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[Effects of interleukin-18 on asthmatic airway inflammation: experimental study of guinea pig asthmatic model].
Zhonghua Yi Xue Za Zhi. 2005 Jul 27; 85(28):1995-8.ZY

Abstract

OBJECTIVE

To investigate the effects of Interleukin-18 (IL-18) on asthmatic airway inflammation.

METHODS

Thirty healthy adult male guinea pigs were randomly divided into 3 equal groups: asthmatic model group (Group A, undergoing intraperitoneally injection of ovalbumin (OVA) once and spraying of OVA aerosol once a day for 5 days; control group (Group B), undergoing intraperitoneally injection of OVA once and spraying of normal saline aerosol once a day for 5 days; and interleukin (IL)-18 intervention group (Group C, undergoing intraperitoneally injection of OVA once and intraperitoneal injection of IL-18 on the days 1, 3, 8. 10. 15, 17, and 19. Twenty-four hours after the final spraying or IL-18 injection the bronchalveolar lavage fluid (BALF) of the left lungs were obtained. HE staining was conducted to the sediment to examine the numbers of eosinophils, neutrophils, and monocytes. ELISA was used to detect the Th1/Th2 cytokines in the BALF. The left lungs underwent pathological examination.

RESULTS

The number of EOS in BALF of Groups A was (98 +/- 58) x 10(6)/L, significantly higher than those of Group B, (12 +/- 10) x 10(6)/L, and Group C, (29 +/- 10) x 10(6)/L (P < 0.01 and P < 0.05). The numbers of neutrophils in the BALF of Group A was (24 +/- 16) x 10(6)/L, significantly higher than those of Group B and C [(9 +/- 7) x 10(6)/L and (10 +/- 5) x 10(6)/L respectively, both P < 0.05]. The concentration of IFN-gamma and IL-2 in group A were both significantly lower than those of Group B and Group C (P < 0.05 and P < 0.01). The concentration of IL-4 in Group A was significantly higher than those of Groups B and C (both P < 0.05). The concentration of IL-5 of Group A was significantly higher than those of Group Bs and C (both P < 0.01).

CONCLUSION

IL-18 effectively inhibits asthmatic airway inflammation by regulating the Th1/Th2 balance.

Authors+Show Affiliations

Chen XQ
Department of Respiratory Diseases, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Lin TY
No affiliation info available

MeSH

AnimalsAsthmaGuinea PigsInterferon-gammaInterleukin-18Interleukin-4Interleukin-5MaleOvalbuminRandom AllocationTh1 CellsTh2 Cells

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16313780

Citation

Chen, Xiang-qi, and Ting-yan Lin. "[Effects of Interleukin-18 On Asthmatic Airway Inflammation: Experimental Study of Guinea Pig Asthmatic Model]." Zhonghua Yi Xue Za Zhi, vol. 85, no. 28, 2005, pp. 1995-8.
Chen XQ, Lin TY. [Effects of interleukin-18 on asthmatic airway inflammation: experimental study of guinea pig asthmatic model]. Zhonghua Yi Xue Za Zhi. 2005;85(28):1995-8.
Chen, X. Q., & Lin, T. Y. (2005). [Effects of interleukin-18 on asthmatic airway inflammation: experimental study of guinea pig asthmatic model]. Zhonghua Yi Xue Za Zhi, 85(28), 1995-8.
Chen XQ, Lin TY. [Effects of Interleukin-18 On Asthmatic Airway Inflammation: Experimental Study of Guinea Pig Asthmatic Model]. Zhonghua Yi Xue Za Zhi. 2005 Jul 27;85(28):1995-8. PubMed PMID: 16313780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of interleukin-18 on asthmatic airway inflammation: experimental study of guinea pig asthmatic model]. AU - Chen,Xiang-qi, AU - Lin,Ting-yan, PY - 2005/11/30/pubmed PY - 2007/5/30/medline PY - 2005/11/30/entrez SP - 1995 EP - 8 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 85 IS - 28 N2 - OBJECTIVE: To investigate the effects of Interleukin-18 (IL-18) on asthmatic airway inflammation. METHODS: Thirty healthy adult male guinea pigs were randomly divided into 3 equal groups: asthmatic model group (Group A, undergoing intraperitoneally injection of ovalbumin (OVA) once and spraying of OVA aerosol once a day for 5 days; control group (Group B), undergoing intraperitoneally injection of OVA once and spraying of normal saline aerosol once a day for 5 days; and interleukin (IL)-18 intervention group (Group C, undergoing intraperitoneally injection of OVA once and intraperitoneal injection of IL-18 on the days 1, 3, 8. 10. 15, 17, and 19. Twenty-four hours after the final spraying or IL-18 injection the bronchalveolar lavage fluid (BALF) of the left lungs were obtained. HE staining was conducted to the sediment to examine the numbers of eosinophils, neutrophils, and monocytes. ELISA was used to detect the Th1/Th2 cytokines in the BALF. The left lungs underwent pathological examination. RESULTS: The number of EOS in BALF of Groups A was (98 +/- 58) x 10(6)/L, significantly higher than those of Group B, (12 +/- 10) x 10(6)/L, and Group C, (29 +/- 10) x 10(6)/L (P < 0.01 and P < 0.05). The numbers of neutrophils in the BALF of Group A was (24 +/- 16) x 10(6)/L, significantly higher than those of Group B and C [(9 +/- 7) x 10(6)/L and (10 +/- 5) x 10(6)/L respectively, both P < 0.05]. The concentration of IFN-gamma and IL-2 in group A were both significantly lower than those of Group B and Group C (P < 0.05 and P < 0.01). The concentration of IL-4 in Group A was significantly higher than those of Groups B and C (both P < 0.05). The concentration of IL-5 of Group A was significantly higher than those of Group Bs and C (both P < 0.01). CONCLUSION: IL-18 effectively inhibits asthmatic airway inflammation by regulating the Th1/Th2 balance. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/16313780/[Effects_of_interleukin_18_on_asthmatic_airway_inflammation:_experimental_study_of_guinea_pig_asthmatic_model]_ DB - PRIME DP - Unbound Medicine ER -