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In vivo and in vitro immunosuppressive effects of benzo[k]fluoranthene in female Balb/c mice.
J Toxicol Environ Health A. 2005 Dec 10; 68(23-24):2033-50.JT

Abstract

Although polycyclic aromatic hydrocarbons (PAHs) have been known to suppress immune responses, few studies have addressed the immunotoxicity of benzo[k]fluoranthene (B[k]F). In this study, we investigated the immunosuppression by B[k]F, both in vivo and in vitro, in female BALB/c mice. To assess the effects of B[k]F on humoral immunity as splenic antibody response to sheep red blood cells (SRBCs), B[k]F was given a single dose or once daily for 7 consecutive days po with 30, 60, and 120 micromol/kg. B[k]F reduced the number of antibody-forming cells (AFCs) in a dose-dependent manner. Subacute treatment with B[k]F caused weight increases in liver and decreases in spleen and thymus. The number of AFCs was dramatically decreased by B[k]F in a dose-dependent manner. In a subsequent study, mice were subacutely exposed to the same doses of B[k]F without an immunization with SRBCs, followed by splenic and thymic lymphocyte phenotypings using a flow cytometry and ex vivo mitogen-stimulated proliferation. B[k]F-exposed mice exhibited reduced splenic and thymic cellularity, decreased numbers of total T cells, CD4(+) cells, and CD8(+) cells in spleen, and immature CD4(+)CD8(+) cells, CD4(+)CD8(-) cells, and CD8(+)CD4(-) cells in thymus. The number of CD4(+) IL-2(+) cells was reduced by about 11%, 31%, and 53% following exposure of mice to 30, 60, and 120 micromol/kg of B[k]F, respectively. In the ex vivo lymphocyte proliferation assay, B[k]F inhibited splenocyte proliferation by LPS and Con A. In the in vitro mitogen-stimulated proliferation by untreated splenic suspensions, B[k]F only suppressed splenocyte proliferation to LPS. These results suggested that B[k]F-induced immunosuppression might be mediated, at least in part, through the IL-2 production, and caused by mechanisms associated with metabolic processes.

Authors+Show Affiliations

College of Pharmacy, Yeungnam University, Kyungsan, South Korea. taecheon@yumail.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16326422

Citation

Jeon, Tae Won, et al. "In Vivo and in Vitro Immunosuppressive Effects of Benzo[k]fluoranthene in Female Balb/c Mice." Journal of Toxicology and Environmental Health. Part A, vol. 68, no. 23-24, 2005, pp. 2033-50.
Jeon TW, Jin CH, Lee SK, et al. In vivo and in vitro immunosuppressive effects of benzo[k]fluoranthene in female Balb/c mice. J Toxicol Environ Health A. 2005;68(23-24):2033-50.
Jeon, T. W., Jin, C. H., Lee, S. K., Lee, D. W., Hyun, S. H., Kim, G. H., Jun, I. H., Lee, B. M., Yum, Y. N., Kim, J. K., Kim, O. H., & Jeong, T. C. (2005). In vivo and in vitro immunosuppressive effects of benzo[k]fluoranthene in female Balb/c mice. Journal of Toxicology and Environmental Health. Part A, 68(23-24), 2033-50.
Jeon TW, et al. In Vivo and in Vitro Immunosuppressive Effects of Benzo[k]fluoranthene in Female Balb/c Mice. J Toxicol Environ Health A. 2005 Dec 10;68(23-24):2033-50. PubMed PMID: 16326422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo and in vitro immunosuppressive effects of benzo[k]fluoranthene in female Balb/c mice. AU - Jeon,Tae Won, AU - Jin,Chun Hua, AU - Lee,Sang Kyu, AU - Lee,Dong Wook, AU - Hyun,Sun Hee, AU - Kim,Ghee Hwan, AU - Jun,In Hye, AU - Lee,Byung Mu, AU - Yum,Young Na, AU - Kim,Jun Kyou, AU - Kim,Ok Hee, AU - Jeong,Tae Cheon, PY - 2005/12/6/pubmed PY - 2006/1/7/medline PY - 2005/12/6/entrez SP - 2033 EP - 50 JF - Journal of toxicology and environmental health. Part A JO - J Toxicol Environ Health A VL - 68 IS - 23-24 N2 - Although polycyclic aromatic hydrocarbons (PAHs) have been known to suppress immune responses, few studies have addressed the immunotoxicity of benzo[k]fluoranthene (B[k]F). In this study, we investigated the immunosuppression by B[k]F, both in vivo and in vitro, in female BALB/c mice. To assess the effects of B[k]F on humoral immunity as splenic antibody response to sheep red blood cells (SRBCs), B[k]F was given a single dose or once daily for 7 consecutive days po with 30, 60, and 120 micromol/kg. B[k]F reduced the number of antibody-forming cells (AFCs) in a dose-dependent manner. Subacute treatment with B[k]F caused weight increases in liver and decreases in spleen and thymus. The number of AFCs was dramatically decreased by B[k]F in a dose-dependent manner. In a subsequent study, mice were subacutely exposed to the same doses of B[k]F without an immunization with SRBCs, followed by splenic and thymic lymphocyte phenotypings using a flow cytometry and ex vivo mitogen-stimulated proliferation. B[k]F-exposed mice exhibited reduced splenic and thymic cellularity, decreased numbers of total T cells, CD4(+) cells, and CD8(+) cells in spleen, and immature CD4(+)CD8(+) cells, CD4(+)CD8(-) cells, and CD8(+)CD4(-) cells in thymus. The number of CD4(+) IL-2(+) cells was reduced by about 11%, 31%, and 53% following exposure of mice to 30, 60, and 120 micromol/kg of B[k]F, respectively. In the ex vivo lymphocyte proliferation assay, B[k]F inhibited splenocyte proliferation by LPS and Con A. In the in vitro mitogen-stimulated proliferation by untreated splenic suspensions, B[k]F only suppressed splenocyte proliferation to LPS. These results suggested that B[k]F-induced immunosuppression might be mediated, at least in part, through the IL-2 production, and caused by mechanisms associated with metabolic processes. SN - 1528-7394 UR - https://www.unboundmedicine.com/medline/citation/16326422/In_vivo_and_in_vitro_immunosuppressive_effects_of_benzo[k]fluoranthene_in_female_Balb/c_mice_ L2 - https://www.tandfonline.com/doi/full/10.1080/15287390491009147 DB - PRIME DP - Unbound Medicine ER -