Tags

Type your tag names separated by a space and hit enter

Synthesis and metabolism of thyroid hormones is preferentially maintained in selenium-deficient transgenic mice.
Endocrinology 2006; 147(3):1306-13E

Abstract

The thyroid gland is rich in selenium (Se) and expresses a variety of selenoproteins that are involved in antioxidative defense and metabolism of thyroid hormones (TH). Se deficiency impairs regular synthesis of selenoproteins and adequate TH metabolism. We recently generated mice that lack the plasma Se carrier, selenoprotein P (SePP). SePP-knockout mice display decreased serum Se levels and manifest growth defects and neurological abnormalities partly reminiscent of thyroid gland dysfunction or profound hypothyroidism. Thus, we probed the TH axis in developing and adult SePP-knockout mice. Surprisingly, expression of Se-dependent 5'-deiodinase type 1 was only slightly altered in liver, kidney, or thyroid at postnatal d 60, and 5'-deiodinase type 2 activity in brain was normal in SePP-knockout mice. Thyroid gland morphology, thyroid glutathione peroxidase activity, thyroid Se concentration, and serum levels of TSH, T4, or T3 were within normal range. Pituitary TSHbeta transcripts and hepatic 5'-deiodinase type 1 mRNA levels were unchanged, indicating regular T3 bioactivity in thyrotropes and hepatocytes. Cerebellar granule cell migration as a sensitive indicator of local T3 action during development was undisturbed. Collectively, these findings demonstrate that low levels of serum Se or SePP in the absence of other challenges do not necessarily interfere with regular functioning of the TH axis. 5'-deiodinase isozymes are preferentially supplied, and Se-dependent enzymes in the thyroid are even less-dependent on serum levels of Se or SePP than in brain. This indicates a top priority of the thyroid gland and its selenoenzymes with respect to the hierarchical Se supply within the organism.

Authors+Show Affiliations

Institut für Experimentelle Endokrinologie, Charité-Universitaetsmedizin Berlin, Schumannstrasse 20/21, D-10117 Berlin, Germany. lutz.schomburg@charite.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16322066

Citation

Schomburg, Lutz, et al. "Synthesis and Metabolism of Thyroid Hormones Is Preferentially Maintained in Selenium-deficient Transgenic Mice." Endocrinology, vol. 147, no. 3, 2006, pp. 1306-13.
Schomburg L, Riese C, Michaelis M, et al. Synthesis and metabolism of thyroid hormones is preferentially maintained in selenium-deficient transgenic mice. Endocrinology. 2006;147(3):1306-13.
Schomburg, L., Riese, C., Michaelis, M., Griebert, E., Klein, M. O., Sapin, R., ... Köhrle, J. (2006). Synthesis and metabolism of thyroid hormones is preferentially maintained in selenium-deficient transgenic mice. Endocrinology, 147(3), pp. 1306-13.
Schomburg L, et al. Synthesis and Metabolism of Thyroid Hormones Is Preferentially Maintained in Selenium-deficient Transgenic Mice. Endocrinology. 2006;147(3):1306-13. PubMed PMID: 16322066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and metabolism of thyroid hormones is preferentially maintained in selenium-deficient transgenic mice. AU - Schomburg,Lutz, AU - Riese,Cornelia, AU - Michaelis,Marten, AU - Griebert,Emine, AU - Klein,Marc O, AU - Sapin,Remy, AU - Schweizer,Ulrich, AU - Köhrle,Josef, Y1 - 2005/12/01/ PY - 2005/12/3/pubmed PY - 2006/4/14/medline PY - 2005/12/3/entrez SP - 1306 EP - 13 JF - Endocrinology JO - Endocrinology VL - 147 IS - 3 N2 - The thyroid gland is rich in selenium (Se) and expresses a variety of selenoproteins that are involved in antioxidative defense and metabolism of thyroid hormones (TH). Se deficiency impairs regular synthesis of selenoproteins and adequate TH metabolism. We recently generated mice that lack the plasma Se carrier, selenoprotein P (SePP). SePP-knockout mice display decreased serum Se levels and manifest growth defects and neurological abnormalities partly reminiscent of thyroid gland dysfunction or profound hypothyroidism. Thus, we probed the TH axis in developing and adult SePP-knockout mice. Surprisingly, expression of Se-dependent 5'-deiodinase type 1 was only slightly altered in liver, kidney, or thyroid at postnatal d 60, and 5'-deiodinase type 2 activity in brain was normal in SePP-knockout mice. Thyroid gland morphology, thyroid glutathione peroxidase activity, thyroid Se concentration, and serum levels of TSH, T4, or T3 were within normal range. Pituitary TSHbeta transcripts and hepatic 5'-deiodinase type 1 mRNA levels were unchanged, indicating regular T3 bioactivity in thyrotropes and hepatocytes. Cerebellar granule cell migration as a sensitive indicator of local T3 action during development was undisturbed. Collectively, these findings demonstrate that low levels of serum Se or SePP in the absence of other challenges do not necessarily interfere with regular functioning of the TH axis. 5'-deiodinase isozymes are preferentially supplied, and Se-dependent enzymes in the thyroid are even less-dependent on serum levels of Se or SePP than in brain. This indicates a top priority of the thyroid gland and its selenoenzymes with respect to the hierarchical Se supply within the organism. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/16322066/Synthesis_and_metabolism_of_thyroid_hormones_is_preferentially_maintained_in_selenium_deficient_transgenic_mice_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2005-1089 DB - PRIME DP - Unbound Medicine ER -