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Carnitine/organic cation transporter OCTN2-mediated transport of carnitine in primary-cultured epididymal epithelial cells.
Reproduction. 2005 Dec; 130(6):931-7.R

Abstract

Carnitine is essential for the acquisition of motility and maturation of spermatozoa in the epididymis, and is accumulated in epididymal fluid. In this study, carnitine transport into primary-cultured rat epididymal epithelial cells was characterized to clarify the nature of the transporter molecules involved. Uptake of carnitine by primary-cultured epididymal epithelial cells was time, Na(+) and concentration dependent. Kinetic analysis of carnitine uptake by the cells revealed the involvement of high- and low-affinity transport systems with Km values of 21 microM and 2.2 mM respectively. The uptake of carnitine by the cells was significantly reduced by inhibitors of carnitine/organic cation transporter (OCTN2), such as carnitine analogues and cationic compounds. In RT-PCR analysis, OCTN2 expression was detected. These results demonstrated that the high-affinity carnitine transporter OCTN2, which is localized at the basolateral membrane of epididymal epithelial cells, mediates carnitine supply into those cells from the systemic circulation as the first step of permeation from blood to spermatozoa.

Authors+Show Affiliations

Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16322553

Citation

Kobayashi, Daisuke, et al. "Carnitine/organic Cation Transporter OCTN2-mediated Transport of Carnitine in Primary-cultured Epididymal Epithelial Cells." Reproduction (Cambridge, England), vol. 130, no. 6, 2005, pp. 931-7.
Kobayashi D, Irokawa M, Maeda T, et al. Carnitine/organic cation transporter OCTN2-mediated transport of carnitine in primary-cultured epididymal epithelial cells. Reproduction. 2005;130(6):931-7.
Kobayashi, D., Irokawa, M., Maeda, T., Tsuji, A., & Tamai, I. (2005). Carnitine/organic cation transporter OCTN2-mediated transport of carnitine in primary-cultured epididymal epithelial cells. Reproduction (Cambridge, England), 130(6), 931-7.
Kobayashi D, et al. Carnitine/organic Cation Transporter OCTN2-mediated Transport of Carnitine in Primary-cultured Epididymal Epithelial Cells. Reproduction. 2005;130(6):931-7. PubMed PMID: 16322553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carnitine/organic cation transporter OCTN2-mediated transport of carnitine in primary-cultured epididymal epithelial cells. AU - Kobayashi,Daisuke, AU - Irokawa,Masanori, AU - Maeda,Tomoji, AU - Tsuji,Akira, AU - Tamai,Ikumi, PY - 2005/12/3/pubmed PY - 2006/3/10/medline PY - 2005/12/3/entrez SP - 931 EP - 7 JF - Reproduction (Cambridge, England) JO - Reproduction VL - 130 IS - 6 N2 - Carnitine is essential for the acquisition of motility and maturation of spermatozoa in the epididymis, and is accumulated in epididymal fluid. In this study, carnitine transport into primary-cultured rat epididymal epithelial cells was characterized to clarify the nature of the transporter molecules involved. Uptake of carnitine by primary-cultured epididymal epithelial cells was time, Na(+) and concentration dependent. Kinetic analysis of carnitine uptake by the cells revealed the involvement of high- and low-affinity transport systems with Km values of 21 microM and 2.2 mM respectively. The uptake of carnitine by the cells was significantly reduced by inhibitors of carnitine/organic cation transporter (OCTN2), such as carnitine analogues and cationic compounds. In RT-PCR analysis, OCTN2 expression was detected. These results demonstrated that the high-affinity carnitine transporter OCTN2, which is localized at the basolateral membrane of epididymal epithelial cells, mediates carnitine supply into those cells from the systemic circulation as the first step of permeation from blood to spermatozoa. SN - 1470-1626 UR - https://www.unboundmedicine.com/medline/citation/16322553/Carnitine/organic_cation_transporter_OCTN2_mediated_transport_of_carnitine_in_primary_cultured_epididymal_epithelial_cells_ L2 - https://rep.bioscientifica.com/doi/10.1530/rep.1.00737 DB - PRIME DP - Unbound Medicine ER -