Tags

Type your tag names separated by a space and hit enter

Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis.
Plant Cell. 2006 Jan; 18(1):225-42.PC

Abstract

Fungal chitin synthases (CHSs) form fibers of the cell wall and are crucial for substrate invasion and pathogenicity. Filamentous fungi contain up to 10 CHSs, which might reflect redundant functions or the complex biology of these fungi. Here, we investigate the complete repertoire of eight CHSs in the dimorphic plant pathogen Ustilago maydis. We demonstrate that all CHSs are expressed in yeast cells and hyphae. Green fluorescent protein (GFP) fusions to all CHSs localize to septa, whereas Chs5-GFP, Chs6-GFP, Chs7-yellow fluorescent protein (YFP), and Myosin chitin synthase1 (Mcs1)-YFP were found at growth regions of yeast-like cells and hyphae, indicating that they participate in tip growth. However, only the class IV CHS genes chs7 and chs5 are crucial for shaping yeast cells and hyphae ex planta. Although most CHS mutants were attenuated in plant pathogenicity, Deltachs6, Deltachs7, and Deltamcs1 mutants were drastically reduced in virulence. Deltamcs1 showed no morphological defects in hyphae, but Mcs1 became essential during invasion of the plant epidermis. Deltamcs1 hyphae entered the plant but immediately lost growth polarity and formed large aggregates of spherical cells. Our data show that the polar class IV CHSs are essential for morphogenesis ex planta, whereas the class V myosin-CHS is essential during plant infection.

Authors+Show Affiliations

Max-Planck-Institut für Terrestrische Mikrobiologie, Marburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16314447

Citation

Weber, Isabella, et al. "Polar Localizing Class V Myosin Chitin Synthases Are Essential During Early Plant Infection in the Plant Pathogenic Fungus Ustilago Maydis." The Plant Cell, vol. 18, no. 1, 2006, pp. 225-42.
Weber I, Assmann D, Thines E, et al. Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis. Plant Cell. 2006;18(1):225-42.
Weber, I., Assmann, D., Thines, E., & Steinberg, G. (2006). Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis. The Plant Cell, 18(1), 225-42.
Weber I, et al. Polar Localizing Class V Myosin Chitin Synthases Are Essential During Early Plant Infection in the Plant Pathogenic Fungus Ustilago Maydis. Plant Cell. 2006;18(1):225-42. PubMed PMID: 16314447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis. AU - Weber,Isabella, AU - Assmann,Daniela, AU - Thines,Eckhard, AU - Steinberg,Gero, Y1 - 2005/11/28/ PY - 2005/11/30/pubmed PY - 2006/4/18/medline PY - 2005/11/30/entrez SP - 225 EP - 42 JF - The Plant cell JO - Plant Cell VL - 18 IS - 1 N2 - Fungal chitin synthases (CHSs) form fibers of the cell wall and are crucial for substrate invasion and pathogenicity. Filamentous fungi contain up to 10 CHSs, which might reflect redundant functions or the complex biology of these fungi. Here, we investigate the complete repertoire of eight CHSs in the dimorphic plant pathogen Ustilago maydis. We demonstrate that all CHSs are expressed in yeast cells and hyphae. Green fluorescent protein (GFP) fusions to all CHSs localize to septa, whereas Chs5-GFP, Chs6-GFP, Chs7-yellow fluorescent protein (YFP), and Myosin chitin synthase1 (Mcs1)-YFP were found at growth regions of yeast-like cells and hyphae, indicating that they participate in tip growth. However, only the class IV CHS genes chs7 and chs5 are crucial for shaping yeast cells and hyphae ex planta. Although most CHS mutants were attenuated in plant pathogenicity, Deltachs6, Deltachs7, and Deltamcs1 mutants were drastically reduced in virulence. Deltamcs1 showed no morphological defects in hyphae, but Mcs1 became essential during invasion of the plant epidermis. Deltamcs1 hyphae entered the plant but immediately lost growth polarity and formed large aggregates of spherical cells. Our data show that the polar class IV CHSs are essential for morphogenesis ex planta, whereas the class V myosin-CHS is essential during plant infection. SN - 1040-4651 UR - https://www.unboundmedicine.com/medline/citation/16314447/Polar_localizing_class_V_myosin_chitin_synthases_are_essential_during_early_plant_infection_in_the_plant_pathogenic_fungus_Ustilago_maydis_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=16314447 DB - PRIME DP - Unbound Medicine ER -