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CTX-M-15 extended-spectrum (beta)-lactamase from Nigerian Klebsiella pneumoniae.
J Antimicrob Chemother. 2006 Jan; 57(1):24-30.JA

Abstract

OBJECTIVES

In this study, extended-spectrum beta-lactamases (ESBLs) were characterized from 30 selected multidrug-resistant Klebsiella pneumoniae strains isolated from patients with community-acquired urinary tract infections from Southwest Nigeria.

METHODS

The beta-lactamases were phenotypically characterized using isoelectric focusing, genotypically characterized using PCR assays and hybridization of the PCR products. Two of the bla(CTX-M) genes were completely sequenced. The location of the CTX-M-type genes was determined using transformation, DNA-DNA hybridization, PCR assays and hybridization of the PCR products from the Escherichia coli transformants.

RESULTS

All 30 isolates produced at least one beta-lactamase. Seventeen of the isolates were resistant to cefotaxime, and had > or =100-fold reduction in susceptibility with cefotaxime plus clavulanic acid (4 mg/L), indicating the presence of an ESBL. The 17 isolates were shown to have bla(CTX-M) genes that were associated with large plasmids (> or =58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from previously sequenced CTX-M-15 beta-lactamases. The ISEcp1 element was located upstream of bla(CTX-M-15) in the same position as previously described. In addition, 23 of the isolates produced TEM beta-lactamases, 27 produced SHV beta-lactamases and four produced AmpC beta-lactamases.

CONCLUSIONS

Thirty K. pneumoniae produced multiple beta-lactamases, with 57% producing CTX-M enzymes. This is the first characterization of CTX-M-15-positive K. pneumoniae in Western Africa.

Authors+Show Affiliations

Department of Pharmaceutical Microbiology, University of Ibadan, Ibadan, Nigeria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16319181

Citation

Soge, Olusegun O., et al. "CTX-M-15 Extended-spectrum (beta)-lactamase From Nigerian Klebsiella Pneumoniae." The Journal of Antimicrobial Chemotherapy, vol. 57, no. 1, 2006, pp. 24-30.
Soge OO, Queenan AM, Ojo KK, et al. CTX-M-15 extended-spectrum (beta)-lactamase from Nigerian Klebsiella pneumoniae. J Antimicrob Chemother. 2006;57(1):24-30.
Soge, O. O., Queenan, A. M., Ojo, K. K., Adeniyi, B. A., & Roberts, M. C. (2006). CTX-M-15 extended-spectrum (beta)-lactamase from Nigerian Klebsiella pneumoniae. The Journal of Antimicrobial Chemotherapy, 57(1), 24-30.
Soge OO, et al. CTX-M-15 Extended-spectrum (beta)-lactamase From Nigerian Klebsiella Pneumoniae. J Antimicrob Chemother. 2006;57(1):24-30. PubMed PMID: 16319181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CTX-M-15 extended-spectrum (beta)-lactamase from Nigerian Klebsiella pneumoniae. AU - Soge,Olusegun O, AU - Queenan,Anne Marie, AU - Ojo,Kayode K, AU - Adeniyi,Bolanle A, AU - Roberts,Marilyn C, Y1 - 2005/11/30/ PY - 2005/12/2/pubmed PY - 2006/3/22/medline PY - 2005/12/2/entrez SP - 24 EP - 30 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 57 IS - 1 N2 - OBJECTIVES: In this study, extended-spectrum beta-lactamases (ESBLs) were characterized from 30 selected multidrug-resistant Klebsiella pneumoniae strains isolated from patients with community-acquired urinary tract infections from Southwest Nigeria. METHODS: The beta-lactamases were phenotypically characterized using isoelectric focusing, genotypically characterized using PCR assays and hybridization of the PCR products. Two of the bla(CTX-M) genes were completely sequenced. The location of the CTX-M-type genes was determined using transformation, DNA-DNA hybridization, PCR assays and hybridization of the PCR products from the Escherichia coli transformants. RESULTS: All 30 isolates produced at least one beta-lactamase. Seventeen of the isolates were resistant to cefotaxime, and had > or =100-fold reduction in susceptibility with cefotaxime plus clavulanic acid (4 mg/L), indicating the presence of an ESBL. The 17 isolates were shown to have bla(CTX-M) genes that were associated with large plasmids (> or =58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from previously sequenced CTX-M-15 beta-lactamases. The ISEcp1 element was located upstream of bla(CTX-M-15) in the same position as previously described. In addition, 23 of the isolates produced TEM beta-lactamases, 27 produced SHV beta-lactamases and four produced AmpC beta-lactamases. CONCLUSIONS: Thirty K. pneumoniae produced multiple beta-lactamases, with 57% producing CTX-M enzymes. This is the first characterization of CTX-M-15-positive K. pneumoniae in Western Africa. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/16319181/CTX_M_15_extended_spectrum__beta__lactamase_from_Nigerian_Klebsiella_pneumoniae_ DB - PRIME DP - Unbound Medicine ER -