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Immunosensor for the detection of Vibrio cholerae O1 using surface plasmon resonance.
Biosens Bioelectron. 2006 Jun 15; 21(12):2315-9.BB

Abstract

An immunosensor for the detection of Vibrio cholerae O1 was developed on the basis of surface plasmon resonance (SPR). A protein G layer was fabricated by means of the chemical coupling between the free amine (-NH2) groups of protein G and the activated carboxyl groups present on a self-assembled monolayer (SAM) consisting of a mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol (molar ratio of 1:2). A monoclonal antibody, which was confirmed to be specific to V. cholera O1 by the Western blotting technique, was immobilized on the protein G layer. The formation of the SAM, the protein G layer and the sequential binding of the antibody against V. cholera O1 were investigated with SPR spectroscopy. As the number of fabricated layers increased, the minimum angle of plasmon resonance was increased accordingly. The target bacteria, V. cholera O1, was measured with the fabricated immunosensor, whose detection range was between 10(5) and 10(9) cells/mL.

Authors+Show Affiliations

Jyoung JY
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-Dong, Mapo-Gu, Seoul 121-742, Republic of Korea. jyoung91@hanmir.com
Hong S
No affiliation info available
Lee W
No affiliation info available
Choi JW
No affiliation info available

MeSH

Biosensing TechniquesColony Count, MicrobialImmunoassayNerve Tissue ProteinsSurface Plasmon ResonanceVibrio cholerae O1

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16326089

Citation

Jyoung, Jy-Young, et al. "Immunosensor for the Detection of Vibrio Cholerae O1 Using Surface Plasmon Resonance." Biosensors & Bioelectronics, vol. 21, no. 12, 2006, pp. 2315-9.
Jyoung JY, Hong S, Lee W, et al. Immunosensor for the detection of Vibrio cholerae O1 using surface plasmon resonance. Biosens Bioelectron. 2006;21(12):2315-9.
Jyoung, J. Y., Hong, S., Lee, W., & Choi, J. W. (2006). Immunosensor for the detection of Vibrio cholerae O1 using surface plasmon resonance. Biosensors & Bioelectronics, 21(12), 2315-9.
Jyoung JY, et al. Immunosensor for the Detection of Vibrio Cholerae O1 Using Surface Plasmon Resonance. Biosens Bioelectron. 2006 Jun 15;21(12):2315-9. PubMed PMID: 16326089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosensor for the detection of Vibrio cholerae O1 using surface plasmon resonance. AU - Jyoung,Jy-Young, AU - Hong,SaHyun, AU - Lee,Woochang, AU - Choi,Jeong-Woo, Y1 - 2005/12/01/ PY - 2005/06/16/received PY - 2005/10/12/revised PY - 2005/10/25/accepted PY - 2005/12/6/pubmed PY - 2006/7/18/medline PY - 2005/12/6/entrez SP - 2315 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 12 N2 - An immunosensor for the detection of Vibrio cholerae O1 was developed on the basis of surface plasmon resonance (SPR). A protein G layer was fabricated by means of the chemical coupling between the free amine (-NH2) groups of protein G and the activated carboxyl groups present on a self-assembled monolayer (SAM) consisting of a mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol (molar ratio of 1:2). A monoclonal antibody, which was confirmed to be specific to V. cholera O1 by the Western blotting technique, was immobilized on the protein G layer. The formation of the SAM, the protein G layer and the sequential binding of the antibody against V. cholera O1 were investigated with SPR spectroscopy. As the number of fabricated layers increased, the minimum angle of plasmon resonance was increased accordingly. The target bacteria, V. cholera O1, was measured with the fabricated immunosensor, whose detection range was between 10(5) and 10(9) cells/mL. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16326089/Immunosensor_for_the_detection_of_Vibrio_cholerae_O1_using_surface_plasmon_resonance_ DB - PRIME DP - Unbound Medicine ER -