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Inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells by sulfonylurea glibenclamide.
Eur J Pharmacol. 2005 Dec 19; 527(1-3):23-30.EJ

Abstract

The present study investigates the effect of sulfonylurea glibenclamide on the cytotoxicity of 1-methyl-4-phenylpyridinium (MPP+) in differentiated PC12 cells in relation to changes in the mitochondrial membrane permeability. Glibenclamide and tolbutamide reduced the MPP+-induced cell death and GSH depletion concentration dependently with a maximal inhibitory effect at 5-10 microM. Despite the toxic effect at 20 microM, sulfonylureas showed an inhibitory effect. N-Acetylcysteine, superoxide dismutase, catalase, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and Mn(III) tetrakis(4-benzoic acid)porphyrin chloride inhibited the cytotoxicity of MPP+. Glibenclamide attenuated the nuclear damage, changes in the mitochondrial membrane permeability, caspase-3 activation and formation of reactive oxygen species due to MPP+ in PC12 cells. The results show that glibenclamide may reduce the MPP+-induced viability loss in PC12 cells by suppressing the changes in the mitochondrial membrane permeability, leading to the release of cytochrome c and subsequent activation of caspase-3, which are associated with the increased reactive oxygen species formation and depletion of GSH.

Authors+Show Affiliations

Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea. leecs@cau.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16310184

Citation

Lee, Chung Soo, et al. "Inhibition of 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in PC12 Cells By Sulfonylurea Glibenclamide." European Journal of Pharmacology, vol. 527, no. 1-3, 2005, pp. 23-30.
Lee CS, Kim YJ, Ko HH, et al. Inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells by sulfonylurea glibenclamide. Eur J Pharmacol. 2005;527(1-3):23-30.
Lee, C. S., Kim, Y. J., Ko, H. H., & Han, E. S. (2005). Inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells by sulfonylurea glibenclamide. European Journal of Pharmacology, 527(1-3), 23-30.
Lee CS, et al. Inhibition of 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in PC12 Cells By Sulfonylurea Glibenclamide. Eur J Pharmacol. 2005 Dec 19;527(1-3):23-30. PubMed PMID: 16310184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells by sulfonylurea glibenclamide. AU - Lee,Chung Soo, AU - Kim,Yun Jeong, AU - Ko,Hyun Hee, AU - Han,Eun Sook, Y1 - 2005/11/23/ PY - 2005/05/26/received PY - 2005/09/27/revised PY - 2005/10/07/accepted PY - 2005/11/29/pubmed PY - 2006/2/9/medline PY - 2005/11/29/entrez SP - 23 EP - 30 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 527 IS - 1-3 N2 - The present study investigates the effect of sulfonylurea glibenclamide on the cytotoxicity of 1-methyl-4-phenylpyridinium (MPP+) in differentiated PC12 cells in relation to changes in the mitochondrial membrane permeability. Glibenclamide and tolbutamide reduced the MPP+-induced cell death and GSH depletion concentration dependently with a maximal inhibitory effect at 5-10 microM. Despite the toxic effect at 20 microM, sulfonylureas showed an inhibitory effect. N-Acetylcysteine, superoxide dismutase, catalase, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and Mn(III) tetrakis(4-benzoic acid)porphyrin chloride inhibited the cytotoxicity of MPP+. Glibenclamide attenuated the nuclear damage, changes in the mitochondrial membrane permeability, caspase-3 activation and formation of reactive oxygen species due to MPP+ in PC12 cells. The results show that glibenclamide may reduce the MPP+-induced viability loss in PC12 cells by suppressing the changes in the mitochondrial membrane permeability, leading to the release of cytochrome c and subsequent activation of caspase-3, which are associated with the increased reactive oxygen species formation and depletion of GSH. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/16310184/Inhibition_of_1_methyl_4_phenylpyridinium_induced_mitochondrial_dysfunction_and_cell_death_in_PC12_cells_by_sulfonylurea_glibenclamide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(05)01066-6 DB - PRIME DP - Unbound Medicine ER -