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Impact of halides on the simultaneous separation of aromatic amines and their acidic metabolites by capillary electrophoresis with laser-induced native fluorescence detection under acidic conditions.
J Chromatogr A. 2006 Jan 13; 1102(1-2):302-8.JC

Abstract

This paper describes a simple, sensitive, efficient, and rapid method for simultaneous analysis of biologically active amines and acids by capillary electrophoresis in conjunction with laser-induced native fluorescence detection (CE-LINF) using a diode pumped solid state nanolaser at 266 nm. In order to optimize resolution of the amines that were prepared in 10.0 mM formate-Tris (FT) solutions, 10.0 mM FT solutions with and without containing halides were used to fill the capillary and reservoirs, respectively. The electrophoretic mobilities of tryptamine (TA) and serotonin (5-HT) at pH 4.0 decrease with the increase in halide concentration (0-10.0 mM). Taken together with a great effect of iodide than other halides, we suggest that the formation of ion pairs is a main contributor for altering the migration of the amines. In order to simultaneously analyze the amines and their metabolites (acids) at low pH, a high bulk EOF is required. The analysis of 10 anlytes including amines and acids was completed within 12 min by CE-LINF using a capillary treated with 0.5M NaOH and then filled with 10.0 mM FT solutions (pH 4.0) containing 10.0 mM KCl prior to analysis. The limits of detection for TA and 5-hydroxyindole-3-acetic acid (5-HIAA) are 0.12 and 6.0 nM, respectively. The present method has been further validated by analyzing urine samples, with an RSD less than 3.1% (migration times) and 3.9% (concentration).

Authors+Show Affiliations

Department of Applied Chemistry, Fooyin University, 151 Chin-Hsueh Road, Ta-Liao Hsiang, 831 Kaohsiung Hsien, Taiwan. sc126@mail.fy.edu.twNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16325830

Citation

Hsieh, Ming-Mu, and Huan-Tsung Chang. "Impact of Halides On the Simultaneous Separation of Aromatic Amines and Their Acidic Metabolites By Capillary Electrophoresis With Laser-induced Native Fluorescence Detection Under Acidic Conditions." Journal of Chromatography. A, vol. 1102, no. 1-2, 2006, pp. 302-8.
Hsieh MM, Chang HT. Impact of halides on the simultaneous separation of aromatic amines and their acidic metabolites by capillary electrophoresis with laser-induced native fluorescence detection under acidic conditions. J Chromatogr A. 2006;1102(1-2):302-8.
Hsieh, M. M., & Chang, H. T. (2006). Impact of halides on the simultaneous separation of aromatic amines and their acidic metabolites by capillary electrophoresis with laser-induced native fluorescence detection under acidic conditions. Journal of Chromatography. A, 1102(1-2), 302-8.
Hsieh MM, Chang HT. Impact of Halides On the Simultaneous Separation of Aromatic Amines and Their Acidic Metabolites By Capillary Electrophoresis With Laser-induced Native Fluorescence Detection Under Acidic Conditions. J Chromatogr A. 2006 Jan 13;1102(1-2):302-8. PubMed PMID: 16325830.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of halides on the simultaneous separation of aromatic amines and their acidic metabolites by capillary electrophoresis with laser-induced native fluorescence detection under acidic conditions. AU - Hsieh,Ming-Mu, AU - Chang,Huan-Tsung, Y1 - 2005/12/01/ PY - 2005/06/27/received PY - 2005/10/28/revised PY - 2005/11/01/accepted PY - 2005/12/6/pubmed PY - 2006/3/10/medline PY - 2005/12/6/entrez SP - 302 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1102 IS - 1-2 N2 - This paper describes a simple, sensitive, efficient, and rapid method for simultaneous analysis of biologically active amines and acids by capillary electrophoresis in conjunction with laser-induced native fluorescence detection (CE-LINF) using a diode pumped solid state nanolaser at 266 nm. In order to optimize resolution of the amines that were prepared in 10.0 mM formate-Tris (FT) solutions, 10.0 mM FT solutions with and without containing halides were used to fill the capillary and reservoirs, respectively. The electrophoretic mobilities of tryptamine (TA) and serotonin (5-HT) at pH 4.0 decrease with the increase in halide concentration (0-10.0 mM). Taken together with a great effect of iodide than other halides, we suggest that the formation of ion pairs is a main contributor for altering the migration of the amines. In order to simultaneously analyze the amines and their metabolites (acids) at low pH, a high bulk EOF is required. The analysis of 10 anlytes including amines and acids was completed within 12 min by CE-LINF using a capillary treated with 0.5M NaOH and then filled with 10.0 mM FT solutions (pH 4.0) containing 10.0 mM KCl prior to analysis. The limits of detection for TA and 5-hydroxyindole-3-acetic acid (5-HIAA) are 0.12 and 6.0 nM, respectively. The present method has been further validated by analyzing urine samples, with an RSD less than 3.1% (migration times) and 3.9% (concentration). SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16325830/Impact_of_halides_on_the_simultaneous_separation_of_aromatic_amines_and_their_acidic_metabolites_by_capillary_electrophoresis_with_laser_induced_native_fluorescence_detection_under_acidic_conditions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(05)02073-X DB - PRIME DP - Unbound Medicine ER -