Tags

Type your tag names separated by a space and hit enter

Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control.
Mol Microbiol. 2005 Dec; 58(5):1421-9.MM

Abstract

The Escherichia coli ompA mRNA, encoding a highly abundant outer membrane protein, has served as a model for regulated mRNA decay in bacteria. The half-life of this transcript correlates inversely with the bacterial growth rate and is growth stage-dependent. The stability of the messenger is determined by the 5'-untranslated region which possesses cleavage sites for RNase E. Hfq binds to this region, is essential for controlling the stability and has been suggested to directly regulate ompA mRNA decay. Here we report that the 78 nucleotide SraD RNA, which is highly conserved among Enterobacteriaceae, acts in destabilizing the ompA transcript when rapidly grown cells enter the stationary phase of growth. During this growth-stage the expression of SraD RNA becomes strongly increased. The SraD-mediated decay of ompA mRNA depends on Hfq and in vitro studies revealed that Hfq facilitates binding of the regulatory RNA to the translational initiation region of the messenger. Deletion of sraD, however, does not significantly affect the stability of the ompA mRNA in slowly growing cells. Our results indicate that distinct regulatory circuits are responsible for growth phase- and growth rate-dependent control of the ompA mRNA stability.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16313626

Citation

Rasmussen, Anders Aamann, et al. "Regulation of ompA mRNA Stability: the Role of a Small Regulatory RNA in Growth Phase-dependent Control." Molecular Microbiology, vol. 58, no. 5, 2005, pp. 1421-9.
Rasmussen AA, Eriksen M, Gilany K, et al. Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control. Mol Microbiol. 2005;58(5):1421-9.
Rasmussen, A. A., Eriksen, M., Gilany, K., Udesen, C., Franch, T., Petersen, C., & Valentin-Hansen, P. (2005). Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control. Molecular Microbiology, 58(5), 1421-9.
Rasmussen AA, et al. Regulation of ompA mRNA Stability: the Role of a Small Regulatory RNA in Growth Phase-dependent Control. Mol Microbiol. 2005;58(5):1421-9. PubMed PMID: 16313626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control. AU - Rasmussen,Anders Aamann, AU - Eriksen,Maiken, AU - Gilany,Kambiz, AU - Udesen,Christina, AU - Franch,Thomas, AU - Petersen,Carsten, AU - Valentin-Hansen,Poul, PY - 2005/11/30/pubmed PY - 2006/1/6/medline PY - 2005/11/30/entrez SP - 1421 EP - 9 JF - Molecular microbiology JO - Mol Microbiol VL - 58 IS - 5 N2 - The Escherichia coli ompA mRNA, encoding a highly abundant outer membrane protein, has served as a model for regulated mRNA decay in bacteria. The half-life of this transcript correlates inversely with the bacterial growth rate and is growth stage-dependent. The stability of the messenger is determined by the 5'-untranslated region which possesses cleavage sites for RNase E. Hfq binds to this region, is essential for controlling the stability and has been suggested to directly regulate ompA mRNA decay. Here we report that the 78 nucleotide SraD RNA, which is highly conserved among Enterobacteriaceae, acts in destabilizing the ompA transcript when rapidly grown cells enter the stationary phase of growth. During this growth-stage the expression of SraD RNA becomes strongly increased. The SraD-mediated decay of ompA mRNA depends on Hfq and in vitro studies revealed that Hfq facilitates binding of the regulatory RNA to the translational initiation region of the messenger. Deletion of sraD, however, does not significantly affect the stability of the ompA mRNA in slowly growing cells. Our results indicate that distinct regulatory circuits are responsible for growth phase- and growth rate-dependent control of the ompA mRNA stability. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/16313626/Regulation_of_ompA_mRNA_stability:_the_role_of_a_small_regulatory_RNA_in_growth_phase_dependent_control_ L2 - https://doi.org/10.1111/j.1365-2958.2005.04911.x DB - PRIME DP - Unbound Medicine ER -