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Modulation of density-fractionated RBC deformability by nitric oxide.
Clin Hemorheol Microcirc. 2005; 33(4):363-7.CH

Abstract

The role of nitric oxide (NO) in maintaining normal mechanical behavior of red blood cell (RBC) has been previously demonstrated. The effects of NO donor and NOS inhibitor on the mechanical properties of density fractionated RBC were tested in this study. A non-specific NOS inhibitor, N-omega-nitro-L-arginine methyl ester (L-NAME) at a concentration of 10(-3) M and sodium nitroprusside (SNP), a nitric oxide donor at a concentration of 10(-6) M was added to blood samples with hematocrit adjusted to 0.4 l/l and RBC deformability was measured by an ektacytometer in the density fractionated RBC after one hour incubation at 37 degrees C. There was no significant effect of the NO donor SNP on cellular deformability in the older (denser) RBC fraction in contrast with the younger (least dense) fraction. Alternatively, the sensitivity of cellular deformability to competitive NOS inhibition by L-NAME was greater in the older fraction. These findings suggest that older RBC are characterized by diminished internal NO synthesis and are also less sensitive to external NO indicating that the target mechanisms for NO may also be deteriorated.

Authors+Show Affiliations

Department of Physiology, Pamukkale University Faculty of Medicine, Denizli, Turkey.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16317245

Citation

Bor-Kucukatay, Melek, et al. "Modulation of Density-fractionated RBC Deformability By Nitric Oxide." Clinical Hemorheology and Microcirculation, vol. 33, no. 4, 2005, pp. 363-7.
Bor-Kucukatay M, Meiselman HJ, Başkurt OK. Modulation of density-fractionated RBC deformability by nitric oxide. Clin Hemorheol Microcirc. 2005;33(4):363-7.
Bor-Kucukatay, M., Meiselman, H. J., & Başkurt, O. K. (2005). Modulation of density-fractionated RBC deformability by nitric oxide. Clinical Hemorheology and Microcirculation, 33(4), 363-7.
Bor-Kucukatay M, Meiselman HJ, Başkurt OK. Modulation of Density-fractionated RBC Deformability By Nitric Oxide. Clin Hemorheol Microcirc. 2005;33(4):363-7. PubMed PMID: 16317245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of density-fractionated RBC deformability by nitric oxide. AU - Bor-Kucukatay,Melek, AU - Meiselman,Herbert J, AU - Başkurt,Oguz K, PY - 2005/12/1/pubmed PY - 2006/4/21/medline PY - 2005/12/1/entrez SP - 363 EP - 7 JF - Clinical hemorheology and microcirculation JO - Clin Hemorheol Microcirc VL - 33 IS - 4 N2 - The role of nitric oxide (NO) in maintaining normal mechanical behavior of red blood cell (RBC) has been previously demonstrated. The effects of NO donor and NOS inhibitor on the mechanical properties of density fractionated RBC were tested in this study. A non-specific NOS inhibitor, N-omega-nitro-L-arginine methyl ester (L-NAME) at a concentration of 10(-3) M and sodium nitroprusside (SNP), a nitric oxide donor at a concentration of 10(-6) M was added to blood samples with hematocrit adjusted to 0.4 l/l and RBC deformability was measured by an ektacytometer in the density fractionated RBC after one hour incubation at 37 degrees C. There was no significant effect of the NO donor SNP on cellular deformability in the older (denser) RBC fraction in contrast with the younger (least dense) fraction. Alternatively, the sensitivity of cellular deformability to competitive NOS inhibition by L-NAME was greater in the older fraction. These findings suggest that older RBC are characterized by diminished internal NO synthesis and are also less sensitive to external NO indicating that the target mechanisms for NO may also be deteriorated. SN - 1386-0291 UR - https://www.unboundmedicine.com/medline/citation/16317245/Modulation_of_density_fractionated_RBC_deformability_by_nitric_oxide_ DB - PRIME DP - Unbound Medicine ER -