Tags

Type your tag names separated by a space and hit enter

Suppression of lipopolysaccharide-induced cytokine production of gingival fibroblasts by a soybean, Kunitz trypsin inhibitor.
J Periodontal Res. 2005 Dec; 40(6):461-8.JP

Abstract

BACKGROUND

Human bikunin, a Kunitz-type trypsin inhibitor, inhibits inflammation by down-regulating the expression of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) in tumor cells and inflammatory cells.

OBJECTIVES

We analyzed the effect of a soybean-derived Kunitz trypsin inhibitor (KTI) on TNF-alpha production in human gingival fibroblasts stimulated by lipopolysaccharide (LPS), an inflammatory inducer.

MATERIAL AND METHODS

Mitogen-activated protein kinase (MAPK) activation and cytokine levels were monitored using western blot and a specific enzyme-linked immunosorbent assay (ELISA).

RESULTS

Here, we show (i) a soybean KTI abrogates LPS-induced up-regulation of TNF-alpha mRNA and protein expression in a dose-dependent manner in gingival fibroblasts, (ii) KTI also blocks the induction of TNF-alpha target molecules interleukin-1beta (IL-1beta) and IL-6 proteins, (iii) inhibition by KTI of TNF-alpha induction correlates with the suppressive capacity of extracellular signal-regulated kinase 1/2 (ERK1/2) and p38 signaling pathways, implicating repressed ERK1/2 and p38 signalings in the inhibition, and (iv) pretreatment of cells with KTI blocked LPS-induced nuclear factor kappaB (NFkappaB) activation.

CONCLUSION

Our results indicate that KTI inhibits LPS-induced up-regulation of cytokine expression possibly through suppression of ERK1/2 and p38 kinase-mediated NFkappaB activation. These findings may identify anti-inflammatory properties of KTI at the level of gingival fibroblasts and may be relevant to the use of KTI in modulating inflammation, including periodontal disease.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan. hirokoba@hama-med.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16302924

Citation

Kobayashi, Hiroshi, et al. "Suppression of Lipopolysaccharide-induced Cytokine Production of Gingival Fibroblasts By a Soybean, Kunitz Trypsin Inhibitor." Journal of Periodontal Research, vol. 40, no. 6, 2005, pp. 461-8.
Kobayashi H, Yoshida R, Kanada Y, et al. Suppression of lipopolysaccharide-induced cytokine production of gingival fibroblasts by a soybean, Kunitz trypsin inhibitor. J Periodontal Res. 2005;40(6):461-8.
Kobayashi, H., Yoshida, R., Kanada, Y., Fukuda, Y., Yagyu, T., Inagaki, K., Kondo, T., Kurita, N., Suzuki, M., Kanayama, N., & Terao, T. (2005). Suppression of lipopolysaccharide-induced cytokine production of gingival fibroblasts by a soybean, Kunitz trypsin inhibitor. Journal of Periodontal Research, 40(6), 461-8.
Kobayashi H, et al. Suppression of Lipopolysaccharide-induced Cytokine Production of Gingival Fibroblasts By a Soybean, Kunitz Trypsin Inhibitor. J Periodontal Res. 2005;40(6):461-8. PubMed PMID: 16302924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppression of lipopolysaccharide-induced cytokine production of gingival fibroblasts by a soybean, Kunitz trypsin inhibitor. AU - Kobayashi,Hiroshi, AU - Yoshida,Ryuji, AU - Kanada,Yasufumi, AU - Fukuda,Yoichi, AU - Yagyu,Tatsuo, AU - Inagaki,Kiyokazu, AU - Kondo,Toshiharu, AU - Kurita,Noriyuki, AU - Suzuki,Mika, AU - Kanayama,Naohiro, AU - Terao,Toshihiko, PY - 2005/11/24/pubmed PY - 2006/1/13/medline PY - 2005/11/24/entrez SP - 461 EP - 8 JF - Journal of periodontal research JO - J Periodontal Res VL - 40 IS - 6 N2 - BACKGROUND: Human bikunin, a Kunitz-type trypsin inhibitor, inhibits inflammation by down-regulating the expression of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) in tumor cells and inflammatory cells. OBJECTIVES: We analyzed the effect of a soybean-derived Kunitz trypsin inhibitor (KTI) on TNF-alpha production in human gingival fibroblasts stimulated by lipopolysaccharide (LPS), an inflammatory inducer. MATERIAL AND METHODS: Mitogen-activated protein kinase (MAPK) activation and cytokine levels were monitored using western blot and a specific enzyme-linked immunosorbent assay (ELISA). RESULTS: Here, we show (i) a soybean KTI abrogates LPS-induced up-regulation of TNF-alpha mRNA and protein expression in a dose-dependent manner in gingival fibroblasts, (ii) KTI also blocks the induction of TNF-alpha target molecules interleukin-1beta (IL-1beta) and IL-6 proteins, (iii) inhibition by KTI of TNF-alpha induction correlates with the suppressive capacity of extracellular signal-regulated kinase 1/2 (ERK1/2) and p38 signaling pathways, implicating repressed ERK1/2 and p38 signalings in the inhibition, and (iv) pretreatment of cells with KTI blocked LPS-induced nuclear factor kappaB (NFkappaB) activation. CONCLUSION: Our results indicate that KTI inhibits LPS-induced up-regulation of cytokine expression possibly through suppression of ERK1/2 and p38 kinase-mediated NFkappaB activation. These findings may identify anti-inflammatory properties of KTI at the level of gingival fibroblasts and may be relevant to the use of KTI in modulating inflammation, including periodontal disease. SN - 0022-3484 UR - https://www.unboundmedicine.com/medline/citation/16302924/Suppression_of_lipopolysaccharide_induced_cytokine_production_of_gingival_fibroblasts_by_a_soybean_Kunitz_trypsin_inhibitor_ L2 - https://doi.org/10.1111/j.1600-0765.2005.00824.x DB - PRIME DP - Unbound Medicine ER -