Tags

Type your tag names separated by a space and hit enter

Polyphenolic apple juice extracts and their major constituents reduce oxidative damage in human colon cell lines.
Mol Nutr Food Res. 2006 Jan; 50(1):24-33.MN

Abstract

Apple juice containing high amounts of antioxidative polyphenols might protect the intestine against oxidative cell damage. We investigated the preventive effectiveness of polyphenolic juice extracts of different origins (cider and table apples) in comparison to their major constituents in human colon cell lines (Caco-2, HT29). Parameters studied were (oxidative) DNA damage (Comet assay), glutathione level (photometric kinetic assay), cellular redox status (dichlorofluorescein assay) and antioxidant capacity. The extracts (50-250 microg/mL) modulated DNA damage and redox status in a concentration-dependent manner at 24-h incubation. The pomace extraction technology, applied for juice preparation, and the preferential selection of cider apple varieties influenced the polyphenolic pattern and increased the biological effectiveness of the extracts. The preventive potential of major juice constituents (1-100 microM, 24 h) strongly differed: rutin, epicatechin and caffeic acid clearly reduced (oxidative) DNA damage (Caco-2), chlorogenic acid efficiently decreased cellular reactive oxygen species level (HT29, Caco-2). The aglyca quercetin and phloretin exhibited the highest preventive/antioxidant capacity in all assays. The stability of the compounds inversely correlated with their preventive effectiveness and might contribute to the observed cell specific sensitivities. In conclusion, apple juice extracts distinctly reduce oxidative cell damage in human colon cell lines, an effect, which in part can be accounted for by their major constituents.

Authors+Show Affiliations

Department of Chemistry, Division of Food Chemistry and Environmental Toxicology, University of Kaiserslautern, Kaiserslautern, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16317784

Citation

Schaefer, Sandra, et al. "Polyphenolic Apple Juice Extracts and Their Major Constituents Reduce Oxidative Damage in Human Colon Cell Lines." Molecular Nutrition & Food Research, vol. 50, no. 1, 2006, pp. 24-33.
Schaefer S, Baum M, Eisenbrand G, et al. Polyphenolic apple juice extracts and their major constituents reduce oxidative damage in human colon cell lines. Mol Nutr Food Res. 2006;50(1):24-33.
Schaefer, S., Baum, M., Eisenbrand, G., Dietrich, H., Will, F., & Janzowski, C. (2006). Polyphenolic apple juice extracts and their major constituents reduce oxidative damage in human colon cell lines. Molecular Nutrition & Food Research, 50(1), 24-33.
Schaefer S, et al. Polyphenolic Apple Juice Extracts and Their Major Constituents Reduce Oxidative Damage in Human Colon Cell Lines. Mol Nutr Food Res. 2006;50(1):24-33. PubMed PMID: 16317784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenolic apple juice extracts and their major constituents reduce oxidative damage in human colon cell lines. AU - Schaefer,Sandra, AU - Baum,Matthias, AU - Eisenbrand,Gerhard, AU - Dietrich,Helmut, AU - Will,Frank, AU - Janzowski,Christine, PY - 2005/12/1/pubmed PY - 2006/6/20/medline PY - 2005/12/1/entrez SP - 24 EP - 33 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 50 IS - 1 N2 - Apple juice containing high amounts of antioxidative polyphenols might protect the intestine against oxidative cell damage. We investigated the preventive effectiveness of polyphenolic juice extracts of different origins (cider and table apples) in comparison to their major constituents in human colon cell lines (Caco-2, HT29). Parameters studied were (oxidative) DNA damage (Comet assay), glutathione level (photometric kinetic assay), cellular redox status (dichlorofluorescein assay) and antioxidant capacity. The extracts (50-250 microg/mL) modulated DNA damage and redox status in a concentration-dependent manner at 24-h incubation. The pomace extraction technology, applied for juice preparation, and the preferential selection of cider apple varieties influenced the polyphenolic pattern and increased the biological effectiveness of the extracts. The preventive potential of major juice constituents (1-100 microM, 24 h) strongly differed: rutin, epicatechin and caffeic acid clearly reduced (oxidative) DNA damage (Caco-2), chlorogenic acid efficiently decreased cellular reactive oxygen species level (HT29, Caco-2). The aglyca quercetin and phloretin exhibited the highest preventive/antioxidant capacity in all assays. The stability of the compounds inversely correlated with their preventive effectiveness and might contribute to the observed cell specific sensitivities. In conclusion, apple juice extracts distinctly reduce oxidative cell damage in human colon cell lines, an effect, which in part can be accounted for by their major constituents. SN - 1613-4125 UR - https://www.unboundmedicine.com/medline/citation/16317784/Polyphenolic_apple_juice_extracts_and_their_major_constituents_reduce_oxidative_damage_in_human_colon_cell_lines_ L2 - https://doi.org/10.1002/mnfr.200500136 DB - PRIME DP - Unbound Medicine ER -