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Gene expression of cytokines and growth factors in the lungs after paraquat administration in mice.
Leg Med (Tokyo). 2006 Mar; 8(2):102-9.LM

Abstract

It is well known that the intake of paraquat (PQ), an herbicide, causes severe lung injury at chronic phases. We examined the intrapulmonary gene expression of cytokines and growth factors after PQ administration. To induce lung injury, C57BL/6 mice were intraperitoneally injected twice a week with 20 mg/kg of PQ. Histopathologically, at the early phase, lots of alveolar spaces contained edematous fluid. At 3 weeks after PQ challenge, a marked thickening of the alveolar walls with the accumulation of macrophages and T cells was found. Azan staining revealed the patchy distribution of collagen accumulation, indicating pulmonary fibrosis. Consistently, intrapulmonary hydroxyproline contents were significantly elevated, compared with the controls. Semi-quantitative RT-PCR analysis demonstrated that the gene expression of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 were significantly increased at 3 weeks after PQ challenge compared with the controls. The mRNA expression of macrophage inflammatory protein (MIP)-1alpha and MIP-2 was significantly enhanced at 1 and 2 weeks after PQ treatment, respectively. Moreover, PQ-treated mice showed enhanced gene expression of fibrogenic growth factors such as transforming growth factor-beta, platelet-derived growth factor-A, acidic fibroblast growth factor, and hepatoctyte growth factor at 2 and/or 3 weeks after PQ challenge. The synergistic effects of these molecules are presumed to cause pulmonary fibrosis due to PQ challenge.

Authors+Show Affiliations

Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8509, Japan. iyuko@wakayama-med.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16324872

Citation

Ishida, Yuko, et al. "Gene Expression of Cytokines and Growth Factors in the Lungs After Paraquat Administration in Mice." Legal Medicine (Tokyo, Japan), vol. 8, no. 2, 2006, pp. 102-9.
Ishida Y, Takayasu T, Kimura A, et al. Gene expression of cytokines and growth factors in the lungs after paraquat administration in mice. Leg Med (Tokyo). 2006;8(2):102-9.
Ishida, Y., Takayasu, T., Kimura, A., Hayashi, T., Kakimoto, N., Miyashita, T., & Kondo, T. (2006). Gene expression of cytokines and growth factors in the lungs after paraquat administration in mice. Legal Medicine (Tokyo, Japan), 8(2), 102-9.
Ishida Y, et al. Gene Expression of Cytokines and Growth Factors in the Lungs After Paraquat Administration in Mice. Leg Med (Tokyo). 2006;8(2):102-9. PubMed PMID: 16324872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression of cytokines and growth factors in the lungs after paraquat administration in mice. AU - Ishida,Yuko, AU - Takayasu,Tatsunori, AU - Kimura,Akihiko, AU - Hayashi,Takahito, AU - Kakimoto,Nobuyuku, AU - Miyashita,Tomoko, AU - Kondo,Toshikazu, Y1 - 2005/12/01/ PY - 2005/07/11/received PY - 2005/08/17/revised PY - 2005/08/18/accepted PY - 2005/12/6/pubmed PY - 2006/8/12/medline PY - 2005/12/6/entrez SP - 102 EP - 9 JF - Legal medicine (Tokyo, Japan) JO - Leg Med (Tokyo) VL - 8 IS - 2 N2 - It is well known that the intake of paraquat (PQ), an herbicide, causes severe lung injury at chronic phases. We examined the intrapulmonary gene expression of cytokines and growth factors after PQ administration. To induce lung injury, C57BL/6 mice were intraperitoneally injected twice a week with 20 mg/kg of PQ. Histopathologically, at the early phase, lots of alveolar spaces contained edematous fluid. At 3 weeks after PQ challenge, a marked thickening of the alveolar walls with the accumulation of macrophages and T cells was found. Azan staining revealed the patchy distribution of collagen accumulation, indicating pulmonary fibrosis. Consistently, intrapulmonary hydroxyproline contents were significantly elevated, compared with the controls. Semi-quantitative RT-PCR analysis demonstrated that the gene expression of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 were significantly increased at 3 weeks after PQ challenge compared with the controls. The mRNA expression of macrophage inflammatory protein (MIP)-1alpha and MIP-2 was significantly enhanced at 1 and 2 weeks after PQ treatment, respectively. Moreover, PQ-treated mice showed enhanced gene expression of fibrogenic growth factors such as transforming growth factor-beta, platelet-derived growth factor-A, acidic fibroblast growth factor, and hepatoctyte growth factor at 2 and/or 3 weeks after PQ challenge. The synergistic effects of these molecules are presumed to cause pulmonary fibrosis due to PQ challenge. SN - 1344-6223 UR - https://www.unboundmedicine.com/medline/citation/16324872/Gene_expression_of_cytokines_and_growth_factors_in_the_lungs_after_paraquat_administration_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1344-6223(05)00117-3 DB - PRIME DP - Unbound Medicine ER -