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Determination of biogenic amines in chocolate by ion chromatographic separation and pulsed integrated amperometric detection with implemented wave-form at Au disposable electrode.
J Chromatogr A 2005; 1098(1-2):111-5JC

Abstract

A rapid and selective cation exchange chromatographic method coupled to integrated pulsed amperometric detection (PAD) has been developed to quantify biogenic amines in chocolate. The method is based on gradient elution of aqueous methanesulfonic acid with post column addition of strong base to obtain suitable conditions for amperometric detection. A potential waveform able to keep long time performance of the Au disposable electrode was set up. Total analysis time is less than 20min. Concentration levels of dopamine, serotonin, tyramine, histamine and 2-phenylethylamine were measured, after extraction with perchloric acid from 2g samples previously defatted twice with petroleum ether. The method was used to determine the analytes in chocolate real matrices and their quantification was made with standard addition method. Only dopamine, histamine and serotonin were found in the analysed real samples. Repeatabilities of their signals, computed on their amounts in the real samples, were 5% for all of them. Repeatabilities of tyramine and phenethylamine were relative to standard additions to real samples (close to 1mg/l in the extract) and were 7 and 3%, respectively. Detection limits were computed with the 3s of the baseline noise combined with the calibration plot regression parameters. They were satisfactorily low for all amines: 3mg/kg for dopamine, 2mg/kg for tyramine, 1mg/kg for histamine, 2mg/kg for serotonin, 3mg/kg for 2-phenylethylamine.

Authors+Show Affiliations

Department of Chemical Sciences, University of Padua, via Marzolo 1, I-35131 Padova, Italy. paolo.pastore@unipd.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16314166

Citation

Pastore, Paolo, et al. "Determination of Biogenic Amines in Chocolate By Ion Chromatographic Separation and Pulsed Integrated Amperometric Detection With Implemented Wave-form at Au Disposable Electrode." Journal of Chromatography. A, vol. 1098, no. 1-2, 2005, pp. 111-5.
Pastore P, Favaro G, Badocco D, et al. Determination of biogenic amines in chocolate by ion chromatographic separation and pulsed integrated amperometric detection with implemented wave-form at Au disposable electrode. J Chromatogr A. 2005;1098(1-2):111-5.
Pastore, P., Favaro, G., Badocco, D., Tapparo, A., Cavalli, S., & Saccani, G. (2005). Determination of biogenic amines in chocolate by ion chromatographic separation and pulsed integrated amperometric detection with implemented wave-form at Au disposable electrode. Journal of Chromatography. A, 1098(1-2), pp. 111-5.
Pastore P, et al. Determination of Biogenic Amines in Chocolate By Ion Chromatographic Separation and Pulsed Integrated Amperometric Detection With Implemented Wave-form at Au Disposable Electrode. J Chromatogr A. 2005 Dec 9;1098(1-2):111-5. PubMed PMID: 16314166.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of biogenic amines in chocolate by ion chromatographic separation and pulsed integrated amperometric detection with implemented wave-form at Au disposable electrode. AU - Pastore,Paolo, AU - Favaro,Gabriella, AU - Badocco,Denis, AU - Tapparo,Andrea, AU - Cavalli,Silvano, AU - Saccani,Giovanna, Y1 - 2005/09/08/ PY - 2005/05/19/received PY - 2005/08/09/revised PY - 2005/08/23/accepted PY - 2005/11/30/pubmed PY - 2006/2/24/medline PY - 2005/11/30/entrez SP - 111 EP - 5 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1098 IS - 1-2 N2 - A rapid and selective cation exchange chromatographic method coupled to integrated pulsed amperometric detection (PAD) has been developed to quantify biogenic amines in chocolate. The method is based on gradient elution of aqueous methanesulfonic acid with post column addition of strong base to obtain suitable conditions for amperometric detection. A potential waveform able to keep long time performance of the Au disposable electrode was set up. Total analysis time is less than 20min. Concentration levels of dopamine, serotonin, tyramine, histamine and 2-phenylethylamine were measured, after extraction with perchloric acid from 2g samples previously defatted twice with petroleum ether. The method was used to determine the analytes in chocolate real matrices and their quantification was made with standard addition method. Only dopamine, histamine and serotonin were found in the analysed real samples. Repeatabilities of their signals, computed on their amounts in the real samples, were 5% for all of them. Repeatabilities of tyramine and phenethylamine were relative to standard additions to real samples (close to 1mg/l in the extract) and were 7 and 3%, respectively. Detection limits were computed with the 3s of the baseline noise combined with the calibration plot regression parameters. They were satisfactorily low for all amines: 3mg/kg for dopamine, 2mg/kg for tyramine, 1mg/kg for histamine, 2mg/kg for serotonin, 3mg/kg for 2-phenylethylamine. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16314166/Determination_of_biogenic_amines_in_chocolate_by_ion_chromatographic_separation_and_pulsed_integrated_amperometric_detection_with_implemented_wave_form_at_Au_disposable_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(05)01713-9 DB - PRIME DP - Unbound Medicine ER -