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Tissue-specific expression and drought responsiveness of cell-wall invertase genes of rice at flowering.
Plant Mol Biol. 2005 Dec; 59(6):945-64.PM

Abstract

Drought stress near heading reduces grain yield in rice cultivars by inhibiting processes such as anther dehiscence and panicle exsertion. Because cell-wall invertases play an important role in carbon allocation to developing organs, we examined the tissue-specific expression and drought sensitivity of the corresponding genes (OsCIN1-9) at heading in the widely grown cultivar IR64. OsCIN1-5,8 were expressed to varying degrees in flag leaf, panicle, anthers and peduncle at 1 day before heading (1 DBH). When water was withheld for 2 days starting 3 DBH, anthesis and peduncle elongation were halted. At the same time, transcript levels for OsCIN1-5,8 genes were all markedly down-regulated in anthers and/or peduncles but were not affected in flag leaves. Re-watering allowed anthesis and peduncle elongation to proceed and restored expression of OsCIN1-5,8. We conclude that cell-wall invertase genes, as a class, respond rapidly to water deficit in anthers and peduncles and through a reduction in sink strength help to coordinate a delay in anthesis and heading. By contrast, vacuolar invertase OsVIN2 was up-regulated by drought stress in flag leaves, panicles, anthers and peduncles. Although OsCIN1-3,5,8 were active in the peduncle, only OsCIN2 was expressed strongly and preferentially at the base, where cell division and cell elongation occur. OsCIN2 was expressed principally in the primary and secondary vascular systems, consistent with a role in diverting sucrose from the phloem to the dividing and expanding cells of the peduncle, whereas the less abundant OsCIN1,3,5,8 transcripts were found principally in parenchyma cells. The OsCIN2 transcript levels in the base were highest at 1 DBH, when rapid peduncle elongation began. Drought stress halted peduncle elongation and reduced OsCIN2 transcript level to 8% of the control level. On re-watering, peduncle elongation was restored and OsCIN2 transcript level recovered to 24% of the control. The abscisic acid (ABA) level of peduncles increased 7-fold on drought stress and returned to the control level on re-watering. Detached peduncles floated on water elongated little and lost all OsCIN2 transcripts, but on 50-100 microM GA3 they elongated rapidly and maintained high OsCIN2 transcript levels. ABA antagonized both peduncle elongation and maintenance of OsCIN2 transcript levels. We conclude that this antagonism is a potential intervention point for breeding strategies directed at enhancing panicle exsertion during or after drought stress at heading.

Authors+Show Affiliations

Ji XM
Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.
Raveendran M
No affiliation info available
Oane R
No affiliation info available
Ismail A
No affiliation info available
Lafitte R
No affiliation info available
Bruskiewich R
No affiliation info available
Cheng SH
No affiliation info available
Bennett J
No affiliation info available

MeSH

Abscisic AcidCell WallDNA PrimersDown-RegulationGene Expression Regulation, EnzymologicGene Expression Regulation, PlantGibberellinsIn Situ HybridizationOligonucleotides, AntisenseOryzaRNARNA, MessengerReverse Transcriptase Polymerase Chain ReactionStarchTime FactorsTissue DistributionVacuolesbeta-Fructofuranosidase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16307368

Citation

Ji, X M., et al. "Tissue-specific Expression and Drought Responsiveness of Cell-wall Invertase Genes of Rice at Flowering." Plant Molecular Biology, vol. 59, no. 6, 2005, pp. 945-64.
Ji XM, Raveendran M, Oane R, et al. Tissue-specific expression and drought responsiveness of cell-wall invertase genes of rice at flowering. Plant Mol Biol. 2005;59(6):945-64.
Ji, X. M., Raveendran, M., Oane, R., Ismail, A., Lafitte, R., Bruskiewich, R., Cheng, S. H., & Bennett, J. (2005). Tissue-specific expression and drought responsiveness of cell-wall invertase genes of rice at flowering. Plant Molecular Biology, 59(6), 945-64.
Ji XM, et al. Tissue-specific Expression and Drought Responsiveness of Cell-wall Invertase Genes of Rice at Flowering. Plant Mol Biol. 2005;59(6):945-64. PubMed PMID: 16307368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue-specific expression and drought responsiveness of cell-wall invertase genes of rice at flowering. AU - Ji,X M, AU - Raveendran,M, AU - Oane,R, AU - Ismail,A, AU - Lafitte,R, AU - Bruskiewich,R, AU - Cheng,S H, AU - Bennett,J, PY - 2004/12/16/received PY - 2005/08/23/accepted PY - 2005/11/25/pubmed PY - 2006/2/7/medline PY - 2005/11/25/entrez SP - 945 EP - 64 JF - Plant molecular biology JO - Plant Mol Biol VL - 59 IS - 6 N2 - Drought stress near heading reduces grain yield in rice cultivars by inhibiting processes such as anther dehiscence and panicle exsertion. Because cell-wall invertases play an important role in carbon allocation to developing organs, we examined the tissue-specific expression and drought sensitivity of the corresponding genes (OsCIN1-9) at heading in the widely grown cultivar IR64. OsCIN1-5,8 were expressed to varying degrees in flag leaf, panicle, anthers and peduncle at 1 day before heading (1 DBH). When water was withheld for 2 days starting 3 DBH, anthesis and peduncle elongation were halted. At the same time, transcript levels for OsCIN1-5,8 genes were all markedly down-regulated in anthers and/or peduncles but were not affected in flag leaves. Re-watering allowed anthesis and peduncle elongation to proceed and restored expression of OsCIN1-5,8. We conclude that cell-wall invertase genes, as a class, respond rapidly to water deficit in anthers and peduncles and through a reduction in sink strength help to coordinate a delay in anthesis and heading. By contrast, vacuolar invertase OsVIN2 was up-regulated by drought stress in flag leaves, panicles, anthers and peduncles. Although OsCIN1-3,5,8 were active in the peduncle, only OsCIN2 was expressed strongly and preferentially at the base, where cell division and cell elongation occur. OsCIN2 was expressed principally in the primary and secondary vascular systems, consistent with a role in diverting sucrose from the phloem to the dividing and expanding cells of the peduncle, whereas the less abundant OsCIN1,3,5,8 transcripts were found principally in parenchyma cells. The OsCIN2 transcript levels in the base were highest at 1 DBH, when rapid peduncle elongation began. Drought stress halted peduncle elongation and reduced OsCIN2 transcript level to 8% of the control level. On re-watering, peduncle elongation was restored and OsCIN2 transcript level recovered to 24% of the control. The abscisic acid (ABA) level of peduncles increased 7-fold on drought stress and returned to the control level on re-watering. Detached peduncles floated on water elongated little and lost all OsCIN2 transcripts, but on 50-100 microM GA3 they elongated rapidly and maintained high OsCIN2 transcript levels. ABA antagonized both peduncle elongation and maintenance of OsCIN2 transcript levels. We conclude that this antagonism is a potential intervention point for breeding strategies directed at enhancing panicle exsertion during or after drought stress at heading. SN - 0167-4412 UR - https://www.unboundmedicine.com/medline/citation/16307368/Tissue_specific_expression_and_drought_responsiveness_of_cell_wall_invertase_genes_of_rice_at_flowering_ DB - PRIME DP - Unbound Medicine ER -