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HLA-G is expressed by the glandular epithelium of peritoneal endometriosis but not in eutopic endometrium.
Hum Reprod 2006; 21(4):864-9HR

Abstract

BACKGROUND

HLA-G is a major histocompatability antigen with documented immune-regulatory function. Various epithelial cancers and tissue allografts have been noted to express HLA-G, which is postulated to aid in their escape from immunosurveillance. We evaluated peritoneal endometriosis and eutopic endometrium for the expression of HLA-G protein and gene transcript.

METHODS

Two experiments were performed: (i) archived tissue blocks from peritoneal endometriotic lesions (n = 15) and eutopic endometrium (n = 12) were evaluated for extent of protein immunostaining, and (ii) eutopic endometrial biopsies from women without (n = 17) and with (n = 24) endometriosis, and peritoneal endometriotic lesions (n = 14) were evaluated for presence of RNA transcript by in situ hybridization.

RESULTS

HLA-G protein localized in the glandular epithelium of 14 of 15 (93.3%) peritoneal endometriotic lesions, but not in stromal cells. HLA-G protein staining was absent in endometrial biopsies (n = 12). HLA-G gene transcript localized to the glandular epithelium in 13 of 14 (92.8%) peritoneal endometriotic lesions. HLA-G transcript was never observed in eutopic endometrium, regardless of cycle stage or whether from women with (n = 24) or without (n = 18) endometriosis.

CONCLUSIONS

HLA-G is expressed by endometriotic glandular epithelium but not by eutopic endometrium under normal conditions. Differential expression of HLA-G suggests that peritoneal inflammation or cellular stress may up-regulate mechanisms to promote ectopic endometrial survival.

Authors+Show Affiliations

Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive and Perinatal Research, University of Missouri-Columbia, Missouri 65212, USA. barrierb@health.missouri.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16311290

Citation

Barrier, B F., et al. "HLA-G Is Expressed By the Glandular Epithelium of Peritoneal Endometriosis but Not in Eutopic Endometrium." Human Reproduction (Oxford, England), vol. 21, no. 4, 2006, pp. 864-9.
Barrier BF, Kendall BS, Ryan CE, et al. HLA-G is expressed by the glandular epithelium of peritoneal endometriosis but not in eutopic endometrium. Hum Reprod. 2006;21(4):864-9.
Barrier, B. F., Kendall, B. S., Ryan, C. E., & Sharpe-Timms, K. L. (2006). HLA-G is expressed by the glandular epithelium of peritoneal endometriosis but not in eutopic endometrium. Human Reproduction (Oxford, England), 21(4), pp. 864-9.
Barrier BF, et al. HLA-G Is Expressed By the Glandular Epithelium of Peritoneal Endometriosis but Not in Eutopic Endometrium. Hum Reprod. 2006;21(4):864-9. PubMed PMID: 16311290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HLA-G is expressed by the glandular epithelium of peritoneal endometriosis but not in eutopic endometrium. AU - Barrier,B F, AU - Kendall,B S, AU - Ryan,C E, AU - Sharpe-Timms,K L, Y1 - 2005/11/25/ PY - 2005/11/29/pubmed PY - 2006/6/6/medline PY - 2005/11/29/entrez SP - 864 EP - 9 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 21 IS - 4 N2 - BACKGROUND: HLA-G is a major histocompatability antigen with documented immune-regulatory function. Various epithelial cancers and tissue allografts have been noted to express HLA-G, which is postulated to aid in their escape from immunosurveillance. We evaluated peritoneal endometriosis and eutopic endometrium for the expression of HLA-G protein and gene transcript. METHODS: Two experiments were performed: (i) archived tissue blocks from peritoneal endometriotic lesions (n = 15) and eutopic endometrium (n = 12) were evaluated for extent of protein immunostaining, and (ii) eutopic endometrial biopsies from women without (n = 17) and with (n = 24) endometriosis, and peritoneal endometriotic lesions (n = 14) were evaluated for presence of RNA transcript by in situ hybridization. RESULTS: HLA-G protein localized in the glandular epithelium of 14 of 15 (93.3%) peritoneal endometriotic lesions, but not in stromal cells. HLA-G protein staining was absent in endometrial biopsies (n = 12). HLA-G gene transcript localized to the glandular epithelium in 13 of 14 (92.8%) peritoneal endometriotic lesions. HLA-G transcript was never observed in eutopic endometrium, regardless of cycle stage or whether from women with (n = 24) or without (n = 18) endometriosis. CONCLUSIONS: HLA-G is expressed by endometriotic glandular epithelium but not by eutopic endometrium under normal conditions. Differential expression of HLA-G suggests that peritoneal inflammation or cellular stress may up-regulate mechanisms to promote ectopic endometrial survival. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/16311290/HLA_G_is_expressed_by_the_glandular_epithelium_of_peritoneal_endometriosis_but_not_in_eutopic_endometrium_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/dei408 DB - PRIME DP - Unbound Medicine ER -