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Apple (Malus pumila) procyanidins fractionated according to the degree of polymerization using normal-phase chromatography and characterized by HPLC-ESI/MS and MALDI-TOF/MS.
J Chromatogr A. 2006 Jan 13; 1102(1-2):206-13.JC

Abstract

Our previously reported method for the fractionation of apple procyanidins was modified successfully to achieve the separation of (epi)catechins and procyanidins (ranging from dimers to octamers) according to the degree of polymerization. Normal-phase chromatography was employed, using a hexane-methanol-ethyl acetate mixture as the mobile phase. Each fraction was characterized using high-performance liquid-chromatography electrospray-ionization mass spectrometry (HPLC-ESI/MS) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS). This method will be useful for the evaluation of the physiological functions of proanthocyanidins and for the elucidation of their individual structures.

Authors+Show Affiliations

Fundamental Research Laboratory, Asahi Breweries, Ltd., 1-21 Midori 1-chome, Moriya, Ibaraki 302-0106, Japan. toshihiko.shoji@asahibeer.co.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16313915

Citation

Shoji, Toshihiko, et al. "Apple (Malus Pumila) Procyanidins Fractionated According to the Degree of Polymerization Using Normal-phase Chromatography and Characterized By HPLC-ESI/MS and MALDI-TOF/MS." Journal of Chromatography. A, vol. 1102, no. 1-2, 2006, pp. 206-13.
Shoji T, Masumoto S, Moriichi N, et al. Apple (Malus pumila) procyanidins fractionated according to the degree of polymerization using normal-phase chromatography and characterized by HPLC-ESI/MS and MALDI-TOF/MS. J Chromatogr A. 2006;1102(1-2):206-13.
Shoji, T., Masumoto, S., Moriichi, N., Kanda, T., & Ohtake, Y. (2006). Apple (Malus pumila) procyanidins fractionated according to the degree of polymerization using normal-phase chromatography and characterized by HPLC-ESI/MS and MALDI-TOF/MS. Journal of Chromatography. A, 1102(1-2), 206-13.
Shoji T, et al. Apple (Malus Pumila) Procyanidins Fractionated According to the Degree of Polymerization Using Normal-phase Chromatography and Characterized By HPLC-ESI/MS and MALDI-TOF/MS. J Chromatogr A. 2006 Jan 13;1102(1-2):206-13. PubMed PMID: 16313915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apple (Malus pumila) procyanidins fractionated according to the degree of polymerization using normal-phase chromatography and characterized by HPLC-ESI/MS and MALDI-TOF/MS. AU - Shoji,Toshihiko, AU - Masumoto,Saeko, AU - Moriichi,Nina, AU - Kanda,Tomomasa, AU - Ohtake,Yasuyuki, Y1 - 2005/11/28/ PY - 2005/02/16/received PY - 2005/10/17/revised PY - 2005/10/27/accepted PY - 2005/11/30/pubmed PY - 2006/3/10/medline PY - 2005/11/30/entrez SP - 206 EP - 13 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1102 IS - 1-2 N2 - Our previously reported method for the fractionation of apple procyanidins was modified successfully to achieve the separation of (epi)catechins and procyanidins (ranging from dimers to octamers) according to the degree of polymerization. Normal-phase chromatography was employed, using a hexane-methanol-ethyl acetate mixture as the mobile phase. Each fraction was characterized using high-performance liquid-chromatography electrospray-ionization mass spectrometry (HPLC-ESI/MS) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS). This method will be useful for the evaluation of the physiological functions of proanthocyanidins and for the elucidation of their individual structures. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16313915/Apple__Malus_pumila__procyanidins_fractionated_according_to_the_degree_of_polymerization_using_normal_phase_chromatography_and_characterized_by_HPLC_ESI/MS_and_MALDI_TOF/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(05)02065-0 DB - PRIME DP - Unbound Medicine ER -