Tags

Type your tag names separated by a space and hit enter

Apparent absence of atovaquone/proguanil resistance in 477 Plasmodium falciparum isolates from untreated French travellers.
J Antimicrob Chemother. 2006 Jan; 57(1):110-5.JA

Abstract

OBJECTIVES

We examined the atovaquone in vitro susceptibility and the cytochrome b (cytb) gene polymorphism of African Plasmodium falciparum isolates during the first years of atovaquone/proguanil use.

PATIENTS AND METHODS

Between 1999 and 2004, we collected blood samples from French P. falciparum-infected patients returning from African countries. Atovaquone susceptibility was determined using an in vitro isotopic test and cytb genotyping was performed by restriction fragment length polymorphism analysis and sequencing. These results were analysed according to the clinical response to atovaquone/proguanil treatment.

RESULTS

No in vitro atovaquone resistance (IC50 > 1900 nM) and no cytb mutation leading to the Y268S substitution were detected among 477 unexposed African P. falciparum isolates. Eight cytb polymorphisms were found outside the ubiquinone reduction site by sequencing the entire gene of 270 isolates. One atovaquone/proguanil treatment failure was documented; the post-treatment isolate had an atovaquone susceptibility of 8230 nM and the Ser268 Cytb change; the pre-treatment isolate, obtained 4 weeks previously, was Cytb Tyr268 (wild-type).

CONCLUSIONS

No atovaquone/proguanil resistance was detected by phenotyping or genotyping among 477 unexposed African P. falciparum isolates. Atovaquone/proguanil-resistant parasite was detectable only in the post-treatment isolate from a treatment failure.

Authors+Show Affiliations

Centre National de Référence pour la Chimiosensibilité du Paludisme, APHP, Hôpital Bichat-Claude Bernard, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16319183

Citation

Musset, Lise, et al. "Apparent Absence of Atovaquone/proguanil Resistance in 477 Plasmodium Falciparum Isolates From Untreated French Travellers." The Journal of Antimicrobial Chemotherapy, vol. 57, no. 1, 2006, pp. 110-5.
Musset L, Pradines B, Parzy D, et al. Apparent absence of atovaquone/proguanil resistance in 477 Plasmodium falciparum isolates from untreated French travellers. J Antimicrob Chemother. 2006;57(1):110-5.
Musset, L., Pradines, B., Parzy, D., Durand, R., Bigot, P., & Le Bras, J. (2006). Apparent absence of atovaquone/proguanil resistance in 477 Plasmodium falciparum isolates from untreated French travellers. The Journal of Antimicrobial Chemotherapy, 57(1), 110-5.
Musset L, et al. Apparent Absence of Atovaquone/proguanil Resistance in 477 Plasmodium Falciparum Isolates From Untreated French Travellers. J Antimicrob Chemother. 2006;57(1):110-5. PubMed PMID: 16319183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apparent absence of atovaquone/proguanil resistance in 477 Plasmodium falciparum isolates from untreated French travellers. AU - Musset,Lise, AU - Pradines,Bruno, AU - Parzy,Daniel, AU - Durand,Rémy, AU - Bigot,Patricia, AU - Le Bras,Jacques, Y1 - 2005/11/30/ PY - 2005/12/2/pubmed PY - 2006/3/22/medline PY - 2005/12/2/entrez SP - 110 EP - 5 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 57 IS - 1 N2 - OBJECTIVES: We examined the atovaquone in vitro susceptibility and the cytochrome b (cytb) gene polymorphism of African Plasmodium falciparum isolates during the first years of atovaquone/proguanil use. PATIENTS AND METHODS: Between 1999 and 2004, we collected blood samples from French P. falciparum-infected patients returning from African countries. Atovaquone susceptibility was determined using an in vitro isotopic test and cytb genotyping was performed by restriction fragment length polymorphism analysis and sequencing. These results were analysed according to the clinical response to atovaquone/proguanil treatment. RESULTS: No in vitro atovaquone resistance (IC50 > 1900 nM) and no cytb mutation leading to the Y268S substitution were detected among 477 unexposed African P. falciparum isolates. Eight cytb polymorphisms were found outside the ubiquinone reduction site by sequencing the entire gene of 270 isolates. One atovaquone/proguanil treatment failure was documented; the post-treatment isolate had an atovaquone susceptibility of 8230 nM and the Ser268 Cytb change; the pre-treatment isolate, obtained 4 weeks previously, was Cytb Tyr268 (wild-type). CONCLUSIONS: No atovaquone/proguanil resistance was detected by phenotyping or genotyping among 477 unexposed African P. falciparum isolates. Atovaquone/proguanil-resistant parasite was detectable only in the post-treatment isolate from a treatment failure. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/16319183/Apparent_absence_of_atovaquone/proguanil_resistance_in_477_Plasmodium_falciparum_isolates_from_untreated_French_travellers_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dki420 DB - PRIME DP - Unbound Medicine ER -