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Enantioseparation of baclofen with highly sulfated beta-cyclodextrin by capillary electrophoresis with laser-induced fluorescence detection.
J Sep Sci. 2005 Nov; 28(16):2187-92.JS

Abstract

The enantioseparation of baclofen (4-amino-3-p-chlorophenylbutyric acid) was achieved by CE-LIF with highly sulfated beta-CD (HS-beta-CD) as chiral selector. Naphthalene-2,3-dicarboxaldehyde was used for the derivatization of nonfluorescent baclofen. HS-beta-CD (2%) containing 50 mM borate buffer at pH 9.5 was chosen as the optimal running electrolyte and applied to the analysis of baclofen enantiomers in human plasma. The linearity of calibration curves (R2 > or = 0.998) for R-(-) and S-(+)-baclofen was in the 0.1-2.0 microM concentration range. After a simple ACN-protein precipitation, the LOD of baclofen in plasma sample was found as low as 50 nM.

Authors+Show Affiliations

Kavran-Belin G
Laboratory of Mass Spectrometry, University of Geneva, Geneva, Switzerland. Gamze.Belin@chiphy.unige.ch
Rudaz S
No affiliation info available
Veuthey JL
No affiliation info available

MeSH

BaclofenElectrophoresis, CapillaryEstranesEthanolFluorometryHumansIsomerismLasersMethanolNitrilesPlasmabeta-Cyclodextrins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16318216

Citation

Kavran-Belin, Gamze, et al. "Enantioseparation of Baclofen With Highly Sulfated Beta-cyclodextrin By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Separation Science, vol. 28, no. 16, 2005, pp. 2187-92.
Kavran-Belin G, Rudaz S, Veuthey JL. Enantioseparation of baclofen with highly sulfated beta-cyclodextrin by capillary electrophoresis with laser-induced fluorescence detection. J Sep Sci. 2005;28(16):2187-92.
Kavran-Belin, G., Rudaz, S., & Veuthey, J. L. (2005). Enantioseparation of baclofen with highly sulfated beta-cyclodextrin by capillary electrophoresis with laser-induced fluorescence detection. Journal of Separation Science, 28(16), 2187-92.
Kavran-Belin G, Rudaz S, Veuthey JL. Enantioseparation of Baclofen With Highly Sulfated Beta-cyclodextrin By Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Sep Sci. 2005;28(16):2187-92. PubMed PMID: 16318216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantioseparation of baclofen with highly sulfated beta-cyclodextrin by capillary electrophoresis with laser-induced fluorescence detection. AU - Kavran-Belin,Gamze, AU - Rudaz,Serge, AU - Veuthey,Jean-Luc, PY - 2005/12/2/pubmed PY - 2008/2/2/medline PY - 2005/12/2/entrez SP - 2187 EP - 92 JF - Journal of separation science JO - J Sep Sci VL - 28 IS - 16 N2 - The enantioseparation of baclofen (4-amino-3-p-chlorophenylbutyric acid) was achieved by CE-LIF with highly sulfated beta-CD (HS-beta-CD) as chiral selector. Naphthalene-2,3-dicarboxaldehyde was used for the derivatization of nonfluorescent baclofen. HS-beta-CD (2%) containing 50 mM borate buffer at pH 9.5 was chosen as the optimal running electrolyte and applied to the analysis of baclofen enantiomers in human plasma. The linearity of calibration curves (R2 > or = 0.998) for R-(-) and S-(+)-baclofen was in the 0.1-2.0 microM concentration range. After a simple ACN-protein precipitation, the LOD of baclofen in plasma sample was found as low as 50 nM. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/16318216/Enantioseparation_of_baclofen_with_highly_sulfated_beta_cyclodextrin_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -

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