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The effect of lactic acid on anaerobic carbon or nitrogen limited chemostat cultures of Saccharomyces cerevisiae.
Appl Microbiol Biotechnol. 2006 Jul; 71(4):533-42.AM

Abstract

Weak organic acids are well-known metabolic effectors in yeast and other micro-organisms. High concentrations of lactic acid due to infection of lactic acid bacteria often occurs in combination with growth under nutrient-limiting conditions in industrial yeast fermentations. The effects of lactic acid on growth and product formation of Saccharomyces cerevisiae were studied, with cells growing under carbon- or nitrogen-limiting conditions in anaerobic chemostat cultures (D=0.1 h(-1)) at pH values 3.25 and 5. It was shown that lactic acid in industrially relevant concentrations had a rather limited effect on the metabolism of S. cerevisiae. However, there was an effect on the energetic status of the cells, i.e. lactic acid addition provoked a reduction in the adenosine triphosphate (ATP) content of the cells. The decrease in ATP was not accompanied by a significant increase in the adenosine monophosphate levels.

Authors+Show Affiliations

Department of Chemistry and Bioscience, Molecular Biotechnology, Lundberg Laboratory, Chalmers University of Technology, Box 462, 405 30, Gothenburg, Sweden. elisabeth.thomsson@chembio.chalmers.seNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16317544

Citation

Thomsson, Elisabeth, and Christer Larsson. "The Effect of Lactic Acid On Anaerobic Carbon or Nitrogen Limited Chemostat Cultures of Saccharomyces Cerevisiae." Applied Microbiology and Biotechnology, vol. 71, no. 4, 2006, pp. 533-42.
Thomsson E, Larsson C. The effect of lactic acid on anaerobic carbon or nitrogen limited chemostat cultures of Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2006;71(4):533-42.
Thomsson, E., & Larsson, C. (2006). The effect of lactic acid on anaerobic carbon or nitrogen limited chemostat cultures of Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 71(4), 533-42.
Thomsson E, Larsson C. The Effect of Lactic Acid On Anaerobic Carbon or Nitrogen Limited Chemostat Cultures of Saccharomyces Cerevisiae. Appl Microbiol Biotechnol. 2006;71(4):533-42. PubMed PMID: 16317544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of lactic acid on anaerobic carbon or nitrogen limited chemostat cultures of Saccharomyces cerevisiae. AU - Thomsson,Elisabeth, AU - Larsson,Christer, Y1 - 2005/10/27/ PY - 2005/07/06/received PY - 2005/09/17/accepted PY - 2005/09/13/revised PY - 2005/12/1/pubmed PY - 2006/10/13/medline PY - 2005/12/1/entrez SP - 533 EP - 42 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 71 IS - 4 N2 - Weak organic acids are well-known metabolic effectors in yeast and other micro-organisms. High concentrations of lactic acid due to infection of lactic acid bacteria often occurs in combination with growth under nutrient-limiting conditions in industrial yeast fermentations. The effects of lactic acid on growth and product formation of Saccharomyces cerevisiae were studied, with cells growing under carbon- or nitrogen-limiting conditions in anaerobic chemostat cultures (D=0.1 h(-1)) at pH values 3.25 and 5. It was shown that lactic acid in industrially relevant concentrations had a rather limited effect on the metabolism of S. cerevisiae. However, there was an effect on the energetic status of the cells, i.e. lactic acid addition provoked a reduction in the adenosine triphosphate (ATP) content of the cells. The decrease in ATP was not accompanied by a significant increase in the adenosine monophosphate levels. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/16317544/The_effect_of_lactic_acid_on_anaerobic_carbon_or_nitrogen_limited_chemostat_cultures_of_Saccharomyces_cerevisiae_ L2 - https://dx.doi.org/10.1007/s00253-005-0195-3 DB - PRIME DP - Unbound Medicine ER -