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Angiotensin-mediated increase in renal sympathetic nerve discharge within the PVN: role of nitric oxide.
Am J Physiol Regul Integr Comp Physiol. 2006 Apr; 290(4):R1035-43.AJ

Abstract

The paraventricular nucleus (PVN) of the hypothalamus is known to be an important site of integration in the central nervous system for sympathetic outflow. ANG II and nitric oxide (NO) play an important role in regulation of sympathetic nerve activity. The purpose of the present study was to examine how the interaction between NO and ANG II within the PVN affects sympathetic outflow in rats. Renal sympathetic nerve discharge (RSND), arterial blood pressure (AP), and heart rate (HR) were measured in response to administration of ANG II and N(G)-monomethyl-l-arginine (L-NMMA) into the PVN. Microinjection of ANG II (0.05, 0.5, and 1.0 nmol) into the PVN increased RSND, AP, and HR in a dose-dependent manner, resulting in increases of 53 +/- 9%, 19 +/- 3 mmHg, and 32 +/- 12 beats/min from baseline, respectively, at the highest dose. These responses were significantly enhanced by prior microinjection of L-NMMA and were blocked by losartan, an ANG II type 1 receptor antagonist. Similarly, administration of antisense to neuronal NO synthase within the PVN also potentiated the ANG II responses. Conversely, overexpression of neuronal NOS within the PVN with adenoviral gene transfer significantly attenuated ANG II responses. Push-pull administration of ANG II (1 nmol) into the PVN induced an increase in NO release. Our data indicate that ANG II type 1 receptors within the PVN mediate an excitatory effect on RSND, AP, and HR. NO in the PVN, which can be induced by ANG II stimulation, in turn inhibits the ANG II-mediated increase in sympathetic nerve activity. This negative-feedback mechanism within the PVN may play an important role in maintaining the overall balance and tone of sympathetic outflow.

Authors+Show Affiliations

Division of Basic Biomedical Science, College of Medicine, University of South Dakota, Vermillion, South Dakota, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16322353

Citation

Li, Yi-Fan, et al. "Angiotensin-mediated Increase in Renal Sympathetic Nerve Discharge Within the PVN: Role of Nitric Oxide." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 290, no. 4, 2006, pp. R1035-43.
Li YF, Wang W, Mayhan WG, et al. Angiotensin-mediated increase in renal sympathetic nerve discharge within the PVN: role of nitric oxide. Am J Physiol Regul Integr Comp Physiol. 2006;290(4):R1035-43.
Li, Y. F., Wang, W., Mayhan, W. G., & Patel, K. P. (2006). Angiotensin-mediated increase in renal sympathetic nerve discharge within the PVN: role of nitric oxide. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 290(4), R1035-43.
Li YF, et al. Angiotensin-mediated Increase in Renal Sympathetic Nerve Discharge Within the PVN: Role of Nitric Oxide. Am J Physiol Regul Integr Comp Physiol. 2006;290(4):R1035-43. PubMed PMID: 16322353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Angiotensin-mediated increase in renal sympathetic nerve discharge within the PVN: role of nitric oxide. AU - Li,Yi-Fan, AU - Wang,Wei, AU - Mayhan,William G, AU - Patel,Kaushik P, Y1 - 2005/12/01/ PY - 2005/12/3/pubmed PY - 2006/6/10/medline PY - 2005/12/3/entrez SP - R1035 EP - 43 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am J Physiol Regul Integr Comp Physiol VL - 290 IS - 4 N2 - The paraventricular nucleus (PVN) of the hypothalamus is known to be an important site of integration in the central nervous system for sympathetic outflow. ANG II and nitric oxide (NO) play an important role in regulation of sympathetic nerve activity. The purpose of the present study was to examine how the interaction between NO and ANG II within the PVN affects sympathetic outflow in rats. Renal sympathetic nerve discharge (RSND), arterial blood pressure (AP), and heart rate (HR) were measured in response to administration of ANG II and N(G)-monomethyl-l-arginine (L-NMMA) into the PVN. Microinjection of ANG II (0.05, 0.5, and 1.0 nmol) into the PVN increased RSND, AP, and HR in a dose-dependent manner, resulting in increases of 53 +/- 9%, 19 +/- 3 mmHg, and 32 +/- 12 beats/min from baseline, respectively, at the highest dose. These responses were significantly enhanced by prior microinjection of L-NMMA and were blocked by losartan, an ANG II type 1 receptor antagonist. Similarly, administration of antisense to neuronal NO synthase within the PVN also potentiated the ANG II responses. Conversely, overexpression of neuronal NOS within the PVN with adenoviral gene transfer significantly attenuated ANG II responses. Push-pull administration of ANG II (1 nmol) into the PVN induced an increase in NO release. Our data indicate that ANG II type 1 receptors within the PVN mediate an excitatory effect on RSND, AP, and HR. NO in the PVN, which can be induced by ANG II stimulation, in turn inhibits the ANG II-mediated increase in sympathetic nerve activity. This negative-feedback mechanism within the PVN may play an important role in maintaining the overall balance and tone of sympathetic outflow. SN - 0363-6119 UR - https://www.unboundmedicine.com/medline/citation/16322353/Angiotensin_mediated_increase_in_renal_sympathetic_nerve_discharge_within_the_PVN:_role_of_nitric_oxide_ L2 - https://journals.physiology.org/doi/10.1152/ajpregu.00338.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -