inhibitory effect on IL-2. IL-5 and -13 correlated with IL-4. Inflammatory cells correlated with cytokines as follows: CD4+ T cells with IL-2 and -4; macrophages and neutrophils with GM-CSF; and eosinophils with IL-5. Some cytokine-cellular correlations in effusions were reflected at the clinical level. The mucin content of effusions correlated with the concentrations of IL-4 (>10 pg/ml) and -13, suggesting involvement of IL-4 and -13 in upregulation of the middle ear mucin metabolism. SN - 0001-6489 UR - https://www.unboundmedicine.com/medline/citation/16298784/Evidence_of_T_helper_cell_2_cytokine_regulation_of_chronic_otitis_media_with_effusion_ L2 - https://www.tandfonline.com/doi/full/10.1080/00016480510035449 DB - PRIME DP - Unbound Medicine ER -